61 results about "Larimichthys polyactis" patented technology

The invention provides a little yellow croaker female fish and large yellow croaker male fish crossbreeding method, and belongs to the technical field of aquaculture seawater fish larval breeding. The method comprises the following steps of 1, parent selection and culture; 2, artificial hasten parturition and insemination: a, artificial hasten parturition; b, artificial egg extrusion and semen collection; c, artificial dry process and insemination; d, egg washing; 3, larval breeding management; 4, larva and young fish culture. The method has the advantages that the little yellow croaker and large yellow croaker crossbreeding is performed; new varieties with high growth speed and excellent quality can be cultured; in addition, the fertility rate of the new varieties can reach 65 percent or higher; the hatching rate reaches 92 percent or higher; important application values are realized on the production; the health development of the aquaculture industry in China is promoted.

The invention discloses calcium supplementing piglet feed, which is prepared from the following ingredients in parts by weight: 120 to 140 parts of soybean meal, 6 to 9 parts of fresh little yellow croakers, 10 to 15 parts of kelp, 10 to 17 parts of dried small shrimps, 7 to 13 parts of animal bone meal, 3 to 5 parts of spina date seed, 5 to 7 parts of dry vinasse, 2 to 3 parts of Itoa, 2 to 4 parts of roughhaired holly root, 6 to 8 parts of hulled oat, 1 to 2 parts of angelica powder, 10 to 13 parts of chenopodium album, 3 to 5 parts of negundo chastetree leaves, 4 to 6 parts of corn stigma and 5 to 7 parts of nutrition flavoring powder. The feed provided by the invention has the advantages that the calcium requirements of piglets can be met, and the piglet growth vigor can be improved, wherein the fresh little yellow croakers, the dried small shrimps and the animal bone meal are subjected to heat treatment, the bone can be limp, the calcium can be more conveniently digested and absorbed, the piglet immunity is improved, and the piglet health is promoted.

The invention relates to a primer sequence and a method for rapid identification of pseudosciaena crocea, larimichthys polyactis and collichthys lucidus. The primer sequence is as follows: 1F:5'-GGAAAGAGCCAGGAAAGC-3' and 1R:5'-GGCGGAACTCTGAGCAAA-3'. The method includes the steps of (1) extracting individual genomic DNA; (2) synthesizing a specific primer for sequence amplification and performing PCR (Polymerase Chain Reaction) amplification; and (3) detecting the PCR product through agarose gel electrophoresis, taking pictures and performing recording, obtaining respective specific DNA fragment spectrograms of the three kinds of fishes according to different migration distance of the amplification products, and performing distinguishing in accordance to attached spectrograms. The method can simply and accurately distinguish individual samples of the three kinds of fishes, and provides a new fast research method for germplasm identification of the three kinds of economic fishes with similar appearance.

The invention discloses a method for making instant little yellow croaker. The method comprises the following steps: 1) pretreatment; 2) fishiness removal and salting; 3) rinsing; 4) soaking; 5) steaming; 6) coating; 7) frying; and 8) vacuum packing. The taste in dipping juice enters the meat by dipping; the original fresh taste of fish is reserved and the fragrance of lotus leaves is left in the fish in the lotus leaf wrapping and steaming process, so that flavoring and greasiness removing effects are achieved; the coating process prevents the meat of the little yellow croaker from scattering in the frying process and ensures that the product is parched outside and tender inside after drying; crushed spearmint, cinnamon leaves and fennel are combined with garlic, so that the product is more delicious; meanwhile, the product is golden yellow in appearance and interspersed with jade green, so that the appetite is stimulated; the instant little yellow croaker prepared by adopting the method has good mouthfeel and long shelf life, is safe in eating in the absence of preservatives, sanitary and convenient to eat and carry; and the making method is simple, reasonable and strong in operability, and easily realizes industrialized production.

The invention provides a primer and method for identifying germplasm of large yellow croakers and small yellow croakers. The invention is characterized in that the primer sequences are as follows: F1: 5'-TCGGCCTCCTGGGATTTATTGTC-3'; F2: 5'-ATTATCCTGACGGGTACGCTCTAT-3', R1: 5'-ACACGCGGGGGTCTAAC-3'; and R2: 5'-ATATGCATCGGGGTAATCTGAGTA-3'. The method comprises the following steps: adopting a phenol-chloroform extraction method to extract the DNA in the samples of the large yellow croakers or small yellow croakers; carrying out polymerase chain reaction (PCR): the PCR system is 25mu L, various components include Buffer, dNTP, Mg2+, four primers, Taq and suspected DNA in the samples of the large yellow croakers or small yellow croakers, and double distilled water is used for filling to 25mu L; and detecting the product by agarose gel electrophoresis after PCR, observing, photographing and recording the result and comparing the electrophoresis result with the provided standard electrophoretogram to identify the germplasm. The method is quick and accurate.

The invention discloses a method for preparing a functional minced small yellow croaker product by utilizing mixed-bacterium fermentation. The method comprises the main steps: monascus and lactobacillus are added to minced small yellow croakers which are cut and mixed, and the functional minced small yellow croaker product is prepared through joint fermentation. The functional minced small yellow croaker product prepared through joint fermentation of the monascus and the lactobacillus has a good flavor and gel properties, and the problems of heavy fishy smell, poor flavor and the like of an existing small minced yellow croaker product are solved. Food microorganisms applicable during fish product processing can be extended, a novel minced fish product is also developed, and the technological level of aquatic product processing and added value of the product can be increased.

The invention discloses a high-survival-rate propagation method of larimichthys polyactis fries in low-salinity sea areas and belongs to the technical field of seawater fish fry rearing. The method ischaracterized by including following steps: 1), adjusting salinity of a spawning pond; 2), collecting fertilized eggs; 3), separating high-quality fertilized eggs; 4), hatching the fertilized eggs; 5), desalting breeding water, and managing the fries. By the method, impact on embryonic development even death during embryonic development caused by the fact that the high-quality fertilized eggs inthe spawning pond sink to the bottom due to low water salinity can be avoided effectively, and easy collection of the high-quality fertilized eggs is facilitated. By lowering salinity of a nursery pond at a specific rate, normal growth and development of newly-hatched fries can be realized with lowest impact, and highest survival rate is ensured.

The invention relates to the technical field of mariculture fish propagation and breeding, and particularly relates to an early propagation and rapid breeding method of little yellow croakers. The method includes preparation of a parent fish pond and an incubation cylinder, selection of parent fish to nutrient enhancement, ovulation and fertilization, oosperm incubation, breeding cultivation and others. Through the early propagation and rapid breeding method of little yellow croakers, the spawning fertilization time of the little yellow croakers is advanced by 1 month in comparison to nature condition, the rate of inducing spawning is 88.2+/-0.2%, rate of fertilization is 78.4+/-0.1%, and the hatching rate is 76.7+/-0.1%; the fry breeding time of small yellow croaker with body length of 5cm is shortened by 50 days in comparison to that under the nature condition; the average body length of fry at the beginning of June is up to 7.8 cm, the survival rate is improved by 20%; thus the early emergency and advanced marketing of the little yellow croakers are realized; the offspring seeds of the little yellow croakers are high in price and good in economic benefit; meanwhile, the market demand on large-sized little yellow croakers can be released, and a way for protecting the fishing resource of the little yellow croakers in the East China Sea is provided.

The invention discloses a PCR (polymerase chain reaction) identification method for young little yellow croakers and large yellow croakers and a primer. The PCR identification method comprises the following steps: extracting genome DNA of a sample, performing PCR amplification on the extracted DNA with the identification primer, and performing agarose gel electrophoresis detection on the PCR amplification product, wherein the sample is a little yellow croaker when one amplification strip occurs and is a large yellow croaker when two amplification strips occur. The identification method disclosed by the invention is simple and easy to implement and high in specificity, and can clearly identify and distinguish plesiomorphous young little yellow croakers and large yellow croakers.

The invention relates to the technical field of breeding of small yellow croakers, in particular to a feminization inducing method for small yellow croakers with undifferentiated sex glands. The method comprises the following steps: placing fry of small yellow croakers hatched for 13-15 days and having undifferentiated sex glands in seawater containing estrogen with a low concentration of 1-10 [mu]g/L for dipping bath, and performing dipping bath for 2 hours every day and continuous for 60 days, so as to enable the crowd of small yellow croakers to be completely feminized, wherein the selectable estrogen comprises estrone, soybean isoflavone, estradiol, estriol, coumestrol and genistein. The effect of estradiol is more obvious, 1 [mu]g/L estradiol can ferminize 96.6% of a crowd of small yellow croakers, 10 [mu]g/L estradiol can ferminize 100% of a crowd of small yellow croakers, the induction rate is high, the operatability is high, natural environment does not cause limit. Female parent fish guarantee is provided for large-scale artificial breeding of small yellow croakers, a technological route is opened up for study on sex control and monosex breeding of small yellow croakers, and a route for solving the problem of less of female population of small yellow croakers is provided.

The invention relates to a primer and a method for rapidly distinguishing larimichthys polyactis and larimichthys crocea and determining a hybrid variety of the larimichthys polyactis and the larimichthys crocea, which belong to the field of identification of molecular markers. A nucleotide sequence of an upstream primer of the primer is shown as SEQ ID No. 1, and a nucleotide sequence of a downstream primer is shown as SEQ ID No. 2. By adopting the primer and the method, complex steps such as sequence measurement are not needed, after the appropriate PCR proliferation, a hybrid filial generation can be accurately determined as the hybrid variety, and the larimichthys polyactis and hybrid fish can be distinguished, so that a novel simple and rapid identification method is provided for identifying the germplasm of three types of fish with similar appearance.

The invention provides a pseudosciaena polyactis fresh-keeping method. The pseudosciaena polyactis fresh-keeping method comprises following steps: 1, acid solution immersion; 2, composite fresh-keeping solution immersion; 3, film forming; and 4, packaging and refrigeration. The beneficial effects are that: bamboo vinegar and the composite fresh-keeping solution are used for immersion bacteriostasis, no antibiotic or conventional bactericide is added, the pseudosciaena polyactis fresh-keeping method is safe, and is friendly to the environment; characteristic putrefying bacteria in pseudosciaena polyactis can be inhibited obviously, degradation speed of proteins in pseudosciaena polyactis meat is slowed down, fresh keeping degree is improved, and nutrient loss is reduced; oxidation resistance is achieved, oxidative rancidity of grease in pseudosciaena polyactis preservation process is inhibited, and the preservation period and self-life of pseudosciaena polyactis is prolonged; at the same time, sodium alginate polysaccharide gel and metal cations possess film forming performance, so that decoloring and water loss in pseudosciaena polyactis fresh-keeping process are delayed, the mouthfeel close to that of fresh pseudosciaena polyactis is maintained; operation is simple, cost is low, and market application prospect is promising.

The invention discloses a hybrid breeding method of frozen sperms of small yellow croaker and large yellow croaker, and belongs to the technical field of fry breeding of aquaculture marine fish. The method specifically comprises the following steps that small yellow croaker sperms are collected, a sperm dilute containing an anti-freezing agent is added in the small yellow croaker sperms, cryopreservation is conducted, large yellow croaker ovums are collected, the small yellow croaker sperms are thawed, and the sperms and the ovums are subjected to artificial insemination. According to the method, the sperms with the survival rate being 60% after thawing are selected to be crossed with the large yellow croaker ovums, the fertilization rate reaches 67.3%, and the hatching rate is 86.8% or above. The method has the important significance for overcoming reproductive isolation, achieving artificial hybridization, cultivating new varieties, and achieving large-scale fry breeding.

The invention discloses a larimichthys polyactis microsatellite DNA molecular marker. The invention adopts the technical scheme that an enhanced library of larimichthys polyactis microsatellites (GT) 17, (GGT) 9 and (CTAT) 8 is built, larimichthys polyactis polymorphic microsatellite primers are screened, and the genetic polymorphism detection is carried out on larimichthys polyactis microsatellite loca, so that 21 larimichthys polyactis microsatellite markers with rich polymorphism are developed. The larimichthys polyactis microsatellite DNA molecular marker is applicable to researches on the population genetic structure and the genetic variation level of a larimichthys polyactis population.

The invention discloses soy-sauce-flavor-type pickling fluid for small yellow croakers. A formula of the soy-sauce-flavor-type pickling fluid consists of the following raw materials of water, a Chinese prickly ash solution, cooking wine and salt. The invention further discloses a soy-sauce-flavor-type pickling method for the small yellow croakers. The soy-sauce-flavor-type pickling method comprises the following steps of performing selection, removing scales, making slits at the back, performing cleaning to remove watery blood, performing pickling, performing low-temperature preservation and the like. A great quantity of research experiment proves that the pickling fluid disclosed by the invention can produce unique soy-sauce-flavor taste, naturally give out fragrant and hot smell, and meet the taste sense enjoyment requirements of people; and when the small yellow croakers are pickled with the pickling fluid, nutrient components can be maintained, and the quality guarantee period of the pickled small yellow croakers at a cold chamber of minus 18 DEG C can be prolonged to 1 year.

The invention discloses a fish posture recognition method, and belongs to the field of image processing. The method comprises the following steps: rotating a preprocessed fish image to enable a fish body to be in a horizontal state in the image; cutting the fish in the rotated image, and zooming the cut fish to a set size; making corresponding labels as a training set and a test set according to the fish postures in the images, and training a convolutional neural network to obtain a fish posture recognition model; and performing posture recognition on the fish in the input image by using the fish posture recognition model. According to the invention, the convolutional neural network is adopted to identify the posture of the small and long fish, and when a training set is made, posture correction, cutting and scaling and other operations are performed on the fish image, so that the method can well adapt to the morphological characteristics of the small and long fish (such as pneumatophorus japonicus and little yellow croaker), and the automatic early processing of a fish processing production line is realized; the defects of long time consumption and large workload of the traditional manual processing are overcome, and the invention has the advantages of rapidness, accuracy and high efficiency.

The invention discloses a preparation method and device for desalted larvae of small yellow croakers, and the method comprises the following steps: 1, selection of a culture pond: selecting the culture pond according to the water amount and the quantity of the larvae of the small yellow croakers, and adding natural seawater and the larvae of the small yellow croakers into the culture pond; 2, fry desalination: dividing the fry into ponds, controlling the seawater salinity in the culture ponds, and carrying out desalination culture on the larimichthys crocea fry; and 3, transfer breeding, wherein after the fry desalination work in the step 2 is completed, the desalted larimichthys crocea fries are transferred to a salt and fresh water area to be bred; and the culture pond is a cement pond, a water salinity adjusting device is arranged above the cement pond, and a salinity monitoring device is arranged on one side of the cement pond. According to the method, the whole pond is fully covered with water, local salinity sudden change of water in the culture pond is greatly reduced, fry stress reaction caused by salinity change is reduced to the maximum extent, preparation of desalted fry of the small yellow croakers is achieved, and therefore the yield of small yellow croaker culture is increased.

The invention discloses an efficient screening method for parents required by preparation of a fast-growing larimichthys polyactis new strain, and belongs to the technical field of larimichthys polyactis parent screening. The screening method comprises the steps that a larimichthys polyactis family is constructed, PIT marking is carried out on larimichthys polyactis with the high survival rate, recovering and temporary culturing are carried out on larimichthys polyactis with PIT marking finished, body mass measuring and breeding performance evaluating are carried out, and parents required by preparation of the fast-growing new strain are recognized and screened out. According to the method, the accuracy of excellent parent selection can be remarkably improved, and a foundation is laid forfast obtaining the fast-growing larimichthys polyactis new strain. By the adoption of the method, relative genetic progress of 5.38% can be obtained for each generation. By comparing breeding value selection and phenotype selection effects, it can be found that the breeding value selection accuracy is improved by 94.35% relative to phenotype selection.

The present invention relates to an enzyme-type natural seafood powder and a preparation method thereof. The seafood powder uses larimichthys polyactis and short necked clams as main raw materials and is prepared by enzymolyzing and blending. The mass ratio of the larimichthys polyactis and short necked clams is 1 : (0.1-10) and more preferably is 1 : 1. The obtained seafood powder has both olfactory sense and mouthfeel, is long in maintaining time, tastes full and prominent, has a natural seafood flavor, and is mellow and delicious.

The invention relates to the technical field of bean food processing, and particularly relates to a process for fermenting stinky preserved soybeans. The process comprises the following steps: washing high-quality soybeans, drying in the air, roasting, soaking, and mixing with little yellow croaker to ferment for 5-7 days in a sealed condition; separating soybeans from little yellow croaker, steaming, inoculating one or more of aspergillus niger, rhizopus oryzae and glucoamylase in the steamed soybeans, seasoning the fermented soybeans, filling into cans, and pasteurizing to obtain a finished product. The stinky preserved soybeans prepared by the process have unique flavor and mellow taste, and can be used for promoting elimination of heavy metals in a body. According to the stringy fermented soybeans, the protein is increased by 30-35 percent, the total amino acid is increased by 22-26 percent, the large molecular weight protein degradation rate is more than 99.2 percent, and the small molecular protein is more than 50.1 percent of soluble protein. The stinky preserved soybeans can be stored for 8 months at normal temperature when chemical preservative is not added.

The invention discloses a little yellow croaker cultivating method in high female rate, and a fodder feeding device. An objective of the method is to solve problems that female rate of little yellow croakers obtained by an artificial breeding method is relatively low, fodder easily sinks to the bottom of a pool after feeding, and the fodder is wasted. The method comprises the following steps: a, cultivating fries, using soybean milk to perform nutrient enrichment on rotifer which is initial feed of little yellow croakers, 5 days after the little yellow croakers are hatched, bailing out the nutrient-enriched rotifers and feeding into a little yellow croaker culture pond, continuously feeding for 10 days; b, culturing in an early stage, after 15 day-old of the little yellow croakers, starting to feed yolk; c, culturing in a medium stage, after 30 day-old of the little yellow croakers, controlling water temperature of the culture pond between 20 and 22 DEG C, wherein in 30 day-old to 40 day-old, feeding soybean milk enriched fairy shrimps, after 40 day-old and before 90 day-old, feeding an artificial compound feed; d, culturing in a later stage, after the little yellow croaker are 90day old, water temperature of the culture pond recovering to water temperature of normal seawater, and continuing to feed the artificial compound feed.