Process for the production of beta-lysine
A lysine and aspartokinase technology, applied in the field of recombinant microorganisms, can solve the problems of time-consuming and expensive raw materials
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[0060] 1. Cloning of the proximal Clostridium lysine 2,3-aminomutase gene
[0061] According to the conserved regions upstream and downstream of the lysine 2,3-aminomutase genes of Fusobacterium nucleatum and Thermoanaerobacter tengcongensis, a set of oligonucleotide primers were designed for In the isolation of the proximal Clostridium lysine 2,3-aminomutase gene (kamA). Using PCR primers WKJ90 / WKJ65 and WKJ68 / WKJ93, and using the chromosome of Clostridium axeum as a template, the DNA fragments of the upstream and downstream regions, including the N-terminal and C-terminal sequences of the kamA gene, were amplified, respectively. After the sequence analysis of the amplified DNA fragment is completed, it is purified to obtain a product containing the start and end sequences of the kamA structural region. Based on the determined upstream and downstream sequences, PCR primers WKJ105 / WKJ106 were synthesized for isolating the full sequence of the Clostridium proximal kamA gene. ...
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