Feminization inducing method for small yellow croakers with undifferentiated sex glands

An undifferentiated, small yellow croaker technology, applied in fish farming, climate change adaptation, application, etc., can solve the problem of low proportion of female broodstock, and achieve obvious effects, good plasticity, lasting and irreversible effects.

Inactive Publication Date: 2018-05-29
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the low proportion of female broodstock in artificially propagated small yellow croakers, the object of the present invention is to provide a method for inducing feminization of undifferentiated gonad small yellow croakers, inducing feminization of undifferentiated gonad small yellow croakers to obtain a high p...

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  • Feminization inducing method for small yellow croakers with undifferentiated sex glands
  • Feminization inducing method for small yellow croakers with undifferentiated sex glands

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Effect test

Embodiment 1

[0030] A method for inducing feminization of gonadal undifferentiated small yellow croaker, characterized in that it comprises the following steps:

[0031] (1) Weigh 0.25 g of estradiol and dissolve it in 95% ethanol solution to obtain an estradiol solution with a concentration of 1 mg / ml;

[0032] (2) Add 350L of seawater to a culture tank with a volume of 500L. The seawater temperature is 18°C ​​and the salinity of seawater is 28‰, and then aerate the culture tank to maintain sufficient oxygen content;

[0033] (3) 300 gonadal undifferentiated small yellow croaker juveniles were placed in the culture tank of step (2), wherein the selected gonadal undifferentiated small yellow croaker juveniles were 13-day-old small yellow croaker juveniles after hatching;

[0034] (4) Add 0.35mL of estradiol solution to the breeding tank so that the concentration of estradiol in seawater is 1µg / L, and keep the juvenile small yellow croaker in the seawater containing estradiol for 2 hours; ...

Embodiment 2

[0038] A method for inducing feminization of gonadal undifferentiated small yellow croaker, characterized in that it comprises the following steps:

[0039] (1) Weigh 0.25 g of estradiol and dissolve it in 95% ethanol solution to obtain an estradiol solution with a concentration of 1 mg / ml;

[0040] (2) Add 400L of seawater to a culture tank with a volume of 500L. The seawater temperature is 23°C and the salinity of seawater is 30‰, and then aerate the culture tank to maintain sufficient oxygen content;

[0041] (3) Put 400 undifferentiated gonadal juveniles of small yellow croaker in the culture tank of step (2), wherein the selected gonadal undifferentiated small yellow croaker juveniles are 15-day-old small yellow croaker juveniles after hatching;

[0042] (4) Add 4 mL of estradiol solution to the breeding tank so that the concentration of estradiol in seawater is 10 µg / L, and keep the juvenile small yellow croaker in the seawater containing estradiol for 2 hours;

[0043]...

Embodiment 3

[0046] A method for inducing feminization of gonadal undifferentiated small yellow croaker, characterized in that it comprises the following steps:

[0047] (1) Weigh 0.25 g of estradiol and dissolve it in 95% ethanol solution to obtain an estradiol solution with a concentration of 1 mg / ml;

[0048] (2) Add 450L of seawater to a culture tank with a volume of 500L. The seawater temperature is 28°C and the salinity of seawater is 31‰, and then aerate the culture tank to maintain sufficient oxygen content;

[0049] (3) 500 undifferentiated gonadal juveniles of small yellow croaker were placed in the culture tank of step (2), wherein the selected gonadal undifferentiated small yellow croaker juveniles were 14-day-old small yellow croaker juveniles after hatching;

[0050] (4) Add 10.5mL of estradiol solution to the breeding tank so that the concentration of estradiol in seawater is 30µg / L, and keep the juvenile small yellow croaker in the seawater containing estradiol for 2 hours; ...

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Abstract

The invention relates to the technical field of breeding of small yellow croakers, in particular to a feminization inducing method for small yellow croakers with undifferentiated sex glands. The method comprises the following steps: placing fry of small yellow croakers hatched for 13-15 days and having undifferentiated sex glands in seawater containing estrogen with a low concentration of 1-10 [mu]g/L for dipping bath, and performing dipping bath for 2 hours every day and continuous for 60 days, so as to enable the crowd of small yellow croakers to be completely feminized, wherein the selectable estrogen comprises estrone, soybean isoflavone, estradiol, estriol, coumestrol and genistein. The effect of estradiol is more obvious, 1 [mu]g/L estradiol can ferminize 96.6% of a crowd of small yellow croakers, 10 [mu]g/L estradiol can ferminize 100% of a crowd of small yellow croakers, the induction rate is high, the operatability is high, natural environment does not cause limit. Female parent fish guarantee is provided for large-scale artificial breeding of small yellow croakers, a technological route is opened up for study on sex control and monosex breeding of small yellow croakers, and a route for solving the problem of less of female population of small yellow croakers is provided.

Description

technical field [0001] The invention relates to the technical field of small yellow croaker breeding, in particular to a method for inducing feminization of undifferentiated gonad small yellow croaker. Background technique [0002] Small yellow croaker ( Larimichthys polyactis ) is an important economic fish in my country's "Four Seafoods". Due to overfishing, environmental pollution and climate change, the captured small yellow croakers are increasingly miniaturized, and the germplasm resources are seriously degraded. The catch of small yellow croakers has dropped sharply, which can no longer meet people's consumption needs. A preliminary study on the fish population structure and reproductive characteristics of small yellow croakers in the southern Yellow Sea found that the natural population of small yellow croakers has a male-to-female ratio of about 1:3, and found that the sexual maturity of small yellow croakers is earlier, generally 1 year old. In 2015, the Zhejiang...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 谢庆平楼宝詹炜陈睿毅刘峰何雪王立改徐冬冬
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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