68 results about "Leguminoseae" patented technology

The invention provides an MsLEA3 gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the alfalfa MsLEA3 gene is subjected to overexpression or gene editing is carried out on the MsLEA3 gene in alfalfa through a CRISPR / Cas9 system, so that an overexpression transgenic line of the alfalfa MsLEA3 gene or a gene editing transgenic line aiming at the alfalfa MsLEA3 gene is obtained. The salt tolerance of an overexpressed or gene-edited medicago sativa transgenic line of the MsLEA3 gene is remarkably improved, the MsLEA3 gene can be used for cultivating medicago sativa varieties with high salt resistance, a new theoretical basis and candidate genes are provided for cultivating new varieties of high-resistance leguminous forage, and the application of medicago sativa in production is greatly promoted.

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.

The invention provides application of an MsMYBS3 gene or a biological material thereof in regulation and control of plant cold resistance, and belongs to the technical field of plant genetic engineering. The amino acid sequence coded by the MsMYBS3 gene disclosed by the invention is as shown in SEQ ID NO. 4. The invention provides an application of a medicago sativa MYB transcription factor gene MsMYBS3 in the aspect of regulating and controlling the cold resistance of medicago sativa. A gene editing technology is adopted to directionally change the development and the cold resistance of the medicago sativa. The cold resistance of an MsMYBS3 gene editing strain constructed by the invention is reduced, and the MsMYBS3 gene has the effect of regulating and controlling the cold resistance of alfalfa, so that a theoretical basis and a candidate gene are provided for cultivating new varieties of high-resistance leguminous forage, and the application of alfalfa in production is promoted.

The invention discloses a method for improving the quality of cultivated land through mixed sowing of perennial beans, grasses and forage grass. The method comprises the steps that deep ploughing and soil turning are conducted, land leveling is conducted, and organic fertilizer is applied to serve as base fertilizer; the method comprises the following steps: determining a mixing ratio of leguminous forage grass to gramineous forage grass of 3: 7-1: 1, and carrying out mixing pretreatment on the two forage grass seeds, namely preparing a nano titanium dioxide solution containing various nutrients and trace elements, screening stress-resistant domesticated rhizobium, disinfecting, soaking, coating and the like; reasonably topdressing in the forage grass growth period; when the pasture grows to a specific stage, the pasture is cut for 2-3 times every year; after 3-5 years of continuous utilization, crop rotation is carried out, and deep ploughing and fertilization are carried out again. According to the seed mixing pretreatment mode, the operation process is simplified, the cost is reduced, balanced nutrition supply is guaranteed, synchronous germination of seeds and coordinated growth of seedlings are promoted, and the fertilizer utilization rate is increased. The screened adversity-resistant domesticated rhizobium enhances the stress resistance of mixed sowing grassland, improves the yield and quality of alfalfa, and further improves the overall quality of mixed sowing pasture.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The utility model discloses a water and nitrogen absorption root system culture observation device for a leguminous green manure intercropping field. The water and nitrogen absorption root system culture observation device comprises an outer frame, an observation tank, a supporting frame and a climbing rod. The outer frame is of a cuboid structure, a plurality of containing grooves are formed in the outer frame and used for containing observation tanks, and labels are arranged on the front portions of the containing grooves. The observation tank is a transparent cylindrical tank with an open top and a closed bottom, and the outer wall of the observation tank is attached to the inner wall of the containing groove. The supporting frame is located in the observation tank and comprises a vertically-arranged supporting rod and a fixing ring. The climbing rods are rotatably arranged in the supporting frame, and threaded grooves with different curvatures are formed in the surfaces of rod bodies. Root growth is directly observed through the transparent observation tank, the device does not need to be opened, the natural growth environment of the root is prevented from being disturbed, and observation accuracy is improved. The threaded groove of the climbing rod guides the root system to grow orderly, so that the root system is uniformly distributed, the root systems at different positions are conveniently and comprehensively observed, and the growth condition of the root system is accurately evaluated.

The present application belongs to the field of ecology and environmental monitoring technology, and relates to a kind of detection and analysis method for decomposition rate of leguminous sand-fixing shrub litter in field.The present application screens sample area by system grid method, collects and lays out litter bag, synchronously determines initial and current nitrogen content of soil, calculates net increment of soil nitrogen, determines horizontal diffusion boundary of nitrogen, and then obtains soil absorbed nitrogen amount;obtains equivalent dry matter loss amount based on nitrogen balance calibration, and finally determines litter decomposition rate by exponential decay fitting.The method solves the decomposition rate estimation deviation problem caused by nitrogen migration and soil absorption in traditional detection, can more accurately reflect the actual decomposition process in field, improves the reliability of monitoring data, and provides reliable technical support for nutrient cycling research and ecological restoration effect evaluation of desert ecosystem.

The application provides a kind of MsLEA3 Gene and its application belong to the technical field of plant genetic engineering.The application obtains the overexpression transgenic strain of alfalfa Medicago sativa L. MsLEA3 Gene or carries out gene editing to the gene of Medicago sativa L. MsLEA3 Through the overexpression of Medicago sativa L. MsLEA3 Gene or the gene editing transgenic strain of Medicago sativa L. MsLEA3 Gene.The overexpression or gene editing transgenic strain of Medicago sativa L. MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLE Gene significantly improves the salt tolerance of the transgenic strain, can be used for cultivating Medicago sativa L.varieties with high salt resistance, provides a new theoretical basis and candidate gene for cultivating new varieties of legume forage grass with high resistance, and greatly promotes the application of Medicago sativa L.in production.

The application discloses a kind of planting management methods for improving the yield of machine harvesting and rolling cane field ratoon cane, and relates to the technical field of sugarcane planting.The planting management method comprises the following steps: interplanting leguminous grass, applying microbial inoculant, timely ploughing and interplanting fat field radish.The planting management method can improve the physical and chemical structure of soil.Under the condition of mechanical high pressure, it can increase the ratoon cane sprouting rate, increase the number of effective stems and increase the plant height, thereby achieving the purpose of high yield and stable yield of ratoon cane for many years in succession.Meanwhile, the method is simple to operate, the effect is obvious, and is suitable for promotion.

This invention discloses a salt-tolerant Cladosporium 550W1 strain and its application in promoting nodulation and nitrogen fixation in leguminous plants, belonging to the field of microbial technology. The salt-tolerant Cladosporium (… Cladosporium halotolerans Cladosporium 550W1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 41943, provides a salt-tolerant Cladosporium 550W1 that can significantly promote nodulation and nitrogen fixation in legumes, increase plant biomass and nitrogen accumulation, and reduce dependence on chemical nitrogen fertilizers. It has broad application prospects and environmental and ecological benefits.

The present application relates to the field of agricultural soil improvement and orchard fertilization technology, and discloses a kind of southern red soil area orchard annual green manure covering and carbon-increasing quality improving method, including that mature livestock and poultry manure is mixed with industrial grade calcium-magnesium phosphate fertilizer and applied to surface soil before green manure sowing;Sowing Indian cowpea in spring, mixed sowing Chinese milk vetch and field radish in autumn;Green manure of leguminous plants is mowed in situ when it is in full bloom to initial pod stage to make it flat;Uniformly spray directional wood-decay fungi complex liquid to the surface of green manure;Use pyroligneous acid to wet crushed dry crop straw, cover micro-acid straw on the surface of green manure;Mechanically subsoil in the following autumn, and inject PAM composite biomass charcoal suspension into the bottom of subsoiling groove simultaneously.The present application neutralizes and intercepts ammonia gas released by green manure to block nitrogen volatilization by using micro-acid straw, accelerates the degradation of macromolecular lignocellulose by using wood-decay fungi, and constructs deep stable carbon sink by combining with deep injection of porous carbon suspension, thereby effectively improving the overall carbon pool capacity of red soil profile.

This invention relates to a novel method for obtaining aqueous dispersions of modified legume proteins with improved colloidal stability using protein deamidases.

The invention belongs to the technical field of ecology and environmental monitoring, and relates to a field decomposition rate detection and analysis method for leguminous sand-fixing shrub litters. The method comprises the following steps: screening a sample area through a system grid method, collecting and arranging litter bags, synchronously measuring initial and current nitrogen contents of soil, calculating a soil nitrogen net increment, determining a nitrogen transverse diffusion boundary, and further obtaining the nitrogen absorption amount of the soil; the equivalent dry matter loss amount is obtained based on nitrogen element balance calibration, and finally the litter decomposition rate is determined through exponential decay fitting. According to the method, the problem of decomposition rate estimation deviation caused by nitrogen migration and soil absorption in traditional detection is solved, the actual field decomposition process can be reflected more accurately, the reliability of monitoring data is improved, and reliable technical support is provided for desert ecosystem nutrient cycle research and ecological restoration effect evaluation.

The present application belongs to the field of microbial technology. More particularly, it relates to a salt-tolerant Bradyrhizobium sp. strain RY15 and its application. The Bradyrhizobium sp. strain RY15 provided by the present application has excellent salt tolerance and can significantly promote the growth and development and nodule fixation ability of Desmodium velutinum. The pot experiment results prove that the application of the RY15 strain can significantly increase the fresh weight of the aboveground part, the fresh weight of the underground part and the number of nodules of Desmodium velutinum; in the saline soil field, the yield of the pasture is increased by 68.89% after the inoculation of the RY15 inoculant. The RY15 strain of the present application can be made into a liquid or solid microbial inoculant, which is suitable for seed dressing, root soaking or soil application, and is simple to operate, low in cost and easy to popularize. The RY15 strain of the present application is a natural symbiotic bacterium, is safe and non-toxic, can improve the nitrogen cycle of soil, expand the planting of high-quality leguminous forage in saline-alkali soil, and has good application prospect and application value.

The present application provides a pregelatinized hydroxypropylated pea starch that has improved water-resistant film forming property compared to previously known film forming pea starches. The starch may be characterized by a RVA viscosity profile. It may be obtained by the pregelatinization of a hydroxypropylated pea starch, notably on a drum dryer. The improved water-resistance was illustrated on films immersed into water and was also characterized by the determination of the hydrophobicity value of the starch using force tensiometry, goniometry and the Young-Laplace and Fowkes model.

The present invention relates to a highly soluble legume starch having a content of less than 10%, more preferably less than 6% by weight of oligosaccharides with a degree of polymerization (DP) of 1 and 2; a content of more than 50%, more preferably more than 70% by weight of oligosaccharides with a DP of 3 to 20; a water solubility of more than 90%, more preferably more than 95% by weight; a viscosity of less than 500 cP, more preferably less than 100 cP, and characterized by an α1,4 / α1,6 ratio of between 25% and 35%, preferably between 28% and 32% determined by 13 C NMR.

This utility model discloses a handheld seeder for small-particle legume forage, including a bracket, a connecting plate, a support column, a limiting cylinder, and a limiting rod. The bracket is fixedly connected to both sides of the connecting plate, and the support column is fixedly connected to both sides of the top right side of the connecting plate. This utility model, by incorporating a handle shock-absorbing mechanism, uses the cooperation of movable shaft one, inclined rod, sliding sleeve, movable shaft two, and the limiting rod to buffer the vibration generated during seeder movement. Combined with the rubber pad covering the surface of the push rod, it effectively reduces the transmission of vibration to the operator's hand, alleviating hand fatigue and allowing the operator to more easily hold and push the seeder, improving operational comfort and stability, thereby ensuring the continuity and efficiency of the sowing operation. It also has the advantage of reducing hand fatigue during use.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust) of the plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Provided is use of MfERF026 regulation in growth, development, and stress tolerance of Medicago sativa, which belongs to the technical field of plant genetic engineering. Provided is use of a regulatory agent for MfERF026, which is an ethylene responsive factor (ERF) in Medicago falcata, in promoting growth and development and improving drought tolerance and cold tolerance of M. sativa. The inhibited expression of MfERF026, the ERF in M. falcata may enhance the drought tolerance and cold tolerance of the M. sativa. Moreover, the inhibited expression of MfERF026, the ERF in M. falcata, may regulate the growth and development of M. sativa, thereby accelerating the growth rate of M. sativa to increase the number of leaves. The inhibited expression of MfERF026 provides a novel theoretical basis and a candidate gene for cultivating novel leguminous forage varieties with high resistance, yield, and protein content.

The invention provides an intercropping method for realizing salinity regulation and control and nitrogen synergy through sophora alopecuroide-filmless cotton space configuration, and belongs to the technical field of saline-alkali soil ecological farming. Wide and narrow rows are arranged, planting is carried out alternately, sophora alopecuroide is sown in the wide row to serve as a sophora alopecuroide belt, and cotton is sown in the narrow row to serve as a cotton belt; respectively performing drip irrigation in a seedling stage and a squaring stage; and cutting and returning to the field. By adjusting the spatial configuration of the leguminous shrub sophora alopecuroides and the film-free cotton, the mulching film pollution is eradicated; sophora alopecuroides root growth can improve the soil porosity and promote salt leaching, the overground part can cover and block evaporation salt return, and the salt surface accumulation problem of the film-free cotton field is solved; sophora alopecuroides nitrogen fixation substitution can reduce nitrogen fertilizer consumption. Sophora alopecuroides green manure returning to the field can improve soil organic matters and realize synchronous improvement of carbon and nitrogen. According to the method, plant ecological niche regulation is taken as a core, the cost is low, four-dimensional collaboration of salt inhibition, nitrogen increase, pollution reduction and synergism of the saline-alkali soil cotton field can be realized, and a standardized solution is provided for agricultural green transformation in arid regions.

The present application relates to the technical field of ecological restoration, and specifically discloses a method for repairing degenerated alpine meadow bare patches by using soil-plant feedback. The method comprises the following steps: separately planting candidate species in degenerated soil and non-degenerated soil, and screening suitable complementary species for degenerated soil; grouping the screened species according to functional groups, and planting the species in degenerated soil to determine an optimized species grouping scheme for different restoration targets. Through a two-stage screening system, three efficient groupings are obtained: a pure grass combination for rapid coverage and surface stabilization, a single legume + grass combination for stress resistance and balanced restoration effect, and a high-diversity double legume combination for long-term improvement and fertilization, thereby providing a scientific and accurate grouping decision-making tool for repairing degenerated alpine meadow bare patches.

The invention relates to a feed capable of preventing abortion of pregnant horses and a preparation method and application thereof, the feed is composed of corn straw, leguminous forage and non-leguminous forage, the feed contains 0-10% by mass of leguminous forage, and the total amount of the corn straw and the leguminous forage contained in the feed is less than or equal to 50%. The composition, proportion and nutritional ingredients of leguminous forage, non-leguminous forage and corn straw in the pregnant horse feed are scientifically adjusted, the content of conjugated estrogen in pregnant horse urine is remarkably increased, the content of unknown impurities B and C in the pregnant horse urine is remarkably reduced, and the separated and purified conjugated estrogen raw material medicine has the advantages of being high in extraction rate, good in stability and the like. The adverse reactions such as diarrhea, abdominal distension and incapability of lying on the ground of the pregnant horse and the abortion rate of the pregnant horse are obviously reduced.

The invention discloses a precise green manure management method for improving orchard soil quality, which comprises the following steps: S1, soil diagnosis: analyzing physicochemical properties of orchard soil, and determining main limiting factors of soil quality; s2, green manure preparation: based on the main limiting factors, selecting at least two types of green manure with complementary functions to construct a green manure combination, the green manure combination comprising a leguminous green manure and a non-leguminous green manure; s3, planting and growth management: sowing the green manure combination in a mixed sowing or intercropping mode; s4, turning, pressing and returning to the field: cradling and turning into the soil in the later growth period of the green manure. Through directional regulation and control of bean-straw green manure combination, available nutrients, organic matters and microbial biomass of soil can be effectively improved, the problem of soil degradation caused by long-term cleaning and ploughing is remarkably solved, and an efficient and accurate technical scheme is provided for sustainable management of orchard soil.

This invention discloses overexpression cysK The application of genes in increasing the soluble protein content of leguminous forage grasses belongs to the field of biotechnology. cysK The CDS sequence of the gene is shown in SEQ ID NO. 2. A plant overexpression vector containing the nucleotide sequence shown in SEQ ID NO. 2 was constructed, and the plant overexpression vector was introduced into leguminous forage plant cells to obtain overexpressed genes. cysK Transgenic leguminous forage lines with overexpressed genes in alfalfa. cysK The gene significantly increases the soluble protein content in its leaves by approximately 28%. This invention is the first to utilize Escherichia coli derived from prokaryotes. cysK The successful application of genes to the quality improvement of leguminous forage grasses has overcome the technical barrier of distant species kinship, providing new genetic resources and efficient technical approaches for breeding new varieties of leguminous forage grasses with high protein and high nutritional value, which is of great significance for promoting the sustainable development of the grass animal husbandry industry.

The present application relates to the technical field of agricultural planting, in particular to a coconut date coffee forage grass compound interplanting cultivation method, during 5-8 years before canopy formation after coconut date is planted in single planting with a plant row spacing of 6-10 m, a three-layer stereoscopic ecological niche complementary system is constructed in the row: the upper layer coconut date provides 30%-50% shading; the middle layer is a small-grain coffee planted in a strip outside the trunk at a distance of greater than or equal to 2 m; the lower layer is a strip-sowing creeping tropical leguminous forage grass, which reduces the ground surface temperature from greater than or equal to 55 DEG C to less than or equal to 35 DEG C, and is combined with three-track layered irrigation and mowing residual stubble ground surface coverage management, so that the irrigation water use efficiency is increased from less than 30% to greater than or equal to 65%, and the soil organic matter is accumulated from less than 0.5% to greater than or equal to 1.2%.

The application relates to the technical field of seed seedling raising, and provides a seedling raising method suitable for seeds of the genus Ormosia and application thereof, the seedling raising method comprises the following steps: (1) in early March, the seeds of the genus Ormosia are put into hot water with a temperature of 60-80 DEG C, the water is naturally cooled, the seeds are taken out after being absorbed, and the seeds are sprayed with water to keep moist; (2) step (1) is repeated once a day, and the process is continued for 5-7 days, so that the seeds are obtained after preliminary treatment; (3) the seeds after preliminary treatment are soaked in a suspension seed coating agent for 10-18 hours, so that the seeds after soaking are obtained; (4) the seeds after soaking are inoculated into a substrate to accelerate germination for 30-50 days. The seedling raising method can improve the germination rate and germination uniformity of the seeds of the genus Ormosia, and simultaneously improve the quality of seedlings, increase the height and the number of lateral roots of the seedlings. The seedling raising method is low in cost, wide in applicability, easy to popularize, and can provide reliable technical support for seedling raising of the seeds of the genus Ormosia of the legume family.

The invention relates to the field of agricultural planting, and discloses a mixed sowing cultivation method for high-stem gramineous forage grass and short-stem leguminous forage grass, which comprises the following steps: step 1, species selection and proportioning, and step 2, zoned and layered sowing. In the invention, the seed mass ratio of the high-stem gramineous forage grass to the short-stem leguminous forage grass is set to be 3: 1-5: 1, and the seeds are sown in different areas; regulation and control of a mixed sowing grassland community structure and a production target are achieved, producers can flexibly select according to requirements, in addition, by sowing high-stalk gramineous forage grass and short-stalk leguminous forage grass in a partitioned and layered mode, interspecific competition of the high-stalk forage grass and the short-stalk forage grass is effectively relieved, the nutrient requirements of different forage grass are met in combination with differentiated water and fertilizer treatment, and the yield of the mixed sowing grassland is improved. The community structure is further optimized, the problems that in the prior art, yield and quality are difficult to consider, soil fertility declines, management is extensive and the like are solved, and an advanced technical scheme with highly unified economic benefits, social benefits and ecological benefits is provided for modern grass and animal husbandry.

The application relates to the field of agricultural technology and discloses an acidified tobacco field soil ecological restoration method, which comprises the following steps: firing tobacco residues and rice husks into wood ash, and applying the wood ash and a solid microbial agent to acidified tobacco field soil; planting leguminous plants and onions in the acidified soil; during the growth stage of the leguminous plants, picking the fruiting parts of the leguminous plants to obtain leguminous plant fruits and leguminous plant residues; turning the onions and the leguminous plant residues into green manure and pressing them into the acidified soil; processing the leguminous plant fruits into organic fertilizer, applying the organic fertilizer to the acidified soil, and completing the restoration of the acidification of the soil; the soil acidification restoration method provided by the application combines different measures to form a multi-level restoration strategy, improves the physical, chemical and biological properties of the soil from different angles, and effectively completes the restoration of the soil acidification.

This invention relates to a cultivation method for intercropping night-blooming jasmine with snow peas. The method includes steps such as land preparation and basal fertilizer application, transplanting of night-blooming jasmine, sowing of snow peas, harvesting of night-blooming jasmine, and harvesting of snow peas. This intercropping technique fully utilizes the complementary characteristics of perennial vines and leguminous climbing vegetables. Through integrated technology across the entire process, it achieves synergistic utilization of trellis resources and improved land utilization, while also offering advantages such as cost reduction, efficiency improvement, green ecology, and strong practicality. Compared to monoculture of night-blooming jasmine, this technique increases yield by 35.2%, reduces fertilizer and pesticide use by more than 20%, and also enriches the soil, providing a practical technical solution for intercropping specialty vegetables.