Hyoscyamine aldehyde reductase and its application

A technology of reductase and hyospolamine, applied in the direction of oxidoreductase, application, enzyme, etc., can solve the problems of unclear biosynthesis pathway of secondary metabolites, complex process flow, and inability to realize chemical total synthesis, etc.
CN110452916AActive Publication Date: 2019-11-15SOUTHWEST UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2019-11-15

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Abstract

The present invention discloses a hyoscyamine aldehyde reductase synthase(HAR) and its application, the hyoscyamine aldehyde reductase synthase has an amino acid residue sequence shown as SEQ ID NO. 4, and a nucleotide sequence shown as SEQ ID NO. 3; the catalyzed reaction product hyoscyamine aldehyde after the prokaryotic expression is hyoscyamine, the hyoscyamine aldehyde is used to transform atropa belladonna, which can increase the content of hyoscyamine in atropa belladonna cell line, and has important meaning to increase the content of tropane alkaloids in atropa belladonna.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to scopolamine reductase, and also to the application of scopolamine reductase. Background technique

[0002] Tropane alkaloids (Tas) are a class of anticholinergic drugs with great medical value, widely used in anesthesia, analgesia, cough, asthma and anti-motion sickness, and also used to control stiffness in Parkinson's disease and tremors. Hyoscyamine and scopolamine are commonly used clinically, and the market demand for both is huge. Among them, scopolamine has weaker side effects, stronger drug effect, and more expensive price. At present, TAs are extracted from a few TAs resource plants of Solanaceae, including Atropa belladonna, Datura stramonium and Hyoscyamus niger. , is also a TAs drug source plant included in the Pharmacopoeia. The mass fraction of scopolamine in wild belladonna plants is 0.02%-0.17% (dry weight), and the content of scopolamine is extremely low, only 0.0...

Claims

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