The invention relates to the technical field of biological medicines, in particular to a galangal-sourced nano-
vesicle as well as a preparation method and application thereof. The method specifically comprises the following steps: crushing rhizoma alpiniae officinarum rhizomes, mixing the crushed rhizoma alpiniae officinarum rhizomes with a
buffer solution, soaking in a water bath, and filtering to remove solids so as to obtain a crude extract; and sequentially carrying out centrifugal treatment on the crude extract for four times, so as to obtain the galangal-derived nano-vesicles. The invention provides a method for extracting nano-vesicles from galangal, and finds for the first time that the obtained vesicles can promote osteogenic differentiation of
human bone marrow mesenchymal stem cells with high efficiency and
low toxicity, the effect of the vesicles is obviously superior to that of known
active component galangin, and the
cytotoxicity is obviously lower; the invention provides raw materials and methods with great potential for developing novel and safe anti-
osteoporosis drugs or inducers.