6 results about "Aldehyde Reductase" patented technology

The invention discloses an aldehyde reductase mutant and application thereof in synthesis of a dexmethylphenidate hydrochloride intermediate, and belongs to the field of molecular biology and enzyme engineering. The aldehyde reductase mutant, polynucleotide for coding the mutant, and the recombinant expression vector can express the aldehyde reductase mutant and are used for constructing a recombinant cell or a recombinant strain for expressing the aldehyde reductase mutant. The provided aldehyde reductase mutant can catalyze 2-phenyl-2-((R)-piperidine-2)-acetaldehyde into (R)-2-phenyl-2-((R)-piperidine-2)-1-ethanol, especially improves the stereoselectivity of (R)-2-phenyl-2-((R)-piperidine-2)-1-ethanol, solves the problems of strict conditions, complex reaction and high cost in the existing synthesis method, and has a wide application prospect in the field of synthesis of (R)-2-phenyl-2-((R)-piperidine-2)-1-ethanol. Wide application prospects are realized.

The present application relates to the technical field of genetic engineering, and discloses a recombinant Escherichia coli for high-yield O-acetyl-L-homoserine and application thereof. The recombinant Escherichia coli for high-yield O-acetyl-L-homoserine is obtained by the following combination of modification strategies: in Escherichia coli W3110, a gene encoding homoserine O-acetyltransferase is integrated into a gene site encoding L-arginine ABC transporter ATP binding subunit, then a gene encoding phosphoacetyltransferase is knocked out, then a gene encoding transcriptional anti-terminator and mRNA stability regulator is knocked out, then a gene encoding ATP-NAD kinase is integrated into a gene site encoding NADPH-dependent aldehyde reductase, and finally a gene promoter of acetyl-CoA synthetase is replaced by a Ptrc promoter to obtain the recombinant Escherichia coli. The recombinant Escherichia coli is used for fermentation to produce O-acetyl-L-homoserine, and has the advantage of high yield. metx artp patz cspc ppnk yahk acs ​​​​​​​

The invention provides a novel reverse aldolase and reductase cascaded Bi-BDO independent synthesis pathway, and relates to the technical field of bioengineering, the pathway constructs a non-natural metabolism module composed of a modified reverse aldolase RA variant, 4-hydroxybutyraldehyde reductase AHR and a coenzyme cyclic regeneration enzyme, the RA variant performs hydrophobic modification on a Lys146 site, and the reverse aldolase and reductase cascaded Bi-BDO is obtained. Aldol condensation of a non-phosphorylated substrate hydroxyacetaldehyde is realized to generate a C4 intermediate, RA and AHR are physically anchored by utilizing an artificially synthesized scaffold protein, toxicity of the intermediate is avoided through a substrate channel effect, the intermediate is instantly reduced into 1, 4-butanediol, engineering bacteria of aldA and gapA genes are knocked out, a precursor is generated by utilizing a xylose way, and the 1, 4-butanediol is obtained. The method has the advantages that the method is simple in operation, the reduction reaction is driven by in-situ regeneration of NADPH by adding formate, finally, the high-purity product is obtained through macroporous adsorption resin enrichment and vacuum rectification, the TCA circulation decarboxylation step is omitted, the carbon conversion rate is close to the theoretical limit, effective decoupling of production and growth is achieved, and the yield and purity of Bi-BDO are remarkably improved.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty alcohol or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE), at least one gene enabling the expression of at least one carboxylic acid reductase (CAR) and at least one gene enabling the expression of an aldehyde reductase (ALDR). The present invention further provides methods for the manufacture of C6-C26 free fatty alcohols or mixtures thereof, as well as a free fatty alcohol composition obtained from these methods.

The application provides an engineering bacterium and a cell lysate thereof, and an application of the engineering bacterium in full-cell photocatalytic preparation of 1,3-propanediol, and belongs to the technical field of biotechnology. 12 The engineering bacterium comprises: a non-B 12 dependent glycerol dehydratase, a light-sensitive protein PSP2, a glycerol dehydratase activating enzyme, and an NADPH-dependent aldehyde reductase. The engineering bacterium provided in the application realizes catalysis of glycerol conversion under the condition of not depending on B 12 12, and drives the reductive activation of the glycerol dehydratase activating enzyme by using a light enzyme, so that the synthesis efficiency of 1,3-propanediol is finally improved, and the effect of coenzyme dependence and cost limitation of a traditional process is reduced.

The invention discloses a method for synthesizing raspberry ketone through chemical enzyme stage combination and application, and belongs to the technical field of organic synthesis and biological engineering. The raspberry ketone is obtained through whole-cell catalysis by taking recombinant engineering bacteria of co-expressed olefine aldehyde reductase and formate dehydrogenase genes as a biocatalyst. When the adding amount of a genetic engineering bacterium E.coli BL21 (DE3) pRSF-AtQOR (I56S / A69H / A118N)-LbFDH is 20 g / L, the substrate HBA is 100 g / L, the sodium formate is 125 g / L, the NADP < + > is 0.5 mM, the reaction temperature is 37 DEG C, the pH is 7.5, the reaction time is 12 h, and the rotating speed is 220 rpm, the raspberry ketone concentration can reach 82.23 g / L. The problems of serious environmental pollution of a chemical method and low yield of a biological method are solved.