33 results about "Coumestrol" patented technology

A composition having phytoestrogenic and anti-cancer activity is described. The composition comprises wogonin, isoliquiritigenin, coumestrol, their pharmaceutically acceptable salts or esters, their selectively substituted analogs, or combinations thereof. The compositions may also include an anti-cancer agent and / or an immune stimulant. A method for treating or preventing cancer or an estrogen-related disorder includes administering a therapeutically effective amount of the compositions is described. The compositions are particularly useful in the treatment of hormone-related cancers.

A composition having phytoestrogenic and anti-cancer activity is described. The composition comprises wogonin, isoliquiritigenin, coumestrol, their pharmaceutically acceptable salts or esters, their selectively substituted analogs, or combinations thereof. The compositions may also include an anti-cancer agent and / or an immune stimulant. A method for treating or preventing cancer or an estrogen related disorder includes administering a therapeutically effective amount of the compositions is described. The compositions are particularly useful in the treatment of hormone-related cancers.

The present invention relates to a cosmetic composition for skin care, comprising coumestrol or a bean extract containing coumestrol.

The present invention relates to a skin cosmetic product containing 3,9-diferulylcoumestrol, especially to a skin cosmetic product containing a compound in which ferulylic acid is bonded to a coumestrol. The skin cosmetic product has several excellent effects for improving cosmetic disorders, such as anti-aging effect, whitening effect and hair-seeding effect.

The present invention relates to a composition for activating mitochondria comprising coumestrol or a bean extract containing coumestrol.

A new immunosuppressive agent as glycyrol has significant immunosuppressive activity, thus is useful as an immunosuppressive agent to treat relative diseases, wherein the glycyrol is a coumestrol compound extracted from glycyrrhiza, which is a small molecular compound with novel chemical structure. Tests prove that the glycyrol can inhibit the increment of T lymphocyte induced by canavaline A and inhibit one-way mixed lymphocyte reaction (MLR), release the inflammatory symptom of delayed hypersensitivity and inhibit the homologous allogenic organ transplantation rejection. The glycyrol is a high-effective low-toxicity immunosuppressive agent, with small molecular weight and simple structure, which is easy to be modified and to prepare different drug agents, therefore, the compound is a new generation immunosuppressive agent.

The present invention provides new coumestans compounds and processes for the preparation of coumestans, pharmaceutical compositions having a coumestan as an active pharmaceutical ingredient, and methods of utilizing coumestans as selective estrogen receptor modulators (SERMs) for treating estrogen dependent diseases such as breast cancer.

The present invention provides gero-protective pharmaceutical compositions and methods, the compositions being adapted to enhance at least one of cell survival and cell metabolism in a mammalian subject, the pharmaceutical compositions include at least two of Withaferin a or its structural analogs, coumestrol, ginsenoside, quinidine, silymarin, licochalcone a, lipoic acid and apigenin, in a pharmaceutically effective amount.

The invention relates to the technical field of breeding of small yellow croakers, in particular to a feminization inducing method for small yellow croakers with undifferentiated sex glands. The method comprises the following steps: placing fry of small yellow croakers hatched for 13-15 days and having undifferentiated sex glands in seawater containing estrogen with a low concentration of 1-10 [mu]g / L for dipping bath, and performing dipping bath for 2 hours every day and continuous for 60 days, so as to enable the crowd of small yellow croakers to be completely feminized, wherein the selectable estrogen comprises estrone, soybean isoflavone, estradiol, estriol, coumestrol and genistein. The effect of estradiol is more obvious, 1 [mu]g / L estradiol can ferminize 96.6% of a crowd of small yellow croakers, 10 [mu]g / L estradiol can ferminize 100% of a crowd of small yellow croakers, the induction rate is high, the operatability is high, natural environment does not cause limit. Female parent fish guarantee is provided for large-scale artificial breeding of small yellow croakers, a technological route is opened up for study on sex control and monosex breeding of small yellow croakers, and a route for solving the problem of less of female population of small yellow croakers is provided.

The invention discloses a composition for activating mitochondria. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of coumestrol, 5-10 parts of coenzyme Q10, 10-20 parts of ellagic acid, 1-3 parts of quercetin, 5-10 parts of a grape seed extract, 10-15 parts of a silybum marianum extract, 10-15 parts of an olive leaf extract, 10-15 parts of borage, 3-6 parts of nostoc sphaeroids kutz ferment powder, 4-8 parts of broken-down wall ganoderma lucidum spore powder, 10-15 parts of acetylcarnitine and 5-10 parts of superfine grinding powder of pecan bark. According to the obtained composition, the activity of mitochondria enzyme can be improved and the quantity of mitochondria DNAs can be increased, so that the composition is capable of activating the mitochondria and increasing biosynthesis of the mitochondria. Therefore, the composition can be effectively used for preventing or treating various mitochondria activity reduction-related diseases, such as degenerative diseases and parkinson's disease.

The present invention relates to a composition for activating mitochondria comprising coumestrol or a bean extract containing coumestrol.

The present invention relates to a skin cosmetic product containing 3,9-diferulylcoumestrol, especially to a skin cosmetic product containing a compound in which ferulylic acid is bonded to a coumestrol. The skin cosmetic product has several excellent effects for improving cosmetic disorders, such as anti-aging effect, whitening effect and hair-seeding effect.

The invention discloses a synthetic method of a polyhydroxy substituted coumestrol natural product. 2-bromine-4-hydroxyphenylacetic acid serves as a raw material to make a condensation reaction with a hydroxy substituted salicylaldehyde compound, and a hydroxy substituted 3-(2-bromine-4-hydroxycyclohexyl phenyl)-7-hydroxy coumarin compound is obtained; and then a hydroxylation / oxidative coupling reaction is made under the condition of a catalyst and microwave heating, and the polyhydroxy substituted coumestrol natural product is obtained. The synthetic method is simple in route, easy and convenient to operate, high in yield, soft in reaction condition, free of needs of precious metal catalyzing, high in atom economy and low in cost, and multiple steps of cascade reactions are made simultaneously.

The invention discloses an anti-aging repair product for a female reproductive system and a preparation method of the anti-aging repair product. Main components of the anti-aging repair product comprise coumestrol, viatmin E, rhizoma curcumae, sodium hyaluronate and collagen. The anti-aging repair product disclosed by the invention can improve the estrogen level in a body when estrogen is insufficiently secreted and also can promote the expression of an estrogen receptor on reproductive system tissue cells of the reproductive system; the estrogen is combined with the estrogen receptor on vaginal tissues to improve lycogenesis, so that lactobacillus favorably degrades glycogen to produce lactic acid for keeping the vaginal acid environment; pathogenic bacteria are resisted, and senile vaginitis is prevented; meanwhile, vaginal lubrication degree and wettability can be improved, and vaginal dryness and atrophy are avoided; besides, degenerative change of the female reproductive system isprevented or improved in a safe effective mode.

Provided are a composition including coumestrol extracted from soybeans as an active ingredient, and a method of inhibiting cortisone reductase by applying it to skin of a subject.

The invention discloses a nonsense mutation read-through function of indole 3 methanol. Preliminary experimental results show that coumestrol can partially recover expression of luciferase containingnonsense mutation in a cell model, and can partially recover expression of ATM genes containing nonsense mutation in an extracellular protein truncation experiment, and the coumestrol is expected to serve as a lead compound, is developed into a new drug through structural modification and modification, treats various diseases caused by nonsense mutation, and has potential medical application.

The present invention relates to an increasing method for increasing the content of coumestrol of germinated and fermented beans by germinating and fermenting beans and then irradiating visible lightthereto by means of light emitting diodes, and germinated and fermented beans produced thereby.

The invention discloses a synthetic method of a polyhydroxy substituted coumestrol natural product. 2-bromine-4-hydroxyphenylacetic acid serves as a raw material to make a condensation reaction with a hydroxy substituted salicylaldehyde compound, and a hydroxy substituted 3-(2-bromine-4-hydroxycyclohexyl phenyl)-7-hydroxy coumarin compound is obtained; and then a hydroxylation / oxidative coupling reaction is made under the condition of a catalyst and microwave heating, and the polyhydroxy substituted coumestrol natural product is obtained. The synthetic method is simple in route, easy and convenient to operate, high in yield, soft in reaction condition, free of needs of precious metal catalyzing, high in atom economy and low in cost, and multiple steps of cascade reactions are made simultaneously.

The present invention relates to a germinated fermented soybean extract containing coumestrol, a preparation method therefor, and a composition containing the germinated fermented soybean extract. Itcan be seen that the germinated fermented soybean extract of the present invention is effective in the hepatocyte protection against hepatotoxicity through in vitro antioxidative activity and cell viability, and hematological tests and MDA, GST, GSH, SOD, and CAT measurements in animal models. In addition, the germinated fermented soybean extract of the present invention increases the mineral content in bones, thereby significantly increasing the bone density, and has an effect of increasing the thickness and number of spongy bones. Furthermore, the germinated fermented soybean extract of thepresent invention has an effect of relieving symptoms of menopause.

The present invention relates to a method for increasing the coumestrol content of germinated fermented beans by irradiating visible light with a light-emitting diode after germinating and fermenting beans, and germinated fermented beans produced by the method.

The invention relates to the field of pharmaceutical chemistry, in particular to a coumestrol open-ring analogue with structures as shown in general formulae I and II and a medical application thereof, especially an application to the preparation of drugs as angiogenesis inhibition and vascular disruption agents.

The invention relates to an in-vitro amplification method of cord blood Treg cells, comprising the steps of S1, isolating mononuclear cells; S2, adjusting cellular density, inoculating to a culture flask, and adding inactivated plasma; on the first day, adding coumestrol, TGF-beta, CD3 / CD28 monoclonal antibody and IL-2; S3, on the fourth day, supplementing a culture medium, adding inactivated plasma, coumestrol and TGF-beta, and keeping the final concentration constant; S4, on the sixth day, supplementing the culture medium, and keeping the final concentrations of other components unchanged; S5, on the ninth day, supplementing the culture medium, and keeping the final concentrations of other components unchanged; S6, on the twelfth day, collecting cells. The amplification of CD4, CD25 and Tregs is induced under the coaction of coumestrol and TGF-beta, the amplifying efficiency is high, and high-purity Treg cells can be acquired without pre-purification.

The present specification relates to a culturing method for cultured leguminous roots having an increased coumestrol content, the method being capable of mass-producing coumestrol, which is present in a very small amount in a leguminous plant, wherein the culturing method comprises the steps of: (a) germinating leguminous seeds in a culture medium to induce in vitro plants having cotyledons, hypocotyls, and radicles; (b) culturing, in a culture medium, at least one site of cotyledons, hypocotyls, and radicles of the induced in vitro plant to induce site-specific cultured roots; and (c) multiplying the induced site-specific cultured roots in a culture medium, wherein the culture medium contains nutrient components of NH4NO3, CaCl2.2H2O, MgSO4.7H2O, KH2PO4, and KNO3.

The present invention relates to a germinated and fermented bean extract containing coumestrol, a preparation method thereof, and a composition comprising the germinated and fermented bean extract. For liver toxicity, the germinated and fermented bean extract of the present invention effectively protects the liver through the in vitro (in vitro) antioxidant activity and cell survival rate and blood tests of animal models, measurement of MDA, GST, GSH, SOD, CAT, etc. cell. In addition, it significantly increases bone density by increasing bone mineral content and has the effect of increasing the thickness and amount of cancellous bone. In addition, it also has the effect of relieving menopausal symptoms.

The invention discloses a nonsense mutation read-through function of coumestrol. The primary experiment result shows that expression of luciferase containing nonsense mutation can be partially recovered by the coumestrol in a cell model, in the extracellular protein truncation experiment, expression of an ATM gene containing nonsense mutation can be partially recovered by the cumestrol, and the coumestrol is expected to serve as a lead compound for developing new medicine through structural transformation and modification to treat various diseases caused by nonsense mutation, and the coumestrol has potential medicinal application.

The specification relates to a method for producing coumestrol by using cultured roots of leguminous plants and a method for preparing a leguminous plant extract having an increased coumestrol amount.A method according to one aspect of the present invention enables a large amount of cumestrol-to-be-produced to be uniformly obtained by using leguminous plants and enzymes so as to save more costs and time than a conventional method, and can be safely used in various fields, such as in pharmaceuticals, foods or cosmetics since cumestrol is produced using a natural material.

The present invention provides gero-protective pharmaceutical compositions and methods, the compositions being adapted to enhance at least one of cell survival and cell metabolism in a mammalian subject, the pharmaceutical compositions include at least two of Withaferin a or its structural analogs, coumestrol, ginsenoside, quinidine, silymarin, licochalcone a, lipoic acid and apigenin, in a pharmaceutically effective amount.

The present invention relates to a composition for, inter alia, preventing or treating a climacteric disease, or preventing or improving a climacteric skin symptom comprising a soybean extract comprising coumestrol as an active ingredient, and more specifically relates to a composition for, inter alia, preventing or treating a climacteric disease, or preventing or improving a climacteric skin symptom comprising a germinated and fermented soybean extract comprising coumestrol as an active ingredient.