Culturing method for cultured leguminous roots having increased coumestrol content

A technology of coumestrol and culturing method, which is applied in the directions of horticultural methods, tissue culture, cell culture medium and the like, and can solve problems such as pathways and complex chemical structures for which a synthesis method of coumestrol has not yet been found.

Pending Publication Date: 2019-05-03
AMOREPACIFIC CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex chemical structure in which a large number of aromatic rings are fused, no route has been found to develop a simple method for the synthesis of coumestrol
Although several synthetic methods have been reported, each method suffers from various problems, making commercialization challenging

Method used

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  • Culturing method for cultured leguminous roots having increased coumestrol content
  • Culturing method for cultured leguminous roots having increased coumestrol content
  • Culturing method for cultured leguminous roots having increased coumestrol content

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1-1

[0069] [Preparation Example 1-1] Germination of bean seeds and in vitro plant induction

[0070] Use 2% sodium hypochlorite to sterilize each surface of Myth bean seeds and Bean bean seeds for 20 min and wash 3 times with sterile water. Then, the plants were induced in 1 / 2 MS medium (Murashige and Skoog medium) supplemented with 30 g / L sucrose at 25±1°C under light conditions for 2 to 3 weeks.

[0071] The procedure for inducing isolated plants from leguminous seeds is as figure 1 shown.

preparation example 1-2

[0072] [Preparation Example 1-2] Induction of Bean Cultured Roots

[0073] The cotyledons, hypocotyls and radicles from the induced plants were cut into about 1 cm, and treated with IBA (indole butyric acid) and NAA (naphthalene acetic acid) in 1MS medium supplemented with 30 g / L sucrose, kept at 22±1° C. for 2 to 3 weeks under dark conditions to induce cultured roots.

[0074] Depending on the type of beans, the induction site, and the type and amount of induction of the auxin, the induction state such as figure 2 and image 3 shown.

experiment example 1

[0075] [Experimental Example 1] Determination of dry matter productivity and coumestrol content of cultured roots depending on bean variety, induction site, and type and amount of auxin

[0076] The dry matter productivity of the dried bean culture roots of Preparation Example 1-2 was measured. The result is as Figure 4 shown. The growth state of the bean cultured root differs depending on the kind of bean and the induction site. In particular, after 4 weeks of cultivation, the net weight of cultured roots derived from the radicles of Myth bean increased by 12.5 times relative to the initial value. The final dry matter productivity was also excellent at 4.1 g / L.

[0077]Further, the dried bean culture roots of Preparation Example 1-2 were extracted in 80% (w / v) ethanol at room temperature for 24 hours. The obtained extract was filtered with filter paper, and dried by evaporating the solvent to obtain a powder. Then, the powder was diluted into a 1% solution to prepare th...

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Abstract

The present specification relates to a culturing method for cultured leguminous roots having an increased coumestrol content, the method being capable of mass-producing coumestrol, which is present ina very small amount in a leguminous plant, wherein the culturing method comprises the steps of: (a) germinating leguminous seeds in a culture medium to induce in vitro plants having cotyledons, hypocotyls, and radicles; (b) culturing, in a culture medium, at least one site of the cotyledons, hypocotyls, and radicles of the induced in vitro plant to induce site-specific cultured roots; and (c) multiplying the induced site-specific cultured roots in a culture medium, wherein the culture media contain nutrient components of NH4NO3, CaCl2.2H2O, MgSO4.7H2O, KH2PO4, and KNO3.

Description

technical field [0001] The invention relates to a method for cultivating legume cultured roots with increased coumestrol content, and the leguminous cultured roots with increased coumestrol content obtained by the method. Background technique [0002] So far, coumestrol has been considered the most powerful substance among phytoestrogens. Coumestrol is mainly found in the seeds, roots and leaves of legumes and Compositae plants, and is generally classified as a coumestan compound, which is an isoflavone. Coumestrol has attracted attention because it is secreted in high concentrations at injured sites when plants are damaged and has antifungal and antiviral activities to prevent infection through antioxidant, anti-inflammatory and antitoxin activities. The reason for this is that infections due to various bacteria, fungi and viruses induce the synthesis of various aroma compounds including coumestrol. This antibiotic activity of coumestrol is based on its phenolic structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H5/10A01H6/54C12N5/00C12N5/04
CPCA01H4/005A01H6/54A01H6/542A01H4/002A01H5/06C12N5/04C12N5/0025
Inventor 李恩姃姜永圭朴浚星朴昭映金英恩
Owner AMOREPACIFIC CORP
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