Genetically engineered bacterium for selectively producing retinol as well as construction method and application thereof

A technology of genetically engineered bacteria and retinol, applied in the fields of metabolic engineering, microorganisms, and genetic engineering, can solve the problem of low selectivity of all-trans isomers, and achieve the effects of increasing yield, good application prospect and yield improvement.
CN113265344AActive Publication Date: 2021-08-17ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2021-08-17

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Abstract

The invention discloses a genetically engineered bacterium for selectively producing retinol as well as a construction method and an application thereof, and belongs to the field of genetic engineering. According to the genetically engineered bacterium, an engineering strain for producing beta-carotene is taken as a starting bacterium, beta-carotene 15, 15'-dioxygenase coding genes and aldehyde reductase coding genes or / and retinol dehydrogenase coding genes are introduced, and selective production of retinol in a cell factory is realized. In order to further improve the yield of retinol, a geranyl-geranyl pyrophosphate synthase mutant encoding gene is further introduced into the genetically engineered bacterium, an NADH kinase encoding gene of an N-terminal mitochondrial positioning peptide is cut off, and meanwhile, a mevalonic acid pathway transcription inhibition factor gene is knocked out, so that the supply of precursors and coenzymes is increased. The invention also provides a method for producing retinol by using the genetically engineered bacterium, ferrous ions and an antioxidant are added in the fermentation process, so that the yield of retinol is further improved.
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Description

technical field

[0001] The invention relates to the fields of genetic engineering, metabolic engineering and microorganisms, in particular to a construction method and application of genetically engineered bacteria capable of realizing selective production of retinol. Background technique

[0002] Vitamin A (Vitamin A, VA) generally refers to substances with similar biological activity to retinol (Retinol), generally composed of 4 consecutive isoprenoid unit side chains and a 20-carbon structure of a β-ionone ring , According to the different groups bonded to the 15th carbon, it can be divided into retinol (hydroxyl group), retinal (aldehyde group), retinoic acid (carboxyl group) and retinyl ester (acyl group). Due to the existence of a special conjugated double bond system, vitamin A is easily oxidized and isomerized leading to its degradation after exposure to light, oxygen and transition metal ions.

[0003] As an essential vitamin for the human body, vitamin A plays an ...

Claims

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