A gene recombinant Saccharomyces cerevisiae with detoxification function and its construction method and application

Abstract

The invention relates to genetic recombination saccharomyces cerevisiae with a detoxification function. The genetic recombination saccharomyces cerevisiae contains an exogenous aldehyde reductase gene, and the exogenous aldehyde reductase gene is an aldehyde reductase gene of which the sequence is shown in SEQ ID NO:1. The exogenous aldehyde reductase gene is obtained in a way that genome DNA of pichia stipitis is taken as a template for PCR amplification, and meanwhile, two enzyme digestion sites including Xho I and Not I are introduced into the terminal C and the terminal N respectively. A GRE2 gene segment is connected to a pYES2 / NTA plasmid vector to obtain a recombinant plasmid pYES2 / NTA-GRE2, and then the recombinant plasmid pYES2 / NTA-GRE2 is transformed into the saccharomyces cerevisiae to obtain the genetic recombination saccharomyces cerevisiae with the detoxification function. The saccharomyces cerevisiae can efficiently ferment pretreatment materials of lignocellulose to produce fuel ethanol; meanwhile, inhibitory or toxic aldehyde substances generated in the fermentation process can be removed, and detoxification of fermentation inhibiting substances in the pretreatmentmaterials of the lignocellulose is achieved, so that it is ensured that the process of producing the ethanol through fermentation of the cellulose is smooth and efficient.

Description

technical field [0001] The invention relates to genetic engineering technology, in particular to a gene recombinant Saccharomyces cerevisiae with detoxification function and its construction method and application. Background technique [0002] With the increasing shortage of petroleum resources and declining reserves, using agricultural straw as raw material to ferment and produce fuel ethanol and various chemical products to meet the needs of human social development is considered to be a clean energy that can replace petroleum. Experts believe that the production of ethanol from lignocellulosic biomass represented by crop straws has good development prospects, and at the same time helps to solve the problem of agricultural production waste disposal, which not only alleviates the resource crisis and food crisis, but also has a great impact on environmental pollution. It is of great significance and provides a guarantee for more sustainable development. [0003] Lignocellu...

AI Technical Summary

Problems solved by technology

However, physical and chemical detoxification methods have their insurmountable shortcomings: a single method cannot remove inhibitors with different physical and chemical properties; the combined use of multiple physical and chemical methods will greatly increase the cost of detoxification, and complex detoxification processes become unfeasible in production ; Some physical and chemical detoxification methods will also produce new pollutants

However, when this strain ferments lignocellulose hydrolyzate, the utilization rate of xylose is very low. The main reason is that various inhibitors in the hydrolyzate inhibit the utilization of xylose by this strain.

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