Method for detecting belladonna alkaloids in animal tissues
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A technology of animal tissue and detection method is applied in the detection field of belladonna alkaloids, and achieves the effects of good reference value, broad market prospects and significant economic benefits
Pending Publication Date: 2022-04-29
JILIN ACAD OF AGRI SCI
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[0004] The purpose of the present invention is to overcome the defects of the prior art, provide a kind
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[0035] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples. For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions.
[0036] Instrument and equipment of the present invention:
[0037] 6410B liquid chromatography-tandem quadrupole mass spectrometer, Agilent, USA;
[0044] Standard substance name CAS number standard value Manufacturer Anisodamine 55869-99-3 10mg, ≥99.5...
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Abstract
The invention provides a method for detecting belladonna alkaloids in animal tissues, which adopts dispersive solid-phase extraction-high performance liquid chromatography triple quadrupole mass spectrometry to simultaneously determine anisodamine, racanisodamine, anisodine, atropine, scopolamine, tropine acid, noratropine and the like in the animal tissues by taking tropine as an internal standard substance. The pretreatment and separation detection conditions are optimized. A target compound is good in linearity within the range of 0.5 ng/L-200ng/L, the detection limit of the method is 0.05-0.1 mu g/kg, the quantification limit of the method is 0.2-0.5 mu g/kg, the matrix blank adding standard recovery rate is 80.2-119%, and the relative standard deviation of parallel determination is 1.13-14.5%. According to the method, the belladonna alkaloids in the animal tissues can be accurately measured, the measurement result is accurate and reliable, reliable data is provided for measurement of the belladonna alkaloids, and the method has remarkable economic benefits and wide market prospects. The method has a good reference value for guiding the measurement of drug residues.
Description
technical field [0001] The invention relates to the technical field of detection, in particular to a detection method of belladonna alkaloids in animal tissues. Background technique [0002] Toxic Solanaceae plants such as Datura, Hyacinth, and Belladonna contain a variety of toxic alkaloids, such as hyoscyamine, atropine, scopolamine, anisodamine, and anisodine. These alkaloids are collectively referred to as belladonna alkaloids, also known as tropane alkaloids. Belladonna alkaloids can interfere with the cerebral cortex, produce central inhibition, and competitively block the excitatory effect of M choline receptors on the respiratory center, causing dry mouth, dilated pupils, hoarseness, rapid heartbeat, vasodilation, and intermittent convulsions or convulsions and other phenomena, leading to central system disorder, mania, dizziness, hallucinations, general dosage can make people feel tired, and enter dreamless sleep. Symptoms such as coma, and eventually respiratory ...
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