Larimichthys polyactis microsatellite DNA molecular marker

A technology of microsatellite markers and DNA molecules, applied in the direction of recombinant DNA technology, DNA / RNA fragments, etc., can solve the problem of limited quantity

Inactive Publication Date: 2013-01-16
SUN YAT SEN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only 35 microsatellite molecular markers developed so far for small yellow croaker (Chen et al., Conservation genetics2009, 10:1469-1471; Li et al., Molecular Ecology Notes 2006, 6(1):188-190; Ma et al., Genetics and Molecular Research 2011, 10(3...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Larimichthys polyactis microsatellite DNA molecular marker
  • Larimichthys polyactis microsatellite DNA molecular marker
  • Larimichthys polyactis microsatellite DNA molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] 1. Construction of microsatellite molecular marker enrichment library of small yellow croaker:

[0093] 1.1 Genomic DNA extraction:

[0094] Genomic DNA was extracted by the phenol-chloroform method, and the specific steps were as follows: Cut 20 mg of caudal fin into a 1.5 ml centrifuge tube, add 570 μL of STE buffer (10 mmol / L Tris-Cl, pH=8.0; 100 mmol / L EDTA, pH=8.0; 100 mmol / L L NaCl) and cut up the tissue. Add 10μL 20mg / ml proteinase K and 20μL 20% SDS, digest at 55-56℃. Add 600 μL of phenol:chloroform:isoamyl alcohol (25:24:1) to extract twice, centrifuge at 13000r / min for 5min, and take the supernatant. Add an equal volume of -20°C pre-cooled absolute ethanol, mix well, centrifuge at 13000r / min for 5min, discard the supernatant, wash the precipitate twice with 70% ethanol, dry at room temperature, then add 50-100μL TE buffer (1mmol / L EDTA, 10mmol / L Tris-HCl, pH=8.0) to dissolve DNA. The DNA concentration was detected by an ultraviolet spectrophotometer, dilu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a larimichthys polyactis microsatellite DNA molecular marker. The invention adopts the technical scheme that an enhanced library of larimichthys polyactis microsatellites (GT) 17, (GGT) 9 and (CTAT) 8 is built, larimichthys polyactis polymorphic microsatellite primers are screened, and the genetic polymorphism detection is carried out on larimichthys polyactis microsatellite loca, so that 21 larimichthys polyactis microsatellite markers with rich polymorphism are developed. The larimichthys polyactis microsatellite DNA molecular marker is applicable to researches on the population genetic structure and the genetic variation level of a larimichthys polyactis population.

Description

technical field [0001] The invention relates to a molecular marker technology, in particular to a small yellow croaker microsatellite DNA molecular marker. Background technique [0002] The small yellow croaker (Larimichthys polyactis) belongs to Perciformes, Totoabaidae, and Yellow Croaker. It is a warm-temperate bottom fish widely distributed in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. It is an important marine fishery economic species in my country. It is one of the "four major fisheries" in China. In recent years, the high-intensity fishing pressure has caused the small yellow croaker resources to be overfished. In order to protect and manage the fishery resources of small yellow croaker, it is very necessary to study the population genetic structure and genetic variation level. Microsatellite molecular markers have become the most commonly used method for genetic analysis of marine fish populations due to their advantages of high polym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C12Q1/68
Inventor 蒙子宁王乐林浩然
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap