43 results about "Carboxylic acid reductase" patented technology

The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.

The present invention provides methods of enzymatically preparing aldehydes from fatty acids by utilizing a carboxylic acid reductase enzyme to reduce the fatty acids to their corresponding aldehydes. The present invention also provides aldehydes prepared by the methods of the invention.

Process for the production of -lysine by constructing a recombinant microorganism which has a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diamino-pimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.

A method for production of L-lysine is provided which includes the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium contains DNA encoding dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a LysE protein that can enhance the excretion of L-lysine out of the methanol-utilizing bacterium, and the bacterium is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.

A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having α, β-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes.

The invention discloses a method for performing genetic modification on DHDPR (Dihydrodipicolinic Acid Reductase) in corynebacterium glutamicum to increase lysine yield and belongs to the fields of genetic engineering and enzyme engineering. By using the genetic engineering method, DHDPR coding genes dapB in corynebacterium glutamicum JL-6 are subjected to site-specific mutagenesis, and the DHDPRprotein structure is changed, so that the affinity of the DHDPR on different oxidation reduction cofactors is regulated, the defect that the requirement of NADP (H/+) is insufficient in the L-lysine synthesis process is overcome, and the ability of accumulating L-lysine in the strain is improved. According to shaking flask fermentation of recombinant bacteria, the accumulation amount of the L-lysine is 17.6g/L. According to the method disclosed by the invention, the affinity of the oxidation reduction cofactors of the DHDPR in the corynebacterium glutamicum is successfully changed, the defectthat the requirement of the NADP (H/+) is insufficient in the L-lysine synthesis process is overcome, and a new thinking is provided for breeding high-yield L-lysine strains.

The invention relates to a genetically engineered bacterium for synthesizing vanillin. The genetically engineered bacterium comprises a path for synthesizing natural vanillin from vanillic acid: the natural vanillin is generated from the vanillic acid under the catalytic action of carboxylic acid reductase Car and phosphopantetheine transferase Sfp. According to the invention, a carboxylic acid reductase (Car) gene and a phosphopantetheine transferase (Sfp) gene are introduced into a host bacterium to successfully construct a genetically engineered bacterium capable of efficiently catalyzing the reduction of vanillic acid to generate vanillin; meanwhile, the yield of the natural vanillin is further increased in a mode of combining substrate/product tolerance domestication and whole-cell catalysis process optimization regulation. A plurality of repeated experiments show that the highest yield of vanillin produced by catalyzing vanillic acid through a biological method in the currently reported related research can reach 4.05 g/L, and the method has a great industrial application prospect in efficient production of vanillin through the biological method.

The invention provides an engineering bacterium suitable for salicin, which comprises a host and a recombinant plasmid vector, and the recombinant plasmid vector is connected with a gene for expressing and coding glucosyltransferase. The recombinant plasmid vector is also connected with a gene for coding carboxylic acid reductase and phosphoryl transferase, which is co-expressed with the gene for coding glucosyltransferase. The recombinant plasmid vector is also connected with genes for over-expressing and coding shikimic acid kinase, pyruvate kinase, transketolase, 3-deoxy-7-phosphoenanthate synthase, iso-chorismate pyruvate lyase and isochorismate synthase; and the host is also knocked out of genes for coding pykA and pykF, so that the yield of salicin is increased. The invention also provides a construction method and an application of the engineering bacterium for synthesizing salicin. By utilizing the engineering bacteria for synthesizing the salicin, the salicin is produced by using glucose and/or glycerol and other carbon sources, so that efficient biosynthesis of the salicin is realized.

The invention discloses a method for synthesizing pheromone fatty alcohol acetic acid ester in a microorganism, based on a fatty acid metabolic pathway and by using three intermediate products as precursor molecules. The method includes: highly expressing acyl-ACP reductase AAR, carboxylic acid reductase CAR, acyl-CoA reductase FAR and aldehyde reductase AHR in the microorganism to respectively obtain engineering bacteria capable of reducing acyl-ACP, fatty acid or acyl-CoA fat generating alcohol; highly expressing alcohol acetyltransferase ATF1 in the fat-generating alcohol engineering bacteria to catalyze reaction of fatty alcohol and acetyl coenzyme A to synthesize the pheromone fatty alcohol acetic acid ester. By the method, microbial conversion from glucose to the fatty alcohol acetic acid ester can be realized, and a feasible way is provided for biologically synthesizing the fatty alcohol acetic acid ester on a large scale.

This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.

The invention relates to muteins of the pyrroline-5-carboxylate reductase 1 (PYCR1), to nucleic acid molecules comprising a nucleotide sequence encoding such muteins, to methods of determining in a subject a predisposition of having an age related disorder associated with PYCR1, to methods of identifying a compound capable of modifying the expression of PYCR1 and methods of treating a subject having an age-related disorder associated with PYCR1. The invention further relates to a genetically modified animal and a method of modifying the expression of the PYCR1 gene in an animal.

A process for the production of L-pipecolic acid which comprises the step of reducing delta-1-piperideine-6-carboxylic acid by the use of pyrroline-5-carboxylate reductase. The delta-1-piperideine-6-carboxylic acid is obtained by the step of converting L-lysine by the use of lysine 6-aminotransferase encoded by a gene of Flavobacterium lutescens. The steps of reducing delta-1-piperideine-6-carboxylic acid and the converting of L-lysine into L-pipecolic acid by the use of lysine 6-aminotransferase are carried out by using a bacterium transformed with a gene encoding lysine 6-aminotransferase wherein such bacterium comprises pyrroline-5-carboxylate reductase encoded by a gene of Escherichia coli or a coryneform bacterium. A recombinant bacterium which can be used in this production process is also provided. Thus, the present invention can provide an efficient biological process for the production of L-pipecolic acid (or 2-piperidinecarboxylic acid).

The invention discloses a carboxylic acid reductase recombinant plasmid. The recombinant plasmid is obtained by connecting a carboxylic acid reductase gene and aphosphopantetheinyl transferase gene to a pRSFDuet-1 carrier. The invention further provides recombinant bacteria containing the recombinant plasmid, and carboxylic acid reductase SrCAR is further produced by using the recombinant bacteria and is applied to production of 1,2-propylene glycol. According to the present invention, the efficient synthesis approach of the biological preparation of 1,2-propylene glycol is achieved, the application prospect is high, the 1,2-propylene glycol is produced by using the carboxylic acid reductase SrCAR to catalyze glucose, and the yield of 1,2-propylene glycol within 4 h under the condition that the gene of the escherichia coli strain is not knocked out is 4-5 times of the yield of 1,2-propylene glycol synthesized by using a gene knockout escherichia coli strain containing a carboxylic acid reductase MavCAR in literature reports.

The present application relates to a variant polypeptide having attenuated dihydropicolinic acid reductase activity and a method for producing L-threonine using the same.

A method is described for producing an objective substance, for example, an aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an ability to produce the objective substance, wherein the microorganism has been modified to have a specific carboxylic acid reductase (CAR) gene, such as a Gordonia CAR gene, Novosphingobium CAR gene, or Coccomyxa CAR gene.