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C4 type phosphoenolpyruvate carboxylase (PEPC) gene of corn and application thereof in wheat

A technology of pyruvate carboxylase and phosphoenol, applied in the field of molecular biology, can solve the problem of not exceeding the photosynthetic rate of wheat varieties, and achieve the effect of improving photosynthetic efficiency

Inactive Publication Date: 2014-05-21
HENAN ACAD OF AGRI SCI XIAOMAI INST
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Problems solved by technology

[0007] In terms of wheat, Chen Xuqing et al. (2004) first obtained the transgenic T0 generation wheat with high expression of PEPC enzyme activity, and the net photosynthetic rate increased by 39% compared with the recipient, reaching 19 μmol·CO 2 m -2 ·s -1 Around, but not exceeding, the photosynthetic rate of existing wheat varieties

Method used

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  • C4 type phosphoenolpyruvate carboxylase (PEPC) gene of corn and application thereof in wheat
  • C4 type phosphoenolpyruvate carboxylase (PEPC) gene of corn and application thereof in wheat
  • C4 type phosphoenolpyruvate carboxylase (PEPC) gene of corn and application thereof in wheat

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Embodiment 1

[0022] 1. Cloning of high light efficiency pepc gene and construction of expression vector

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Abstract

The invention discloses a C4 type phosphoenolpyruvate carboxylase (PEPC) gene of corn and an application thereof in wheat. The novel PEPC gene provided by the invention can be stably expressed and inherited in wheat, and the photosynthetic efficiency is remarkably improved compared with that of an acceptor material, thereby providing an important gene source and parent material support for improving the photosynthetic efficiency of C3 crop by means of a C4 type high photosynthetic efficiency gene and breeding high photosynthetic efficiency transgenetic wheat variety with the level of output greatly improved.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a corn C4-type phosphoenolpyruvate carboxylase (PEPC) gene and its application in wheat. Background technique [0002] Wheat is the main food crop in my country, and its production capacity and supply and demand are always related to major strategic issues such as my country's national economic development and food security. From a global perspective, the state of world grain production has always been closely related to the political and economic development of each country. Once grain production and reserves decline, international grain prices will rise sharply, and panic will spread. From a domestic point of view, with the growth of my country's population, the rapid development of industrialization and urbanization, the demand for food will continue to increase. At the same time, since the acreage of grain crops in my country cannot recover significantly, the only way to ensu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/60C12N9/88C12N15/82A01H5/00
Inventor 许为钢王玉民王会伟张磊李艳齐学礼胡琳
Owner HENAN ACAD OF AGRI SCI XIAOMAI INST
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