Process for the production of beta-lysine

Abstract

Process for the production of -lysine by constructing a recombinant microorganism which has a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

Description

technical field

[0001] The present invention relates to a method for producing β-lysine. More specifically, the present invention relates to the use of recombinant microorganisms comprising deregulated forms of DNA molecules necessary for the production of β-lysine. Background technique

[0002] Although β-amino acids are rare compared to the corresponding α-amino acids, they still occur in nature both in free form and in polypeptides. Cardillo and Tomasini, Chem. Soc. Rev. 25:77 (1996); Sewald, Amino Acids 11:397 (1996). Since β-amino acids are stronger bases and weaker acids than the corresponding α-amino acids, polypeptides containing β-amino acids instead of α-amino acids have different backbone atom patterns, leading to new properties.

[0003] In the 1950s, L-β-lysine was identified in several strongly basic peptide antibiotics produced by Streptomyces. Antibiotics that produce L-β-lysine by hydrolysis include puromycin, aflatoxin A, violacein, rosethricin, and dext...

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