13 results about "Phytolacca acinosa" patented technology

The invention discloses a phytolacca americana seed EMS mutation library establishment method, and belongs to the technical field of mutation library construction.The phytolacca americana seed EMS mutation library establishment method comprises the steps that concentrated sulfuric acid is used for pretreating phytolacca americana seeds, then germination accelerating is conducted on the seeds, and the seeds are treated with an EMS solution when the seeds are white, so that the problems that phytolacca americana seed coats are thick, and the sensitivity to EMS is low are solved; according to the mutagenesis method disclosed by the invention, high-density series allele point mutation can be induced and generated, the mutagenesis frequency is relatively high, and meanwhile, chromosome distortion is less caused. In the mutagenesis process, abundant genetic variation can be more efficiently created, and more possibilities are provided for screening mutants with excellent characters. Besides, the method has a remarkable advantage in the aspect of cost, the required chemical reagent is cheap, the experimental operation process is simple, and complicated equipment and technology are not required like common irradiation. The breeding cost is reduced, the working efficiency is improved, and wide popularization and application are facilitated.

This invention belongs to the field of traditional Chinese medicine analysis technology, specifically relating to a method for constructing a characteristic chromatogram of *Phytolacca americana*. The method includes preparing a test solution and employing a specific gradient elution procedure under chromatographic conditions to obtain the characteristic chromatogram of *Phytolacca americana*. The characteristic chromatogram of *Phytolacca americana* obtained by the method provided by this invention exhibits good separation of characteristic peaks, clear material basis, and the ability to distinguish different sources of *Phytolacca americana*. It can separate the main chemical components of *Phytolacca americana*, resulting in more accurate, reliable, reproducible, and sensitive results.

The invention relates to the technical field of cutting propagation, and discloses a cutting propagation method for rapid rooting of pokeberry root, which comprises the following steps: S1, taking semi-lignified current-year branches as cutting slips, and soaking the cutting slips in a sterilizing and rooting complexing agent; s2, the treated cutting slips are inserted into a cutting medium; s3, a period of time after cuttage is conducted, fermentation liquor is poured, and the water content of the cuttage medium is controlled in the whole cuttage period. According to the phytolacca acinosa cutting propagation method provided by the invention, the sterilized rooting agent can promote root growth, inhibit bacteria, reduce and improve the stress resistance of cutting slips, and promote base cell activation; and the provided cutting medium ensures the required air permeability and drainage, so that the rooting and survival rate of the pokeberry root are improved.

The invention discloses a method for preventing and treating an invasive plant phytolacca oryzae by using diversity of local plants, and belongs to the technical field of comprehensive treatment of invasive plants. The method for preventing and treating the invasive plant pokeberry root by using the diversity of the local plants comprises the following steps: S1, cutting the overground part of the pokeberry root, and cleaning the underground perennial root system of the pokeberry root; s2, covering the soil surface of the invasion area with a new soil layer which is not invaded by phytolacca petiolata; s3, selecting various common local plant seeds, and sowing the seeds in the area treated in the step S2. The method provided by the invention is simple and clear, and comprises four steps of cutting, crushing, covering and planting, so that the aim of blocking the invasive plant phytolacca pendula by the biological diversity is fulfilled.

The invention relates to an efficient plant genetic transformation vector based on rhizobium rhizogenes, which is modified on the basis of a pTi vector, and a left boundary pTi-LB and a right boundary pTi-RB of T-DNA of the vector are respectively replaced by a left boundary pRi-LB and a right boundary pRi-RB; deleting a Hyg expression cassette in the T-DNA region, and connecting a target gene expression cassette to the front end of the original RUBY expression cassette; a VirGN54D insert element is inserted between an Ori element and an SmR element in a non-T-DNA (Transfer-Deoxyribose Nucleic Acid) region; the 106th site of a RepA protein coding gene sequence in pVS1 of a non-T-DNA region is mutated into a histidine coding sequence from an arginine coding sequence. The invention aims to enhance efficient genetic transformation of plant exogenous genes in multiple species, has broad-spectrum adaptability to plant species, and particularly has obvious effects in multiple plant species which depend on genotypes and are difficult to transform, such as cucumbers, white radishes, broad beans, Chinese little greens, black nightshade, pokeberry roots, gingko, phalaenopsis, Chinese roses, Chinese pines and the like.

The invention discloses a method for creating low-fertility new phytolacca americana germplasm through irradiation mutagenesis and application. Belongs to the technical field of mutation breeding. According to the method disclosed by the invention, the < 60 > Co-gamma rays are used for carrying out irradiation treatment on the phytolacca americana seeds, so that a stable mutant group is successfully constructed. Through multi-generation screening, a low-breeding mutant strain with significantly improved biomass and limited reproductive capacity is obtained. Compared with wild phytolacca americana, the mutant strains show higher biomass and plant height in growth indexes, and show stronger growth vigor; the seed quantity and the pollen quantity are obviously lower than those of the wild type in breeding indexes, so that the ecological invasion risk is effectively reduced. According to the method, the heavy metal enrichment capacity of the phytolacca americana is reserved, a new thought and germplasm resources are provided for application of the phytolacca americana in heavy metal polluted land remediation, and the method has important ecological and environmental significance.

The invention belongs to the technical field of traditional Chinese medicine, and discloses pokeberry root homogeneous polysaccharide PPRP-2-3 which is mainly prepared from rhamnose, arabinose, galactose, glucose and galacturonic acid according to the molar ratio of 10.5: 9.7: 19.9: 9.0: 38.2, and the weight-average molecular weight of the pokeberry root homogeneous polysaccharide PPRP-2-3 is 9.025 KDa. The phytolacca acinosa homogeneous polysaccharide PPRP-2-3 is fixed in component and high in yield, a basis is provided for quality control and standardized production of the phytolacca acinosa homogeneous polysaccharide, and the phytolacca acinosa homogeneous polysaccharide PPRP-2-3 has the effect of treating the non-alcoholic fatty liver disease and is good in safety and free of obvious toxic effect when the concentration is 400 mu g / ml.

The invention discloses application of a nuclear gene ITS sequence in identification and / or distinguishing of pokeberry root and counterfeit products thereof. The 1881st site of the nuclear gene ITS sequence with the GenBank login number of OL824854.1 is T or A, and the 1996 site nucleotide is T or C; or the nucleotide at the 1938th site is G or A, and the nucleotide at the 2031st site is T or A; the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim., the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim. According to the invention, a section of ITS bar code sequence capable of simultaneously identifying Phytolacca latbenia (Moq.) Maxim. In a sample to be detected is found for the first time, and the detection method designed based on the section of sequence is simple and reliable, is not influenced by the characters of medicinal materials, and can be used for accurately identifying medicinal material powder; the method has the advantages of high objectivity, no dependence on subjective judgment and the like.

The application discloses a skin bacteriostatic liquid containing multiple traditional Chinese medicine components and a preparation method and application thereof, relates to the technical field of skin bacteriostatic liquids, and comprises an effective component system and a carrier system; the effective component system is dissolved or uniformly dispersed in the carrier system; according to mass percentage, the effective component system comprises 2% to 4% of Semen Xanthii, 2% to 4% of Rhus galla, 1% to 3% of Pueraria lobata, 0.5% to 2% of Phytolacca acinosa, 0.5% to 2% of Salvia miltiorrhiza and 2% to 3% of a synergistic component; the carrier system is a solvent acceptable in the field of pharmacy or health products; the application takes scientific compounding of multiple traditional Chinese medicines as core effective components, greatly reduces the dependence on strong-irritation chemical bacteriostatic agents, has good skin applicability and a wide bacteriostatic spectrum, can effectively inhibit common bacteria and fungi, and takes into account the bacteriostatic effect and the safety of long-term use; in combination of an optimized formula design and a special stabilization preparation process, the physical and chemical stability of the preparation is effectively improved.

This invention discloses the application of *Phytolacca americana* extract in inhibiting *Fusarium oxysporum* transformant strains and controlling tobacco wilt disease, belonging to the field of plant disease control technology. The *Phytolacca americana* extract is prepared by cold ethanol extraction of the stems, leaves, or roots of *Phytolacca americana*, followed by extraction with ethyl acetate and / or n-butanol. The *Phytolacca americana* extract shows an inhibition rate of up to 57.47% against *Fusarium oxysporum* transformant strains. In vitro toxicity tests and pot experiments show that the extract has significant inhibitory and control effects on *Fusarium oxysporum* transformant strains and tobacco wilt disease at a concentration of 1.805 mg / mL. Both foliar spraying and root irrigation methods achieve good disease prevention and treatment effects. The *Phytolacca americana* extract is derived from plants, is environmentally friendly, and is less likely to induce drug resistance in pathogens, providing a new, highly effective, and environmentally friendly agent for the control of tobacco wilt disease, with broad application prospects.

The present application relates to the technical field of external medicine for treating urticaria, and discloses a composition for treating urticaria, which comprises the following components: Cudrania tricuspidata, Eucalyptus globulus, Cymbopogon citratus, Datura stramonium, Kaempferia rotunda, Sageretia theezans, Gleditsia sinensis, camphor, Gnaphalium affine and Gynura japonica. The composition is externally applied to the skin of the affected part, and has the effects of clearing heat and detoxifying, removing dampness and swelling, dispelling wind and relieving itching.

The invention belongs to the field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine composition patch for adjuvant treatment of chronic heart failure and a preparation method thereof. The traditional Chinese medicine composition patch is prepared from radix aconiti lateralis preparata, radix phytolaccae, semen lepidii and rhizoma chuanxiong. The traditional Chinese medicine composition patch is prepared from the medicine and an adhesive in proportion, the bioavailability of the medicine is high, the components are clear, and the treatment effect is good. And by controlling the specific types and proportions of the effective components of the medicines, the mutual synergistic effect of the components is improved, and the effect of treating the chronic heart failure with reduced ejection fraction is improved. The components of the medicine are reasonably matched, and the clinical symptoms and life quality of chronic heart failure patients with reduced ejection fraction can be remarkably improved by acting on the Shenque acupoint.

The invention relates to application of saponin in inhibition of penicillium. The saponin is at least one of oleanolic acid type saponin, hedera helix type saponin and phytolaccae acid type saponin. On the basis, the citrus preservative can be used for citrus preservation to improve the quality and prolong the shelf life of citrus so as to guarantee the commodity value of fruits.