Method for inducing hairy roots of anisodus acutangulus C.Y.Wu et C.Chen

A hairy root and seed technology, applied in the field of plant biology, can solve problems such as the threat of wild and valuable Chinese medicinal materials resources, and achieve the effects of ensuring sustainable utilization, reducing production costs and having broad market prospects.

Inactive Publication Date: 2015-11-25
GUIZHOU UNIV
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Problems solved by technology

[0003] The development and utilization of natural medicines has become an important field of mode

Method used

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  • Method for inducing hairy roots of anisodus acutangulus C.Y.Wu et C.Chen
  • Method for inducing hairy roots of anisodus acutangulus C.Y.Wu et C.Chen
  • Method for inducing hairy roots of anisodus acutangulus C.Y.Wu et C.Chen

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Embodiment Construction

[0024] Embodiments of the invention: method for inducing three-point three-hair root

[0025] Material among the present invention is three-thirds ( Anisodus acutangulus C.Y.WuetC.Chen) seeds were provided by the Kunming Institute of Botany, Chinese Academy of Sciences, and the three-point three-point sterile tissue culture system was established by the Plant Physiology Laboratory of the School of Life Sciences, Guizhou University; Agrobacterium rhizogenes A4 was preserved by the Plant Physiology Laboratory of the School of Life Sciences, Guizhou University ( This strain is a well-known strain and can be obtained by most laboratories).

[0026] (1) Activate Agrobacterium rhizogenes and prepare transformed bacterial solution: under sterile conditions, use an inoculation loop to pick out the A4 strain of Agrobacterium rhizogenes frozen in a low-temperature refrigerator at -20°C, and use LB+Kan50mg / L solid After streaking on the plate, place it in a 28°C constant temperature inc...

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Abstract

The invention discloses a method for inducing hairy roots of anisodus acutangulus C.Y.Wu et C.Chen and mainly provides a technology for inducing the hairy roots of the anisodus acutangulus C.Y.Wu et C.Chen by agrobacterium rhizogene mediated transformation. The method particularly involves agrobacterium rhizogene activation, transformed bacterium liquid preparation, explant transformation, hairy root subculturing, PCR (polymerase chain reaction) detection of the hairy roots and the like. The method is capable of achieving industrialized production of tropane alkaloids by means of high-efficiency induction and large-scale culture of the hairy roots of the anisodus acutangulus C.Y.Wu et C.Chen, and is of great significance to sustainable use of the anisodus acutangulus C.Y.Wu et C.Chen as a rare medicinal plant source.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to the generation of three-part three-leaf callus and the induced formation of hairy roots. Background technique [0002] three-thirds ( Anisodus acutangulus C.Y.WuetC.Chen) is a perennial rare herbal medicinal plant of Solanaceae unique to my country. It is used as medicine with roots, stems, leaves and seeds. Poisoning, hence the name Sanfensan. Its roots, stems, leaves and seeds are rich in tropane alkaloids, mainly including hyoscyamine, anisodamine, scopolamine and anisodine, which have strong anticholinergic physiological activity and are used for various causes. Shock, adult respiratory distress syndrome, severe hepatitis and other diseases, among them, scopolamine and scopolamine are important anti-parasympathetic drugs, which are mainly used as sedatives in clinical practice, and the demand continues to increase. [0003] The development and utilization of natural medic...

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Application Information

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IPC IPC(8): C12N15/82A01H4/00A01H5/00
Inventor 张明生吕享杨小蕊曹然吴彦秋
Owner GUIZHOU UNIV
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