The invention discloses a preparation method of oil
soybean meal protein and an application of oil
soybean meal protein in a
beef sausage. The preparation method of the oil
soybean meal protein comprises the following steps: carrying out supercritical oil extraction on
cyperus esculentus,
caking, crushing, screening,
degreasing and adopting three times of ultrasonic auxiliary alkali leaching processes, wherein the first alkaline leaching process adopts the ratio of materials to liquid of 1 to 17, the
ultrasound time of 26 minutes and the pH value of 9.6, and the second and the third alkaline leaching processes adopt the ratio of the materials to the liquid of 1 to 10, the pH value of 9.6, and the
ultrasound time of 26 minutes; rotating by 3000 r / minute, centrifuging for 15 minutes, combining an alkali leaching liquid obtained in three times, carrying out acid
precipitation for 1 hour under the condition that the pH value is 4.2, rotating by 3000 r / minute, centrifuging for 15 minutes, washing protein milk for 30 minutes by using 40-DEG C water with a weight of 5 times of the protein milk, washing for three times, and freezing and
drying to obtain the oil soybean
meal protein. The preparation method of the oil soybean
meal protein and the application of the oil soybean
meal protein in the
beef sausage have the benefits that the yield of the applied
beef sausage is improved by 21.59 percent, which is improved by 11.52 percent compared with the applied beef sausage added with
soybean protein; the
chewiness, the
hardness, elasticity and other sausage gel indicators of the beef sausage can be obviously improved, and the application is wide.