Construction method of larimichthys polyactis growth hormone bioreactor silkworm strain
A technology of bioreactor and growth hormone, which is applied in growth hormone, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of small yellow croaker resource destruction and unstable output, so as to promote the growth of small yellow croaker and improve the benefits of sericulture industry , the effect of alleviating the development dilemma
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[0037] (1) Cloning of small yellow croaker growth hormone gene and transferrin gene: using the whole genome sequencing information of small yellow croaker and transcriptome information of small yellow croaker, the growth hormone gene (GH) and transferrin gene (TF) of small yellow croaker were respectively obtained by homologous inspection method ) sequence, design the fusion gene of small yellow croaker growth hormone and transferrin; and optimize the fusion gene sequence according to the codon preference of silkworm. Before the fusion gene, add the IE2 promoter sequence, Kozak sequence and the coding sequence of the eukaryotic phosphate transport-related peptide in sequence, add the 6×His tag coding sequence and the SV40 sequence after the fusion gene, and finally add the coding sequence of the exogenous gene expression cassette to the two sides of the foreign gene expression cassette. The kpnⅠ and HindIII cloning sites were set at the end, and the synthetic insert was cloned ...
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