Recombinant genetically engineered bacterium of co-expressing olefine aldehyde reductase and glucose dehydrogenase and application of recombinant genetically engineered bacterium

A technology of glucose dehydrogenase and genetically engineered bacteria, applied in genetic engineering, oxidoreductase, applications, etc., can solve the problems that have not yet been seen in co-expressing enaldehyde reductase and glucose dehydrogenase recombinant E. Large-scale industrial production, lower production cost, high vitality effect
CN110643556AInactive Publication Date: 2020-01-03ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2020-01-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a recombinant genetically engineered bacterium of co-expressing olefine aldehyde reductase and glucose dehydrogenase and application of the recombinant genetically engineered bacterium to catalyzes the synthesis of allylic alcohols with methylcrotonaldehyde. The genetically engineered bacterium is obtained by co-introducing olefine aldehyde reductase gene and D- glucose dehydrogenase into a host bacteria. A method has the advantages of being high in regioselectivity and high in activity. The 500mM substrate methylcrotonaldehyde is completely converted into the product of the allylic alcohols in 3.5h, no by-product saturated alcohol is detected during the reaction, and it is indicated that the method is efficient and specific in catalyzing the C=O hydrogenation of methylcrotonaldehyde to obtain the corresponding allylic alcohols. Meanwhile, the recombinant cells induce the production of D-glucose dehydrogenase, with glucose as a co-substrate, the glucose dehydrogenase can continuously convert NADP+ to NADPH, additional coenzymes do not need to be added in the reaction process, thus the production cost is greatly lowered, and the method is more suitable for large-scale industrial production.
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Description

[0001] (1) Technical field

[0002] The present invention relates to a recombinant cell co-expressing alkenaldehyde reductase and glucose dehydrogenase and its application in the synthesis of prenol.

[0003] (2) Background technology

[0004] α,β-Unsaturated alcohols are very important intermediates in organic synthesis, which are widely used in the production of perfumes, pharmaceuticals and other fine chemicals. Prenol is one of the important α, β-unsaturated alcohols, its scientific name is 3-methyl-2-buten-1-ol, the relative molecular weight is 86.13, and the density is 0.848g·cm -3 , the boiling point is 140℃, the flash point is 43℃, it is a colorless transparent liquid, the solubility in water is 170g·L -1 (20°C). Prenol can be used for: (1) synthesizing the intermediate of high-efficiency and low-toxicity pesticide pyrethroid and its downstream products; (2) synthesizing the intermediate of flavor and fragrance (such as citral); (3) Thiogeraniol, which can be widely ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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