96 results about "Leguminoseae" patented technology

The utility model relates to the field of agricultural mechanical equipment, in particular to a handheld seeder for small-particle leguminous forage grass. The utility model provides the handheld seeder for the small-particle leguminous forage grass, which can be used for conveniently adjusting and controlling the seeding spacing of forage grass seeds, improving the seeding efficiency and ensuring the seeding uniformity and consistency. A handheld seeder for small-particle leguminous forage grass comprises a support, a soil covering pressing wheel, a seeding rod and the like, the left side of the lower portion of the support is rotationally connected with the soil covering pressing wheel, and the lower portion of the support is rotationally and detachably connected with the seeding rod. When the support moves, the sowing plough turns over a planting field, meanwhile, the soil covering pressing wheel rolls, the sowing rod rotates, seeds are sown into the ploughed planting field, and the sowing distance can be controlled by changing the size of the belt roller. The effects of conveniently adjusting and controlling the sowing spacing of the forage grass seeds, improving the sowing efficiency and ensuring the sowing uniformity and consistency are achieved.

The invention provides a regional soil quality improvement method based on a crop rotation mode, and relates to the technical field of soil quality optimization, and the method comprises the following steps: S1, establishment of the crop rotation mode: establishing a crop rotation system with leguminous forage grass and gramineous crops as cores in a target area, the leguminous forage grass being medicago sativa, the gramineous crops being corn, and the gramineous crops being corn; a carousel or intercropping mode is adopted, the carousel mode is a period of 2-3 years, alfalfa is planted in the first year to the second year, and corn is planted in the third year; according to the intercropping mode, the corn and the alfalfa are planted according to the row ratio of 2: 1-3: 1, the row spacing of the corn ranges from 60 cm to 70 cm, and the row spacing of the alfalfa ranges from 30 cm to 35 cm. According to the method, alternate sowing and intercropping of the medicago sativa and the corn are combined, symbiotic nitrogen fixation of rhizobium of the medicago sativa and absorption of deep potassium by deep root systems are utilized, surface nutrients are utilized in cooperation with shallow root systems of the corn, a layered nutrient utilization mechanism of shallow nitrogen fixation and deep potassium release is formed, and compared with a traditional single cropping mode, the soil improvement quality is improved; the problems of regional surface soil nitrogen loss and low deep nutrient utilization rate are solved.

The invention provides an MsLEA3 gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the alfalfa MsLEA3 gene is subjected to overexpression or gene editing is carried out on the MsLEA3 gene in alfalfa through a CRISPR / Cas9 system, so that an overexpression transgenic line of the alfalfa MsLEA3 gene or a gene editing transgenic line aiming at the alfalfa MsLEA3 gene is obtained. The salt tolerance of an overexpressed or gene-edited medicago sativa transgenic line of the MsLEA3 gene is remarkably improved, the MsLEA3 gene can be used for cultivating medicago sativa varieties with high salt resistance, a new theoretical basis and candidate genes are provided for cultivating new varieties of high-resistance leguminous forage, and the application of medicago sativa in production is greatly promoted.

The invention discloses a planting method for overcoming continuous cropping obstacles of silage corn, which solves the problems of soil degradation and diseases and insect pests caused by continuous cropping by integrating soil physical remediation, chemical remediation, biological remediation, microbial community regulation and ecological intercropping technologies. The core technology content comprises the steps that deep ploughing is combined with organic matter and a biochar modifier to repair the soil structure, a compound microbial agent is sprayed to inhibit pathogenic bacteria, leguminous green manure and commercial crop rape are used for improving nitrogen circulation and reducing the autotoxicity effect every two years, and a precise fertilization management technology is matched. After the scheme is implemented, the soil health condition is remarkably improved, the silage corn yield is remarkably increased, and good ecological and economic benefits are achieved.

The invention discloses a straw returning method based on corn and covered crop cultivation, which comprises the following steps: grading and crushing corn straws according to soil types, carrying out zoning treatment, combining a leguminous-gramineae-cruciferae ternary mixed sowing covered crop system, and matching a decomposition microbial inoculum and controlled release nitrogen fertilizer synergistic regulation technology. The efficient decomposition of the straws and the improvement of the soil quality are realized. The method can increase soil organic matters by 0.25%, increase corn yield by 12.7% and reduce nitrogen fertilizer loss by more than 30%, and is suitable for sustainable production in dry farming areas.

The invention provides a cultivated land conservation method for wheat straw ridge covering and no-tillage ditch sowing of green manure, and particularly belongs to the technical field of cultivated land conservation. The cultivated land conservation method comprises the following steps that wheat is harvested, wheat straw is totally returned to the field, and stubbles of 20-25 cm are reserved; after wheat is harvested, leguminous green manure is sown in a no-tillage mode; no chemical fertilizer is applied in the green manure growth process, and irrigation is conducted in furrows in the seedling stage or the squaring stage; the green manure naturally dies and covers the earth surface, and no-tillage wheat sowing is carried out in the wheat sowing season; and repeating the steps to carry out the next round of planting. The cultivated land conservation method is remarkable in water-saving effect, has nitrogen-saving and carbon-sequestration effects, can increase the biomass of green manure and increase the yield of wheat, has small influence on the environment, and can realize cultivated land conservation.

The invention provides a method for improving the productivity of degraded grazing grassland, and belongs to the technical field of grassland restoration. The method comprises the following steps that water and fertilizer automatic management devices are installed on the grazing grassland needing to be repaired every 2 m; the gramineous forage grass is sown in ditches, then the two kinds of leguminous forage grass are evenly mixed, and the mixed forage grass and the gramineous forage grass are sown in a cross mode; a wireless sensor system is arranged in the automatic water and fertilizer management device, the soil moisture content, the soil nutrient content and the vegetation growth condition are monitored through the wireless sensor system, and precise irrigation and precise fertilization are conducted according to monitoring data. By means of the measures of intelligent fertilization irrigation and no-tillage reseeding, the productivity of the degraded grassland is improved. The fertilization time, the use amount and the field moisture capacity of the grass on the grassland are strictly controlled, so that the absorption of the grass on the grassland to nutritional ingredients can be promoted, and the productivity of the grassland and the yield of the grass are further improved.

The invention provides a density-increasing and nitrogen-reducing efficient planting method for intercropping corn with leguminous green manure, and the method specifically comprises the following steps: 1, mixing corn seeds with a seed accelerating agent, and soaking; mixing and stirring the leguminous green manure plant seeds and the coating agent; step 2, optimizing wide and narrow row spacing, sowing corn seeds, and sowing leguminous green manure plant seeds in a peak shifting manner; 3, base fertilizer is applied along with ploughing, and additional fertilizer, nitrogen fertilizer and leaf fertilizer are applied in the corn growing period; 4, water quantity management in the corn growing period; 5, leguminous green manure plants are cut, turned and pressed, and a decomposition agent is applied; the problems of low planting density, high nitrogen fertilizer dependence, insufficient green manure utilization rate and the like in the existing corn intercropping leguminous green manure technology are solved by optimizing wide and narrow rows, enhancing the nitrogen fixation capability of the green manure, efficiently turning and pressing the green manure and the like.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.

The invention provides application of an MsMYBS3 gene or a biological material thereof in regulation and control of plant cold resistance, and belongs to the technical field of plant genetic engineering. The amino acid sequence coded by the MsMYBS3 gene disclosed by the invention is as shown in SEQ ID NO. 4. The invention provides an application of a medicago sativa MYB transcription factor gene MsMYBS3 in the aspect of regulating and controlling the cold resistance of medicago sativa. A gene editing technology is adopted to directionally change the development and the cold resistance of the medicago sativa. The cold resistance of an MsMYBS3 gene editing strain constructed by the invention is reduced, and the MsMYBS3 gene has the effect of regulating and controlling the cold resistance of alfalfa, so that a theoretical basis and a candidate gene are provided for cultivating new varieties of high-resistance leguminous forage, and the application of alfalfa in production is promoted.

The invention discloses a method for improving the quality of cultivated land through mixed sowing of perennial beans, grasses and forage grass. The method comprises the steps that deep ploughing and soil turning are conducted, land leveling is conducted, and organic fertilizer is applied to serve as base fertilizer; the method comprises the following steps: determining a mixing ratio of leguminous forage grass to gramineous forage grass of 3: 7-1: 1, and carrying out mixing pretreatment on the two forage grass seeds, namely preparing a nano titanium dioxide solution containing various nutrients and trace elements, screening stress-resistant domesticated rhizobium, disinfecting, soaking, coating and the like; reasonably topdressing in the forage grass growth period; when the pasture grows to a specific stage, the pasture is cut for 2-3 times every year; after 3-5 years of continuous utilization, crop rotation is carried out, and deep ploughing and fertilization are carried out again. According to the seed mixing pretreatment mode, the operation process is simplified, the cost is reduced, balanced nutrition supply is guaranteed, synchronous germination of seeds and coordinated growth of seedlings are promoted, and the fertilizer utilization rate is increased. The screened adversity-resistant domesticated rhizobium enhances the stress resistance of mixed sowing grassland, improves the yield and quality of alfalfa, and further improves the overall quality of mixed sowing pasture.

The invention relates to the technical field of green prevention and control of crops, in particular to an ecological synergistic prevention and control method for diseases and insect pests suitable for strawberry planting in plateau regions, which attracts natural enemy insects by intercropping umbelliferae, compositae and leguminous functional plants, and naturally controls the population number of honeydew type pests such as trialeurodes vaporariorum, aphids and scale insects. The soil fertility is maintained through green manure rotation, the strawberry sooty mould occurrence rate is effectively reduced, the strawberry fruit quality and yield are improved, and the method has the advantages of being green, safe, low in investment and high in benefit.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The utility model discloses a water and nitrogen absorption root system culture observation device for a leguminous green manure intercropping field. The water and nitrogen absorption root system culture observation device comprises an outer frame, an observation tank, a supporting frame and a climbing rod. The outer frame is of a cuboid structure, a plurality of containing grooves are formed in the outer frame and used for containing observation tanks, and labels are arranged on the front portions of the containing grooves. The observation tank is a transparent cylindrical tank with an open top and a closed bottom, and the outer wall of the observation tank is attached to the inner wall of the containing groove. The supporting frame is located in the observation tank and comprises a vertically-arranged supporting rod and a fixing ring. The climbing rods are rotatably arranged in the supporting frame, and threaded grooves with different curvatures are formed in the surfaces of rod bodies. Root growth is directly observed through the transparent observation tank, the device does not need to be opened, the natural growth environment of the root is prevented from being disturbed, and observation accuracy is improved. The threaded groove of the climbing rod guides the root system to grow orderly, so that the root system is uniformly distributed, the root systems at different positions are conveniently and comprehensively observed, and the growth condition of the root system is accurately evaluated.

The present application belongs to the field of ecology and environmental monitoring technology, and relates to a kind of detection and analysis method for decomposition rate of leguminous sand-fixing shrub litter in field.The present application screens sample area by system grid method, collects and lays out litter bag, synchronously determines initial and current nitrogen content of soil, calculates net increment of soil nitrogen, determines horizontal diffusion boundary of nitrogen, and then obtains soil absorbed nitrogen amount;obtains equivalent dry matter loss amount based on nitrogen balance calibration, and finally determines litter decomposition rate by exponential decay fitting.The method solves the decomposition rate estimation deviation problem caused by nitrogen migration and soil absorption in traditional detection, can more accurately reflect the actual decomposition process in field, improves the reliability of monitoring data, and provides reliable technical support for nutrient cycling research and ecological restoration effect evaluation of desert ecosystem.

The application provides a kind of MsLEA3 Gene and its application belong to the technical field of plant genetic engineering.The application obtains the overexpression transgenic strain of alfalfa Medicago sativa L. MsLEA3 Gene or carries out gene editing to the gene of Medicago sativa L. MsLEA3 Through the overexpression of Medicago sativa L. MsLEA3 Gene or the gene editing transgenic strain of Medicago sativa L. MsLEA3 Gene.The overexpression or gene editing transgenic strain of Medicago sativa L. MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLE Gene significantly improves the salt tolerance of the transgenic strain, can be used for cultivating Medicago sativa L.varieties with high salt resistance, provides a new theoretical basis and candidate gene for cultivating new varieties of legume forage grass with high resistance, and greatly promotes the application of Medicago sativa L.in production.

The application discloses a kind of planting management methods for improving the yield of machine harvesting and rolling cane field ratoon cane, and relates to the technical field of sugarcane planting.The planting management method comprises the following steps: interplanting leguminous grass, applying microbial inoculant, timely ploughing and interplanting fat field radish.The planting management method can improve the physical and chemical structure of soil.Under the condition of mechanical high pressure, it can increase the ratoon cane sprouting rate, increase the number of effective stems and increase the plant height, thereby achieving the purpose of high yield and stable yield of ratoon cane for many years in succession.Meanwhile, the method is simple to operate, the effect is obvious, and is suitable for promotion.

This invention discloses a salt-tolerant Cladosporium 550W1 strain and its application in promoting nodulation and nitrogen fixation in leguminous plants, belonging to the field of microbial technology. The salt-tolerant Cladosporium (… Cladosporium halotolerans Cladosporium 550W1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 41943, provides a salt-tolerant Cladosporium 550W1 that can significantly promote nodulation and nitrogen fixation in legumes, increase plant biomass and nitrogen accumulation, and reduce dependence on chemical nitrogen fertilizers. It has broad application prospects and environmental and ecological benefits.

The present application relates to the field of agricultural soil improvement and orchard fertilization technology, and discloses a kind of southern red soil area orchard annual green manure covering and carbon-increasing quality improving method, including that mature livestock and poultry manure is mixed with industrial grade calcium-magnesium phosphate fertilizer and applied to surface soil before green manure sowing;Sowing Indian cowpea in spring, mixed sowing Chinese milk vetch and field radish in autumn;Green manure of leguminous plants is mowed in situ when it is in full bloom to initial pod stage to make it flat;Uniformly spray directional wood-decay fungi complex liquid to the surface of green manure;Use pyroligneous acid to wet crushed dry crop straw, cover micro-acid straw on the surface of green manure;Mechanically subsoil in the following autumn, and inject PAM composite biomass charcoal suspension into the bottom of subsoiling groove simultaneously.The present application neutralizes and intercepts ammonia gas released by green manure to block nitrogen volatilization by using micro-acid straw, accelerates the degradation of macromolecular lignocellulose by using wood-decay fungi, and constructs deep stable carbon sink by combining with deep injection of porous carbon suspension, thereby effectively improving the overall carbon pool capacity of red soil profile.

This invention relates to a novel method for obtaining aqueous dispersions of modified legume proteins with improved colloidal stability using protein deamidases.

The invention belongs to the technical field of ecology and environmental monitoring, and relates to a field decomposition rate detection and analysis method for leguminous sand-fixing shrub litters. The method comprises the following steps: screening a sample area through a system grid method, collecting and arranging litter bags, synchronously measuring initial and current nitrogen contents of soil, calculating a soil nitrogen net increment, determining a nitrogen transverse diffusion boundary, and further obtaining the nitrogen absorption amount of the soil; the equivalent dry matter loss amount is obtained based on nitrogen element balance calibration, and finally the litter decomposition rate is determined through exponential decay fitting. According to the method, the problem of decomposition rate estimation deviation caused by nitrogen migration and soil absorption in traditional detection is solved, the actual field decomposition process can be reflected more accurately, the reliability of monitoring data is improved, and reliable technical support is provided for desert ecosystem nutrient cycle research and ecological restoration effect evaluation.

The present application belongs to the field of microbial technology. More particularly, it relates to a salt-tolerant Bradyrhizobium sp. strain RY15 and its application. The Bradyrhizobium sp. strain RY15 provided by the present application has excellent salt tolerance and can significantly promote the growth and development and nodule fixation ability of Desmodium velutinum. The pot experiment results prove that the application of the RY15 strain can significantly increase the fresh weight of the aboveground part, the fresh weight of the underground part and the number of nodules of Desmodium velutinum; in the saline soil field, the yield of the pasture is increased by 68.89% after the inoculation of the RY15 inoculant. The RY15 strain of the present application can be made into a liquid or solid microbial inoculant, which is suitable for seed dressing, root soaking or soil application, and is simple to operate, low in cost and easy to popularize. The RY15 strain of the present application is a natural symbiotic bacterium, is safe and non-toxic, can improve the nitrogen cycle of soil, expand the planting of high-quality leguminous forage in saline-alkali soil, and has good application prospect and application value.

The present application provides a pregelatinized hydroxypropylated pea starch that has improved water-resistant film forming property compared to previously known film forming pea starches. The starch may be characterized by a RVA viscosity profile. It may be obtained by the pregelatinization of a hydroxypropylated pea starch, notably on a drum dryer. The improved water-resistance was illustrated on films immersed into water and was also characterized by the determination of the hydrophobicity value of the starch using force tensiometry, goniometry and the Young-Laplace and Fowkes model.

The present invention relates to a highly soluble legume starch having a content of less than 10%, more preferably less than 6% by weight of oligosaccharides with a degree of polymerization (DP) of 1 and 2; a content of more than 50%, more preferably more than 70% by weight of oligosaccharides with a DP of 3 to 20; a water solubility of more than 90%, more preferably more than 95% by weight; a viscosity of less than 500 cP, more preferably less than 100 cP, and characterized by an α1,4 / α1,6 ratio of between 25% and 35%, preferably between 28% and 32% determined by 13 C NMR.

This utility model discloses a handheld seeder for small-particle legume forage, including a bracket, a connecting plate, a support column, a limiting cylinder, and a limiting rod. The bracket is fixedly connected to both sides of the connecting plate, and the support column is fixedly connected to both sides of the top right side of the connecting plate. This utility model, by incorporating a handle shock-absorbing mechanism, uses the cooperation of movable shaft one, inclined rod, sliding sleeve, movable shaft two, and the limiting rod to buffer the vibration generated during seeder movement. Combined with the rubber pad covering the surface of the push rod, it effectively reduces the transmission of vibration to the operator's hand, alleviating hand fatigue and allowing the operator to more easily hold and push the seeder, improving operational comfort and stability, thereby ensuring the continuity and efficiency of the sowing operation. It also has the advantage of reducing hand fatigue during use.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust) of the plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Provided is use of MfERF026 regulation in growth, development, and stress tolerance of Medicago sativa, which belongs to the technical field of plant genetic engineering. Provided is use of a regulatory agent for MfERF026, which is an ethylene responsive factor (ERF) in Medicago falcata, in promoting growth and development and improving drought tolerance and cold tolerance of M. sativa. The inhibited expression of MfERF026, the ERF in M. falcata may enhance the drought tolerance and cold tolerance of the M. sativa. Moreover, the inhibited expression of MfERF026, the ERF in M. falcata, may regulate the growth and development of M. sativa, thereby accelerating the growth rate of M. sativa to increase the number of leaves. The inhibited expression of MfERF026 provides a novel theoretical basis and a candidate gene for cultivating novel leguminous forage varieties with high resistance, yield, and protein content.

The invention provides an intercropping method for realizing salinity regulation and control and nitrogen synergy through sophora alopecuroide-filmless cotton space configuration, and belongs to the technical field of saline-alkali soil ecological farming. Wide and narrow rows are arranged, planting is carried out alternately, sophora alopecuroide is sown in the wide row to serve as a sophora alopecuroide belt, and cotton is sown in the narrow row to serve as a cotton belt; respectively performing drip irrigation in a seedling stage and a squaring stage; and cutting and returning to the field. By adjusting the spatial configuration of the leguminous shrub sophora alopecuroides and the film-free cotton, the mulching film pollution is eradicated; sophora alopecuroides root growth can improve the soil porosity and promote salt leaching, the overground part can cover and block evaporation salt return, and the salt surface accumulation problem of the film-free cotton field is solved; sophora alopecuroides nitrogen fixation substitution can reduce nitrogen fertilizer consumption. Sophora alopecuroides green manure returning to the field can improve soil organic matters and realize synchronous improvement of carbon and nitrogen. According to the method, plant ecological niche regulation is taken as a core, the cost is low, four-dimensional collaboration of salt inhibition, nitrogen increase, pollution reduction and synergism of the saline-alkali soil cotton field can be realized, and a standardized solution is provided for agricultural green transformation in arid regions.

The present application relates to the technical field of ecological restoration, and specifically discloses a method for repairing degenerated alpine meadow bare patches by using soil-plant feedback. The method comprises the following steps: separately planting candidate species in degenerated soil and non-degenerated soil, and screening suitable complementary species for degenerated soil; grouping the screened species according to functional groups, and planting the species in degenerated soil to determine an optimized species grouping scheme for different restoration targets. Through a two-stage screening system, three efficient groupings are obtained: a pure grass combination for rapid coverage and surface stabilization, a single legume + grass combination for stress resistance and balanced restoration effect, and a high-diversity double legume combination for long-term improvement and fertilization, thereby providing a scientific and accurate grouping decision-making tool for repairing degenerated alpine meadow bare patches.