43 results about "Carboxylic acid reductase" patented technology

The present invention provides methods of enzymatically preparing aldehydes from fatty acids by utilizing a carboxylic acid reductase enzyme to reduce the fatty acids to their corresponding aldehydes. The present invention also provides aldehydes prepared by the methods of the invention.

The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.

A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

The invention belongs to the technical field of building material additive production, and in particular relates to a reagent for inhibiting the shrinkage of cement-based materials. The production method of the reagent comprises the following steps: 1) mixing pomelo peel powder and water into a container to obtain suspension solution, and then add alkaline protease / carboxylate reductase compound enzyme to the container for enzymolysis; 2) after the above enzymolysis is completed, dehydrate and dry to obtain a solid, add a solvent and a catalyst to the solid, and heat it under microwave irradiation Raise the temperature to 85-90°C, heat and irradiate for 20-30min; 3) put the result of step 2) under ultraviolet radiation, add benzophenone and lauryl glucoside, and stir for 10-15min; 4) put the result of step 3) After vacuum stirring the mixture and waste cooking oil for 5-10 minutes, add manganese slag and vacuum stirring to obtain the product.

The invention discloses a method for synthesizing pheromone fatty alcohol acetate in microorganisms based on three intermediate products of fatty acid metabolism pathway as precursor molecules. The reductase CAR, fatty acyl-CoA reductase FAR and aldehyde reductase AHR are used to obtain engineering bacteria that can reduce fatty acyl-ACP, fatty acid or fatty acyl-CoA to produce fatty alcohol, respectively. Finally, alcohol acetyltransferase ATF1 was highly expressed in fatty alcohol-producing engineering bacteria, which was used to catalyze the reaction of fatty alcohol and acetyl-CoA to synthesize pheromone fatty alcohol acetate. The invention can realize the microbial transformation from glucose to fatty alcohol acetate, and provides a feasible way for large-scale biosynthesis of fatty alcohol acetate.