Construction method of larimichthys polyactis growth hormone bioreactor silkworm strain

A technology of bioreactor and growth hormone, which is applied in growth hormone, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of small yellow croaker resource destruction and unstable output, so as to promote the growth of small yellow croaker and improve the benefits of sericulture industry , the effect of alleviating the development dilemma

Active Publication Date: 2021-01-01
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

However, due to the impact of overfishing and changes in the marine environment, the resources of small yellow croakers have been severely damaged, and the output is extremely unstable.

Method used

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  • Construction method of larimichthys polyactis growth hormone bioreactor silkworm strain
  • Construction method of larimichthys polyactis growth hormone bioreactor silkworm strain
  • Construction method of larimichthys polyactis growth hormone bioreactor silkworm strain

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Embodiment

[0037] (1) Cloning of small yellow croaker growth hormone gene and transferrin gene: using the whole genome sequencing information of small yellow croaker and transcriptome information of small yellow croaker, the growth hormone gene (GH) and transferrin gene (TF) of small yellow croaker were respectively obtained by homologous inspection method ) sequence, design the fusion gene of small yellow croaker growth hormone and transferrin; and optimize the fusion gene sequence according to the codon preference of silkworm. Before the fusion gene, add the IE2 promoter sequence, Kozak sequence and the coding sequence of the eukaryotic phosphate transport-related peptide in sequence, add the 6×His tag coding sequence and the SV40 sequence after the fusion gene, and finally add the coding sequence of the exogenous gene expression cassette to the two sides of the foreign gene expression cassette. The kpnⅠ and HindIII cloning sites were set at the end, and the synthetic insert was cloned ...

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Abstract

The invention discloses a construction method of a larimichthys polyactis growth hormone bioreactor silkworm strain, and belongs to the technical field of silkworm transgenic genetic breeding. The method comprises the following steps of (1) synthesizing a larimichthys polyactis growth hormone and transferrin fusion gene; (2) obtaining piggBAC transgenic plasmids pLX-Red-PcGH-TF; (3) introducing the transgenic plasmids into silkworm fertilized eggs; (4) obtaining G1-generation silkworm eggs; (5) screening marker gene positive individuals; (6) verifying efficient expression of larimichthys polyactis growth hormone in silkworm; (7) identifying genetic stability; and (8) establishing the larimichthys polyactis growth hormone bioreactor silkworm strain. According to the invention, on one hand,the larimichthys polyactis growth hormone is produced with high efficiency and low cost, and can be further processed into a larimichthys polyactis functional additive to be applied to larimichthys polyactis culture; and on the other hand, the development dilemma faced by silkworm production is relieved, and the effect of improving silkworm industry benefits is achieved.

Description

technical field [0001] The invention belongs to the technical field of silkworm transgenic genetic breeding, and in particular relates to a method for constructing a silkworm strain in a small yellow croaker growth hormone bioreactor. Background technique [0002] Since the reform and opening up, under the guidance of the development policy of "focusing on breeding", the development of aquaculture industry has made great achievements. The rapid development of the aquaculture industry has made outstanding contributions to solving the "difficulty in eating fish" of urban and rural residents, ensuring the supply of high-quality animal protein, reducing the utilization intensity of aquatic biological resources in natural waters, promoting the prosperity of the fishery industry, and making fishermen's lives rich. However, compared with the development requirements of the new era, the aquaculture industry still faces some difficulties and problems. In order to promote the green a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/62C12N15/866C12N15/66A01K67/04
CPCC07K14/61C07K14/79C12N15/86C12N15/66C12N15/8509A01K67/04A01K67/0333C12N2800/22C12N2710/14043C07K2319/21A01K2207/15A01K2217/072A01K2227/70A01K2267/01
Inventor 翁宏飚牛宝龙沈卫锋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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