Scopoloa acutangula 1,4-tetramethylenediamine-nitrogen-methyltransferase 1 and its coding protein and application

Abstract

The invention discloses a radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, protein which is encoded by the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, and the use thereof, which fills a gap that the 1,4-putrescine-nitrogen-methyltransferase gene is separated and cloned from radix anisodii acutanguli which is a specific medicinal plant in Yunnan, China. The radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1 which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the radix anisodii acutanguli 1,4-putrescine-nitrogen-methyltransferase gene1, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The 1,4-putrescine-nitrogen-methyltransferase gene which is provided by the invention has prominent effect of increasing the content of tropane alkaloid in plants such as the radix anisodii acutanguli and the like through the genetic engineering technology and can be widely applied in improving the quality of resource plants which produce tropane alkaloid.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to 1,4-butanediamine-nitrogen-methyltransferase gene 1 expressed in Sanfensan and its encoded protein and application. Background technique

[0002] Hyoscyamine alkaloids, such as hyoscyamine and scopolamine, are mainly extracted from plants of the family Solanaceae such as belladonna, datura, hyoscyamine and three-point three, etc., and act on parasympathetic nerves in medical aspects A systemic anticholinergic drug with anesthesia, antispasmodic and analgesic functions. In addition, it also has the effect of improving microcirculation, and can be used clinically to treat diseases of microcirculation disorders. Thanks to the efforts of Chinese scholars, the clinical application of tropane alkaloids has been widely used in internal medicine, surgery, obstetrics and gynecology, neurology, dermatology, otolaryngology, etc., and can treat more than 100 diseases, and the market ...

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