Treatment method for increasing germination rate of medicago truncatula seeds

A treatment method and germination rate technology, which is applied in the treatment field of improving the germination rate of Medicago truncatula seeds, and achieves the effect of quick effect, low economic cost and less dosage

Inactive Publication Date: 2014-05-14
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Treatment of Medicago truncatula seeds with signal substance (NO) to study its dormancy mechanism and method of breaking dormancy is still rarely reported

Method used

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  • Treatment method for increasing germination rate of medicago truncatula seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Utilize the method for improving the germination rate of Medicago truncatula seeds by sodium nitroferricyanide, comprising the following steps:

[0015] 1) Pick plump Medicago truncatula pods, soak in water for 12 hours and then peel off the shell;

[0016] 2) Select plump, uniform-sized Medicago truncatula seeds, and use 0.2% mercury liter (HgCl 2 ) Soak the surface for disinfection for 5 minutes, and then rinse it with distilled water for 5 times;

[0017] 3) Soak the sterilized Medicago truncatula seeds in 0.1mmol / L sodium nitroferricyanide solution (SNP) for 48 hours, then place them in a petri dish (Φ=9 cm) with double-layer filter paper, 100 seeds per dish , repeated 3 times, and cultured at a temperature of 25°C, 12h of light (3000LX)+12h of darkness.

[0018] 4) For the determination of seed germination, the germination rate was counted every day with the whiteness of the seed as the germination mark, and the statistics were totaled for 7 days. The germin...

Embodiment 2

[0026] The specific implementation method is the same as that in Example 1, except that in step 2), the method is washed with distilled water for 6 times; in step 3), the light culture temperature is 24°C.

Embodiment 3

[0028] The specific implementation method is the same as that of Example 1, except that, in step 3), the temperature for light cultivation is 26°C.

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Abstract

The invention discloses a treatment method for increasing the germination rate of medicago truncatula seeds. The treatment method comprises the steps of after disinfecting the medicago truncatula seeds, soaking the medicago truncatula seeds by using a 0.1 mmol / L sodium nitroprusside solution for 48h, and culturing the medicago truncatula seeds in a dark place for 7 days at the temperature of 25+ / -1 DEG C and 12h illumination (3000LX) / 12h to promote seed germination. The treatment method for increasing the germination rate of the medicago truncatula seeds has the beneficial effects that the 0.1mmol / L sodium nitroprusside (SNP) solution is used for treating the medicago truncatula seeds so that the germination is remarkably promoted, and the germination rate reaches 62.14 percent, and is increased by 54.47 percent in comparison with those of a contrast group. Sodium nitroprusside is nontoxic at a low concentration, few in consumption amount when being used for treating the seeds, economical, short in time and high in effect taking speed; the seeds are not damaged, and the method is simple and is not influenced by factors such as season, weather, and natural hazard. By using the method, the problem of low germination rate of the medicago truncatula seeds can be solved, and germplasm resources with good germplasm are provided for biological study of legumes and production of medicago truncatula.

Description

technical field [0001] The invention relates to a seed treatment method, in particular to a treatment method for improving the germination rate of Medicago truncatula seeds. Background technique [0002] Medicago truncatula (Medicago truncatula Gaertn) It is an annual plant of the legume family Alfalfa, native to the tropics and subtropics. The plant grows prostrate, and the grass yield is low, which is a grazing type pasture. The growth period is about 150 days. The pods are spherical, with spiny hairs outside, shaped like Tribulus truncatula, also known as truncated alfalfa or truncated alfalfa. After maturity, the pods are easy to fall off, and threshing is more difficult. Quinoa alfalfa likes warm and humid climate, has strong drought tolerance, and grows well in areas with precipitation of 275-500 mm. The soil requirements are not strict, and it is suitable for neutral or alkaline soil with a pH value of 6.0 or above, and has strong adaptability to boron. Medicago...

Claims

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Application Information

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IPC IPC(8): A01C1/02
Inventor 魏小红刘文瑜龙雨曹丽赵萌许可成
Owner GANSU AGRI UNIV
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