Eight cassane diterpenoid compounds having substantial antitumor activity

A technology of anti-tumor activity and compounds, applied in the field of extraction and separation, can solve problems such as no patents or literature reports

Inactive Publication Date: 2014-07-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The compounds I-VIII involved in the present invention are all new compounds, and their anti-tumor activity is discovered for the first time. So far, the above research results have not been patented or reported in the literature

Method used

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  • Eight cassane diterpenoid compounds having substantial antitumor activity
  • Eight cassane diterpenoid compounds having substantial antitumor activity
  • Eight cassane diterpenoid compounds having substantial antitumor activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Cytotoxicity test:

[0030] The activity of each compound was screened by MTT colorimetry. Select gastric adenocarcinoma (ASG), lung cancer (A549), liver cancer (HepG-2), breast cancer (MCF-7) culture medium and dilute it with 6×10 4 Inoculate in a 96-well plate at a density of / ml, 100 μl per well. After 24 hours of normal culture in the incubator, add the drug. The final concentrations of the drugs were 2.5 μg / ml (group 1), 5 μg / ml (group 2), 10 μg / ml (group 3), 20 μg / ml (group 4), and 40 μg / ml (group 5). A total of 5 concentrations were set up, with 3 replicate wells for each concentration, and a blank group was set up. After culturing for 48 hours, add 10 μl of MTT to each well for staining. After continuing to cultivate for four hours, discard the original culture solution, add 100 μl of DMSO to each well, place on a shaker and shake at low speed for 10 minutes to fully dissolve the crystals, and detect the optical density at a wavelength of 570 nm with an enzym...

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Abstract

The invention relates to eight new cassane diterpenoid compounds separated from the seed of a leguminous Caesalpinia L. plant Caesalpinia minax Hance through a phytochemical extraction separating process, cytotoxicity of the compounds on the gastric gland cancer, the lung cancer, the liver cancer and the breast cancer, and an application of the compounds in the clinic prevention and treatment of tumors.

Description

Technical field: [0001] The present invention relates to extracting and separating 8 new Kazanane-type diterpenoids and the like from the seed of Caesalpinia minax Hance through phytochemical extraction and separation means. Cytotoxic effects of the compound on gastric adenocarcinoma, lung cancer, liver cancer and breast cancer, and its application in clinical prevention and treatment of tumors. Background technique: [0002] The bitter stone lotus is the seed of Caesalpinia minax Hance in the genus Fabaceae. Commonly known as Nanshele and Dragon's Claw, it is a low shrub that grows in mountains, valleys, river valleys or roadside bushes at an altitude of 150-680m. In my country, it is mainly distributed in Guangxi and Yunnan in Southwest and South China. , Guizhou, Guangxi, Guangdong, Hainan and other places. Shilian bitterness is cool in nature, bitter in taste, has the effects of clearing away heat and dampness, promoting blood circulation and dispelling blood stasis, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/738C07C69/21C07C67/48C07D307/77A61P35/00
CPCC07C69/738C07C67/48C07C69/21C07C2603/26C07D307/77
Inventor 许旭东马国需袁经权曹丽吴海峰张小坡田瑜吴丽珍郑庆霞孙照翠钟明亮杨峻山
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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