Aschersonia aleyrodis strain and uses thereof

A technology of Ascosporium and Ascosporium, which is applied to the strain of Ascosporium whitefly and its application field, which can solve the problems of not finding the strain of Ascosporium whitefly and achieve the effect of no pollution to the environment

Inactive Publication Date: 2008-05-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prevention and control of pests such as whitefly, citrus whitefly, and aphids, traditional chemical control methods are mostly used, and in the aspect of biological pesticide control, no strains of S.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1.1 Material source

[0012] The carcass of Dialourodes citri, a citrus whitefly infested by the fungus, was collected from fruit trees in the Guangzhou area.

[0013] Potato Dextrose Agar Medium (PDA): Cook 200g potatoes to extract juice, then add 20g glucose + 17-20g agar powder to the juice, add water to boil the total volume to 1000ml. Then sterilize by autoclave (121℃, 30min).

[0014] Aseptic operating conditions: All utensils and utensils must be autoclaved (121℃, 30min), and operations such as inoculation should be performed in an ultra-clean workbench.

[0015] Cultivation conditions: culture in a light (14L:10D) incubator at 25°C. After colonies are formed, transfer to a PDA slope, and then transfer to a refrigerator at 4°C for storage.

[0016] 1.2 Isolation and identification of pathogenic bacteria

[0017] (1) Isolation and purification of pathogenic bacteria

[0018] (1) Separation

[0019] The pathogenic bacteria were isolated from the collected samples of ci...

Embodiment 2

[0057] Example 2 Determination of the pathogenicity of Bemisia tabaci against Bemisia tabaci

[0058] Bioassay is one of the effective methods to detect the lethality and lethality rate of entomogenous fungi to target pests, and can provide an important reference for comprehensive evaluation of the fungus's biological control potential. The present invention measures the pathogenicity of the Ascosporium AA01-N8 strain against Bemisia tabaci to Bemisia tabaci, so as to screen out the optimal concentration used in prevention and treatment.

[0059] Tested insects and host plants: Bemisia tabaci, the adult Bemisia tabaci raised on cucumbers in the greenhouse, inoculated on insect-free cucumber seedlings, 12 hours after laying eggs, remove the adults, and place the cucumber seedlings at 25±0.5℃ When the whitefly develops to the 2nd instar nymph, it will be used. Cucumis sativus L., a variety of Wanji Cucumber, was purchased from the Vegetable Research Institute of Guangdong Academy of...

Embodiment 3

[0071] Example 3 Determination of the pathogenicity of Ascosporium whitefly on aphids

[0072] Test insects and host plants: aphids, inoculate the aphids raised on the cabbage in the net room on the insect-free cabbage seedlings, 12h after laying eggs, remove the adults, and place the cabbage seedlings at 25±0.5℃ In the light incubator, wait until the aphids develop to the second instar larvae. Brassica campestris L. ssp. chinensis was purchased from the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences. The test seedlings were all potted seedlings with 6-8 unfolded leaves.

[0073] (1) Treatment of test strains

[0074] After purification, the AA01-N8 strain of Ascosporium candidum was cultured on a PDA plate in a thermostat (14L:10D) at 25±0.5℃ for 10 days, and 0.3% Tween-80 sterilized water was added to collect the spores. , Stir the spore suspension on a magnetic stirrer for 20 minutes. After the spores are dispersed evenly, filter the impurities with ...

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Abstract

The invention discloses an aschersonia aleyrodis strain and the application of the strain. The aschersonia aleyrodis strain is a wild strain separated from bemisia tabaci body in Guangzhou area, which is naturally infected by worm living fungus. The wild strain is multi-planted on the bemisia tabaci body for the rejuvenation, so that the aschersonia aleyrodis strain is obtained; the purified strain is obtained through single spore isolation. The paecilomyces fumosoroseus strain is AA01-N8; the preservation number of the strain is CCTCC No: M207087; the strain is stored in China Center for Type Culture Collection on 27 June, 2007. Through a long term of infectious biology research and the indoor biology testing, the aschersonia aleyrodis has strong infectious and killing effects for a plurality of sucking mouth parts insects, such as the white fly and the cotton worm etc.

Description

Technical field [0001] The invention relates to a kind of Aschersonia aleyrodis Webber, which is used for preventing and curing Bemisia tabaci, citrus whitefly, aphids and other pests on cruciferous vegetables. Background technique [0002] In the prevention and control of Bemisia tabaci, citrus whitefly, aphids and other pests, traditional chemical control methods are often used. In the prevention and control of biological pesticides, no Ascospora whitefly strains used to control the above pests have been found. This fungus is an insect pathogenic fungus. As a living biological pesticide, it has a new mechanism of action different from existing chemical pesticides, and has the characteristics of no pollution and no residue. It can be used to prevent and control certain Bacillus thuringiensis ( Bt) Alternative control drugs for insect resistant insects. Summary of the invention [0003] In order to control whitefly, citrus whitefly, aphids and other pests, the primary objective ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/00
Inventor 黄振任顺祥
Owner SOUTH CHINA AGRI UNIV
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