Method for preparing sporo-toxin-bacteria liver-protection vaccine for domestic animal, fowl and aquatic animals
A technology for aquatic animals and poultry, applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, drug combinations, etc. Can not play a health care role and other problems, to achieve the effect of improving the body's immunity and disease resistance, high market promotion and application value, and solving liver and gallbladder abnormalities
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Embodiment 1
[0022] Embodiment one: preparation
[0023] (1) Weigh 10g of hypericum, 10g of astragalus, 5g of pig gall, 5g of Sihuanglian, 5g of licorice, 10g of isatidis, and 8g of galangal, wash and dry (the degree of dehydration is 90%), mix and pulverize, add 4~ Water with 6 times the quality of traditional Chinese medicine was boiled for 2 hours, and the evaporated gas was collected, condensed, and dried to obtain 20 g of concentrated powder of compound Chinese medicine of spore-toxin hepatoprotective vaccine.
[0024] (2) Extract the concentrated powder of the compound traditional Chinese medicine with 100ml 80-90% ethanol solution, let stand at room temperature and wait for stratification, take out the upper layer liquid and carry out centrifugation and drying to obtain 13g of the compound Chinese medicine extract of the spore-forming bacteria hepatoprotective vaccine.
[0025] (3) In a sterile environment, mix 13g of the compound Chinese medicine extract of the spore-toxin liver-pr...
Embodiment 2
[0027] Embodiment two: curative effect
[0028] In the poultry farm, 153 control chickens and 157 test chickens were selected.
[0029] The feed of the test chickens was added with 3% by weight of the spore-toxin hepatoprotective vaccine, and the chickens were fed once a day. After a large outbreak of poultry cholitis, 117 control chickens were infected with cholitis and 36 were healthy; 5 test chickens were infected with cholitis, and 152 were not infected with normal liver, gallbladder and kidney functions, and the prevention rate was 96.8%.
[0030] To 117 control chickens infected with cholitis, adopt spore-toxin hepatoprotective vaccine to treat from the onset period, add 3% mass percentage content of the attenuated bacteria vaccine in the feed, feed three times a day, after three days, 112 recovery from illness and Liver, gallbladder, and kidney functions were all normal, and the cure rate reached 95.7%.
Embodiment 3
[0031] Embodiment three: curative effect
[0032] 103 pregnant sows infected with hepatitis were treated with spore-toxin hepatoprotective vaccine from the onset period, 120ml per day, and fed three times a day. After one week, 99 of them were cured and their liver, gallbladder and kidney functions were normal. Reached 96.1%. The piglets born by the recovered sows through pregnancy are healthy.
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