41 results about "Tropane alkaloid" patented technology

The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

The present invention provides a method for the N-demethylation and / or N-acylation of an N-methylated heterocycle such as morphine alkaloids or tropane alkaloids. The method comprises reacting the heterocycle with an acylating agent in the presence of a metal catalyst.

The invention discloses a tropane alkaloid extraction technology, which comprises the following steps: crushing roots of the Tibetan medicine Anisodus tanguticus (Maxim.) Pascher and then extracting to obtain an extract, filtering the extract through a ceramic membrane to remove impurities such as vegetable protein, colloid, suspensions and the like to obtain a ceramic membrane clear liquid, removing impurities such as small molecular protein, pigments and the like through a ultrafiltration membrane so as to obtain a ultrafiltration membrane clear liquid, adsorbing Anisodus tanguticus (Maxim.)Pascher through macroporous adsorption resin, parsing through ethanol to obtain a macroporous adsorption resin parsing solution, concentrating through a nanofiltration membrane to obtain a nanofiltration membrane concentrated solution containing tropane alkaloid, and evaporating and crystallizing. The technical problem that impurities such as suspensions, colloid, protein, etc. in the extractionprocess of tropane alkaloid are easy to pollute macroporous adsorption resin in the subsequent process is solved. In comparison with the prior art, the equipment process is simple, degree of automation is high, the control process is simple, and production efficiency and product recovery rate are improved.

The present invention provides a method for the N-demethylation and N-functionalization of an N-methylated heterocycle such as a morphine alkaloid or tropane alkaloid. The method comprises reacting the heterocycle with an functionalization agent in the presence of a transition metal catalyst in air or in the presence of an oxidant.

The present invention provides a method for the N-demethylation of an N-methylated heterocycle, particularly a morphine or tropane alkaloid or derivative thereof. The method comprises reacting the heterocycle with a metal catalyst and a solvent in the presence of an oxidizing agent.

Provided herein, among other things, is an engineered non-plant cell that produces a tropane alkaloid product, a precursor of a tropane alkaloid product, or a derivative of a tropane alkaloid product. A method for producing a tropane alkaloid, a precursor of a tropane alkaloid product, or a derivative of a tropane alkaloid product that makes use of the cell is also described.

The invention discloses a radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, protein which is encoded by the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, and the use thereof, which fills a gap that the 1,4-putrescine-nitrogen-methyltransferase gene is separated and cloned from radix anisodii acutanguli which is a specific medicinal plant in Yunnan, China. The radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1 which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The 1,4-putrescine-nitrogen-methyltransferase gene which is provided by the invention has prominent effect of increasing the content of tropane alkaloid in plants such as the radix anisodii acutanguli and the like through the genetic engineering technology and can be widely applied in improving the quality of resource plants which produce tropane alkaloid.

The invention discloses anisodus tropinone reductase II gene and the protein and the use of the code thereof, and fills the blank of separating and cloning the tropinone reductase II gene from unique medicinal plants in Yunnan, China. The anisodus tropinone reductase II gene provided by the invention has nucleotide sequence shown in SEQ ID No.1, and the protein of the gene code has amino acid sequence shown in SEQ ID No.2. The anisodus tropinone reductase II gene provided by the invention has prominent effect for improving the content of tropane alkaloid in anisodus plants, etc. through an antisense gene technology, thereby being widely applied to the qauality improvement of resource plants producing tropane alkaloid.

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

The invention discloses a process for extracting scopolane alkaloids, which comprises crushing and leaching the roots of scopolamine from Tibetan medicine scopolamine, and filtering the obtained extract through a ceramic membrane to remove vegetable protein. , colloid, suspended solids and other impurities to obtain ceramic membrane clear liquid, and then pass through ultrafiltration membrane to remove impurities such as small molecular proteins and pigments to obtain ultrafiltration membrane clear liquid, then pass through macroporous adsorption resin to absorb anisodamine , analyzed with ethanol to obtain the desorption solution of the macroporous adsorption resin, and then concentrated through the nanofiltration membrane to obtain the concentrated solution of the nanofiltration membrane containing tropane alkaloids, which is used for evaporation and crystallization in the next step. The invention solves the technical problem that impurities such as suspended solids, colloids and proteins in the extraction process of tropane alkaloids are easily polluted in subsequent macroporous adsorption resins. Compared with the prior art, the present invention has simple equipment process, high degree of automation, simple control process and improved production efficiency and product recovery rate.

The invention discloses an application of arabidopsis thaliana UGT74F2 in catalyzing phenyllactic acid to be subjected to glycosyl transfer reaction to synthesize phenyllactoyl glucose. Research finds that UGT74F2 from arabidopsis thaliana can catalyze phenyllactic acid to synthesize phenyllactoyl glucose, and the catalytic activity of the UGT74F2 is higher than that of AbUGT1 reported at present and used for tropine alkaloid synthesis. The UGT74F2 can be used for tropane alkaloid metabolic engineering to improve the alkaloid content, the application value of UGT74F2 is widened, and the UGT74F2 has very important significance on medicinal TA metabolic engineering and synthetic biology.

The invention relates to the technical field of chemical synthesis, and a tropane alkaloid and a synthesis method thereof are particularly disclosed. The structure formula (I) of the target product scopolamine alkaloid is described in the description, the compound of formula (I) is a compound containing a five-membered aza-bridged ring skeleton in which that nitrogen atom is protected by 2-pyridinecarboxylic acid; R1 is hydrogen or methoxycarbonyl; R2 is hydrogen or substituted phenyl, and the tropane alkaloid is racemic. Compounds of formula (I) have a significant relaxing effect on KCl-precontracted rat thoracic aorta, suggesting that compounds of formula (I) have a modulatory effect on vascular endothelial cells or smooth muscle cells, and may inhibit the occurrence and development of hypertension.

The invention discloses a radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, protein which is encoded by the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferasegene1, and the use thereof, which fills a gap that the 1,4-putrescine-nitrogen-methyltransferase gene is separated and cloned from radix anisodii acutanguli which is a specific medicinal plant in Yunnan, China. The radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1 which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of aminoacids, which is displayed in the SEQ ID No.2. The 1,4-putrescine-nitrogen-methyltransferase gene which is provided by the invention has prominent effect of increasing the content of tropane alkaloid in plants such as the radix anisodii acutanguli and the like through the genetic engineering technology and can be widely applied in improving the quality of resource plants which produce tropane alkaloid.