Construction method for inducing larimichthys polyactis liver cell apoptosis model through high-temperature stress

A technology of liver cells and small yellow croaker, applied in climate change adaptation, animal husbandry, etc., can solve problems such as difficulty in standardization, large differences in research results, and little mutual reference between research results.
CN112790154AActive Publication Date: 2021-05-14ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Publication Date
2021-05-14

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Abstract

The invention belongs to the technical field of oxidative stress models, and particularly relates to a construction method for inducing a larimichthys polyactis liver cell apoptosis model through high-temperature stress. The construction method specifically comprises the following steps: when the culture water temperature is about 20 DEG C, treating larimichthys polyactis according to the heating rate of 2 DEG C / h, raising the water temperature to 32 + / -0.5 DEG C, and treating the larimichthys polyactis at the temperature for 6-12 h, so that the larimichthys polyactis is subjected to oxidative stress reaction to induce apoptosis. According to the construction method, the basic water temperature, the heating rate, the high temperature needing to be reached and the processing time of the high temperature before high-temperature stress are determined, and evaluation indexes for model establishment are provided. The larimichthys polyactis liver cell apoptosis model established through high-temperature stress can be used for researching pathogenesis of the larimichthys polyactis liver oxidative injury, and has important theoretical and practical significance for revealing the larimichthys polyactis liver oxidative injury mechanism and researching and developing efficient liver protection drugs.
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Description

technical field

[0001] The invention belongs to the technical field of oxidative stress model construction, and in particular relates to a method for constructing a liver cell apoptosis model induced by high temperature stress. Background technique

[0002] Small yellow croaker, as one of the "four seafoods" in my country, has very important economic value. With the maturity of large-scale artificial breeding and breeding technology, the small yellow croaker breeding industry will develop rapidly. In the breeding process, the temperature of the water body is directly related to the survival rate and growth rate of the breeding objects. Studies have shown that the water temperature of 32°C will cause oxidative stress in small yellow croakers, and in severe cases, it will lead to a large number of deaths. Therefore, elucidating the molecular mechanism of small yellow croaker's response to high temperature stress and providing effective technical means to relieve high tempera...

Claims

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