153 results about "Alfalfa seed" patented technology

The invention discloses an alfalfa SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The SNP molecular marker combination is composed of 61351 SNP molecular markers, the physical positions of the 61351 SNP molecular markers are determined based on sequence alignment of a medicago sativa reference genome ZM-4alfalfa gene, the SNP molecular markers are good in site specificity, high in universality and high in polymorphism, medicago sativa germplasms of different sources can be effectively distinguished, and the application prospect is wide. The alfalfa whole-genome liquid-phase gene chip prepared from the SNP molecular marker can be used for alfalfa germplasm resource genetic diversity evaluation, genetic map construction, functional gene positioning, whole-genome association analysis and the like, and provides important tools and technical supports for alfalfa molecular design breeding and variety authenticity identification. The method has important significance on high-quality development of alfalfa seed industry in China.

The invention relates to the field of alfalfa planting, in particular to a synthetic flora for breaking dormancy of alfalfa seeds and promoting growth of seedlings and application of the synthetic flora. The invention provides a strain of sinorhizobium meliloti NFB5, the preservation number is CGMCC NO.33618, and the sinorhizobium meliloti NFB5 can break dormancy of alfalfa seeds and promote growth of alfalfa seedlings and root systems. According to the method, sinorhizobium meliloti NFB5 is compounded with one or two of screened salt-tolerant brevibacillus brevis EB3 and bacillus PS06 to obtain a synthetic flora, and alfalfa seeds are soaked in a bacterial solution containing the synthetic flora, so that the number of hard seeds can be remarkably reduced, the germination rate and germination potential of the seeds can be remarkably improved, and the germination rate of the seeds can be remarkably increased. Therefore, the synthetic flora can well break the dormancy of the alfalfa seeds, does not cause loss to the seeds, and does not reduce the quality of the seeds. In addition, the synthetic flora also has a significant growth promoting effect, and can significantly improve the plant height, root length, stem weight and root weight of seedlings.

The invention discloses an accurate water and fertilizer regulation and control technology for an alfalfa seed field, and aims to improve the water and fertilizer utilization efficiency and seed yield of alfalfa by optimizing a water and fertilizer management strategy. The invention provides a precise regulation and control mode based on irrigation amount and fertilization amount coupling, the evaporation amount of a reference crop is calculated by using a Penman-Monteith formula, the optimal water and fertilizer ratio suitable for different alfalfa growth stages is provided based on a pot experiment and a field experiment, a comprehensive evaluation system is constructed, an economic benefit analysis model is combined, and the optimal water and fertilizer ratio is calculated. A scientific basis is provided for popularization and application. According to the technology, the growth indexes (such as plant height and leaf area) of alfalfa are remarkably improved, and the seed quality (such as thousand seed weight and germination rate) is improved. In addition, efficient utilization and sustainable development of resources are achieved, and remarkable economic and ecological benefits are achieved.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

The invention belongs to the technical field of agricultural biology and soil remediation, and particularly relates to a mining area soil remediation method. The method comprises the following steps: performing soil reconstruction on a mining area to obtain reconstructed soil with a multi-layer structure; the reconstructed soil of the multi-layer structure sequentially comprises a reconstruction layer, a blocking layer and a filling layer from top to bottom. The reconstruction layer comprises low-carbon coal gangue and surface soil, the particle size of the low-carbon coal gangue is smaller than or equal to 8 mm, and the mass ratio of the low-carbon coal gangue to the surface soil is (1-2): (1-2); the blocking layer comprises a grouting mixture of fly ash and cement, and the mass ratio of the fly ash to the cement is smaller than or equal to 1.8: 1; the filling layer comprises blocky waste coal gangue; and then planting alfalfa on the reconstructed soil with the multilayer structure. According to the method, recycling of the waste coal gangue and reconstruction of the mining area soil are realized, so that smooth germination and healthy growth of alfalfa seeds on the reconstructed soil are ensured, mining area soil remediation is realized, and an economical and feasible method is provided for mining area soil remediation.

The invention relates to the technical field of watering control, in particular to an intelligent watering control method for alfalfa seed production, which comprises the following steps: collecting humidity data of current soil through a soil humidity sensor, analyzing a soil humidity state and generating a soil humidity result; evaluating the required water quantity according to the soil humidity result, and adjusting the output quantity of a water pump to obtain an adjusted watering plan. The current soil humidity state of the farmland is obtained by monitoring the soil humidity in real time, so that the output quantity of the water pump is dynamically adjusted, intelligent regulation and control of the watering plan are achieved, the irrigation accuracy is improved, and waste and insufficiency of water resources in the watering process are avoided; meanwhile, the watering plan is optimized by means of air temperature and rainfall data, so that the irrigation action is closely combined with the actual weather condition, the irrigation timeliness is improved, and the negative effects caused by adverse weather conditions are reduced.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The invention discloses a method for identifying and counting alfalfa seed pods with different maturity degrees based on RHE-DETR, and belongs to the technical field of target detection. The method comprises the following steps: acquiring alfalfa seed pod images under different illumination conditions and shielding conditions in a field; labeling software is used for labeling the collected images, and an alfalfa seed pod data set is constructed and divided; the model is improved, and a lightweight target detection model RHE-DETR for a small target is obtained; inputting the constructed alfalfa seed pod data set into an RHE-DETR target detection model for training to obtain a weight file of the model after the alfalfa seed pod data set is trained; and deploying a weight file trained by the RHE-DETR target detection model to an edge computing platform to detect a field alfalfa seed pod image to obtain a real-time tracing frame, confidence and counting result of the alfalfa seed pod. According to the method, the conditions of error detection and missing detection in a complex field environment are reduced, the accuracy of identifying and counting the maturity of the alfalfa seed pods is improved, and meanwhile, the identifying and counting time is also shortened.

The invention relates to the technical field of ecological planting, in particular to a planting method for improving the biological activity of citrus orchard soil by interplanting california burclover. The planting method comprises the following steps: (1) sowing california burclover seeds in a citrus garden in late October; (2) when the california burclover enters a branching period, applying a fertilizer; (3) naturally decomposing after the california burclover plant is withered; the california burclover seeds naturally fall into the soil. According to the planting method, the fertility of soil in the citrus orchard can be improved, water and soil are kept, microclimate is adjusted, and environmental pollution is reduced; production cost is reduced, and extra income is increased; the income increase of farmers is promoted. And a basis is provided for ecological and sustainable development of the citrus orchard.

The invention discloses an alfalfa planting management method based on high-altitude saline-alkali soil, and relates to the technical field of agricultural cultivation, and the method comprises the following steps: S1, an irrigation water preheating step: enabling irrigation water to flow through a deep-color pipeline laid on the ground surface for solar preheating; the preheated irrigation water is used for irrigating the field in a drip irrigation mode, and the irrigation water amount is determined according to the initial salt content of the soil and the target salt leaching depth. According to the alfalfa planting management method based on the high-altitude saline-alkali soil, through combination of irrigation water preheating treatment and precise drip irrigation, the contradiction between salt leaching operation and ground temperature keeping in a high-altitude area is effectively solved. Preheated irrigation water avoids soil temperature sudden drop caused by cold water irrigation, the layered covering technology synergistically inhibits water evaporation and salt upward returning, meanwhile, the soil temperature is kept stable, a proper water temperature and salt environment is created for alfalfa seed germination, and therefore the seedling emergence uniformity and the seedling survival rate are improved.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention discloses an alfalfa seed threshing device and a using method thereof, the alfalfa seed threshing device comprises an alfalfa seed threshing shell, a first movable alfalfa seed threshing plate and a second movable alfalfa seed threshing plate, and the right upper end of an inner cavity of the alfalfa seed threshing shell is movably connected with driving alfalfa seed crushing teeth. According to the alfalfa seed threshing device, the motor, the first elliptical wheel, the driving alfalfa seed crushing teeth and the driven alfalfa seed crushing teeth are arranged, alfalfa seeds to be threshed are put into the alfalfa seed threshing shell from the feeding opening, the motor is started, and the first elliptical wheel can be driven to rotate through the single-face tooth synchronous belt; the first elliptical wheel can drive the driving alfalfa seed crushing teeth to rotate through the single-face tooth synchronous belt while rotating, the driving alfalfa seed crushing teeth can drive the driven alfalfa seed crushing teeth to rotate while rotating, and therefore alfalfa seeds can be crushed.

The invention relates to a traditional Chinese medicine composition for treating or preventing osteoporosis, and discloses application of the traditional Chinese medicine composition in preparation of a medicine for treating and / or preventing osteoporosis. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight: 22-26 parts of bull penis, 8-12 parts of cinnamon, 10-14 parts of alfalfa seeds, 8-12 parts of galangal, 10-14 parts of onion seeds, 8-12 parts of clove, 10-14 parts of fructus ulmi, 8-12 parts of pepper, 12-16 parts of carrot seeds, 38-46 parts of sesame, 10-14 parts of muskmelon seeds, 56-64 parts of almonds, 10-14 parts of cucumber seeds, 8-12 parts of asarum, 10-14 parts of semen allii tuberosi and 16-20 parts of limonium macrophyllum. The medicine is prepared from the following raw materials in parts by weight: 10-14 parts of dill seeds, 13-17 parts of tribulus terrestris, 36-44 parts of milk peaches, 8-12 parts of mirabilis jalapa roots, 18-22 parts of cotton seeds, 10-14 parts of radix aucklandiae, 10-14 parts of ginger slices, 8-12 parts of nutmeg coats, 46-54 parts of pinus bungeana, 10-14 parts of sesame seeds, 3-7 parts of saffron, 8-12 parts of fern, 8-12 parts of fructus piperis longi and 18-22 parts of nutmeg.

The invention discloses an alfalfa pod shell rapid smashing device, and relates to the technical field of smashing devices, the alfalfa pod shell rapid smashing device comprises a fixing plate, a rack, a lifting unit, a grinding bin, a grinding plate, a spiral through groove and an extrusion unit; the lifting plate is driven by the extrusion unit to ascend and descend; a motor; and the rotating pressing disc is located over the grinding plate, and the outer diameter of the rotating pressing disc is smaller than that of the grinding plate. According to the device disclosed by the invention, the spiral part of an alfalfa pod is clamped into the spiral through groove, so that the rotary pressure plate generates shearing force on the hull of the alfalfa pod, the hull of the alfalfa pod is split, seeds in the pod are separated from the hull, and meanwhile, the seeds are relatively small in size and fall onto the fixed plate from the spiral through groove; and then the lifting unit drives the grinding bin to move upwards, so that the extrusion force of the capsule shells of the fruit pods, which is applied by the rotary pressing disc, is increased, and along with the continuous rotation of the rotary pressing disc, the capsule shells of the fruit pods can be ground, so that the capsule shells are ground and crushed.

The invention discloses a growth-promoting streptomyces strain with saline-alkaline tolerance and application thereof, and belongs to the technical field of microorganisms. In order to excavate streptomyces capable of improving the saline-alkali stress resistance of alfalfa, a strain of Streptomyces Beauvianus Jrh8-9 is screened from rhizosphere soil of ranunculus japonicus growing in saline-alkali soil, and a saline-alkali tolerance experiment, a germination accelerating experiment and a potting experiment prove that the Streptomyces Beauvianus can survive and grow in a high-salt and high-pH environment, and the Streptomyces Beauvianus Jrh8-9 has the advantages that the Streptomyces Beauvianus Jrh8-9 can be used for improving the saline-alkali stress resistance of alfalfa; and germination of alfalfa seeds and growth of alfalfa seedlings under saline-alkali stress can be promoted. In addition, under the non-stress condition, the strain Jrh8-9 also has a certain promotion effect on the growth of alfalfa seedlings. The invention provides a valuable strain resource for promoting the growth of alfalfa under saline-alkali stress.

The invention relates to the technical field of seed treatment, and provides a pelleting method for alfalfa seeds used in a coastal saline-alkali district, and the alfalfa seeds sequentially comprise seeds, a hormone layer, a first oxygenation breathable layer, a nutrition layer, a second oxygenation breathable layer and an outer protection layer from inside to outside; the raw material of the hormone layer comprises gibberellin; the raw materials of the first oxygen-increasing breathable layer comprise an adhesive and a functional material; the raw material of the second oxygenation breathable layer comprises a functional material; the functional material comprises gypsum and / or calcium peroxide; the raw material of the outer protection layer comprises organic acid. By means of the technical scheme, the problem that the emergence rate of alfalfa seeds is low due to the fact that saline-alkali soil in a saline-alkali area is large in viscosity and poor in air permeability in the prior art is solved.

The invention discloses a seed treatment method for improving seed germination and planting efficiency of medicago sativa under drought conditions, and belongs to the field of seed treatment. According to the method for promoting seed germination, novel carbon nano quantum dots are used as an initiator to treat medicago sativa seeds; after the alfalfa seeds are subjected to initiation treatment, the drought resistance of the alfalfa seeds can be remarkably improved, seed germination and seedling growth can be promoted, the germination rate can be increased by 23%, the germination potential can be increased by 34%, the plant height can be increased by 90%, the biomass can be increased by 70%, meanwhile, the antioxidant enzyme activity can be remarkably improved by 33.1%, and the malondialdehyde content can be remarkably reduced by 26.43%. By applying the seed priming method, the planting efficiency can be improved, and meanwhile, the planting cost is reduced.

The invention discloses an application of an RH1 gene or an encoding protein thereof in regulation and control of physical dormancy of plants. The RH1 gene capable of regulating the physical dormancy of the plant is separated from medicago truncatula, the RH1 gene is over-expressed in the medicago truncatula, transgenic medicago truncatula is obtained, and compared with wild medicago truncatula, seeds of the transgenic medicago truncatula show phenotypes of water swelling, seed germination rate increase and the like, and the transgenic medicago truncatula has the advantages that the plant physical dormancy can be regulated and controlled; the physical dormancy of the transgenic medicago truncatula seeds is obviously broken, and the RH1 gene or the encoded protein thereof is proved to have the function of regulating the physical dormancy of the plant. The method has application prospects in the aspects of promoting plant seed germination, reducing the production cost during seed germination and the like.

The invention discloses a method for increasing the yield of alfalfa seeds by using a growth regulator. The method comprises the following steps: (1) selecting a seed field production area; (2) land parcel selection; (3) soil pretreatment before sowing; (4) sowing; (5) field management; (6) harvesting and drying; in the step (5), a compound sodium nitrophenolate solution is uniformly sprayed in the bud stage, the dosage is 200 L / hm < 2 >, and the mass concentration of the compound sodium nitrophenolate solution is 5.0-6.0 ppm. The hole sowing machine is adopted to accurately control the sowing depth and the sowing quantity, the sowing density of an alfalfa seed field is strictly controlled, the optimal plant growth regulator is innovatively matched for application, and the alfalfa seed yield is effectively improved.

According to the stress-resistant agent for alfalfa planting and the use method of the stress-resistant agent, the synergistic effect of betaine, glucose and monopotassium phosphate is utilized, and a seed soaking or foliage spraying mode is adopted, so that on one hand, expression of a series of stress-resistant related genes can be induced, ROS generated by stress excitation can be eliminated, and the stress resistance of alfalfa can be improved; the damage of reactive oxygen free radicals and the like to plasma membranes is reduced, the activity of antioxidant enzymes is enhanced, the accumulation of osmotic regulation substances is enhanced, and the chlorophyll synthesis of alfalfa leaves is remarkably improved, so that the stress resistance of plants is enhanced, and the damage of adversity stress to alfalfa plants is relieved. The stress-resistant agent is simple in component, free of pungent smell and good in stress-resistant effect, has the characteristics of no environmental pollution, zero emission and no residue, and can obviously promote germination of alfalfa seeds, regulate growth of alfalfa seedlings and improve stress resistance of the alfalfa seedlings.

The invention discloses a mixed planting method for alfalfa and wheat. The alfalfa and wheat mixed planting method comprises the following steps that alfalfa seeds are taken and subjected to skin breaking and coating treatment, and pelletized alfalfa seeds are obtained for standby application; performing deep ploughing, soil preparation and base fertilizer application on the land parcels to be sown, using an underground drip irrigation pipe laying machine to lay underground drip irrigation belts with the burial depth of 15-20 cm, and covering soil on the earth surface by 8-10 cm; a proper amount of pelletized alfalfa seeds and wheat seeds are mixed and sown, first-time drip irrigation is carried out after sowing, and second-time drip irrigation is carried out after seedling emergence; cutting is carried out twice in the same year of planting, drip irrigation is carried out before and after each time of cutting, and the irrigation amount before cutting is 30-40% of the total irrigation amount of the stubble. According to the alfalfa and wheat mixed planting method, the emergence rate of alfalfa seeds and the survival rate of alfalfa seedlings can be greatly increased, the seed sowing amount and the watering amount are reduced, and the alfalfa planting cost is reduced.

The invention relates to the technical field of alfalfa cultivation, in particular to an alfalfa seed tedding device which comprises a machine body, a feeding hopper and a discharging port. An output shaft of a servo motor can drive a reciprocating lead screw to rotate, the reciprocating lead screw penetrates through and is in threaded connection with a reciprocating sliding sleeve, and the reciprocating sliding sleeve is in sliding connection with the machine body; due to the fact that the reciprocating screw rod drives the C-shaped frame to move in a reciprocating mode through the reciprocating sliding sleeve when the reciprocating screw rod rotates, and the C-shaped frame drives the scraping plate to move together through the telescopic rod, the first sliding rods fixedly connected to the two sides of the scraping plate are inserted into the inclined grooves, and the inclination angle of the inclined grooves is the same as that of the drying plate. According to the clover seed tedding device, the inclined groove is formed in the bottom of the scraping plate, the scraping plate can drive the first sliding rod to slide along the inclined groove when moving, the first sliding rod can drive the scraping plate to enable the bottom face of the scraping plate to be attached to the surface of the airing plate all the time, and clover seeds can be scraped back and forth by the scraping plate and evenly spread on the airing plate through back-and-forth movement of the scraping plate, so that the clover seeds are tedded more evenly.

The invention relates to the technical field of biological enzymes, and discloses serine protease as well as a preparation method and application thereof. Wherein the amino acid sequence of the serine protease is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The serine protease provided by the invention has stable activity at different temperatures, can better maintain the activity and efficacy in practical application, and can still effectively inhibit the formation of a biological membrane or remove a mature biological membrane under the condition of variable temperatures. Experimental results show that the serine protease can obviously inhibit the formation of the methicillin-resistant staphylococcus aureus biological membrane and remove the methicillin-resistant staphylococcus aureus biological membrane; no antibacterial activity is found, so that pathogenic bacteria are not easy to generate drug resistance; meanwhile, toxicity is avoided, and the germination rate and bud length of alfalfa seeds are not affected. Therefore, the serine protease is safe and reliable in application in the food industry, and has great advantages in food application.

The invention relates to the technical field of seed pelleting processing, in particular to an alfalfa seed pelleting material and a preparation method of alfalfa seed pellets. Through scientific compatibility and synergistic effects in a pill coating material system, the water-retaining agent guarantees a moisture basis, the microbial agent constructs a biological barrier and promotes conversion, the inorganic nutrients provide material support, and the bioactive substances stimulate intrinsic potentials and synergistically act on the alfalfa seed germination physiological process, so that the alfalfa seed germination rate is increased, and the alfalfa seed germination rate is increased. Finally, the technical effects of remarkably promoting the germination rate and uniformity of the seeds and improving the nodulation rate are achieved, and a solid foundation is laid for developing high-quality and high-yield alfalfa seeds.

The utility model discloses an alfalfa seed germination promoting device and relates to the technical field of seed processing equipment. The device comprises a supporting pipe, a filter cartridge is arranged at the bottom of the supporting pipe, and a preheating tank is arranged at the bottom of the filter cartridge; a shelling assembly is arranged on one side of the supporting pipe and comprises a cooling pipe, the bottom of the cooling pipe communicates with a compression refrigerating machine, a transmission rod is arranged in the cooling pipe, the two sides of the surface of the transmission rod are both sleeved with isolation plugs, and a heater is fixedly connected to the interior of the preheating tank. And the seeds are hulled through the hulling assembly. According to the alfalfa seed hulling device, through the arrangement of the hulling assembly, seeds can be subjected to freezing treatment and warm water soaking treatment respectively, shrinkage and expansion of shells on the surfaces of the seeds can be promoted through the alternate cooling and heating treatment process, shell breaking is facilitated, and the germination rate of the alfalfa seeds is increased; the seed processing position can be adjusted, and the processing efficiency is improved.

The invention belongs to the technical field of agricultural planting, and provides a method for interplanting triticale and alfalfa in barren land, which comprises the following steps: S1, selecting triticale and alfalfa varieties suitable for local environment; s2, in the period from September to October, soil deep ploughing is conducted on the planting land parcel by 25-30 cm, and triticale seeds and alfalfa seeds are evenly mixed according to the proportion of 7: 1; s3, harvesting triticale in different periods according to utilization purposes, and continuing to reserve alfalfa for growth after harvesting; s4, performing irrigation, disease and pest monitoring, fertilization, weeding and cutting management on the feed triticale and alfalfa; according to the method, crop varieties suitable for the local environment are selected, soil deep ploughing and seed treatment are conducted, reasonable sowing and fertilization are conducted, and fine field management measures are taken, so that efficient utilization of the barren land is achieved; according to the method, the utilization rate of land and the yield of crops are increased, diseases and insect pests are reduced through interplanting and fine management, the soil structure is improved, and the soil fertility is improved.

The present invention discloses a SNP molecular marker combination of alfalfa and its application. The SNP molecular marker combination consists of 61,351 SNP molecular markers. The physical positions of the 61,351 SNP molecular markers are determined by sequence alignment based on the alfalfa reference genome ZM-4 alfalfa genome. The SNP molecular marker sites of the present invention have good site specificity, strong universality, and high polymorphism, and can effectively distinguish alfalfa germplasms from different sources. Preparing the SNP molecular markers of the present invention into a whole-genome liquid gene chip for alfalfa can be used for genetic diversity assessment of alfalfa germplasm resources, construction of genetic maps and functional gene mapping, genome-wide association analysis, etc., providing important tools and technical support for molecular design breeding of alfalfa and identification of variety authenticity, and having important significance for the high-quality development of the alfalfa seed industry in China.

The invention provides a silk fibroin salt-resistant film as well as a preparation method and application thereof, and relates to the technical field of biological materials, and the silk fibroin biomass salt-resistant film provided by the invention comprises silk fibroin, allantoin and sodium alginate. The silk fibroin biomass salt-resistant film can be used for seed coating, the water absorption rate and the germination rate of alfalfa seeds can be remarkably improved under normal conditions, the early water absorption capacity and the germination rate of the seeds can be enhanced under salt stress conditions, and damage of salt stress to the seeds is relieved. Important theoretical basis and practical guidance are provided for improving germination and growth of the seeds in the saline-alkali soil.

The invention discloses a training method of a model for identifying the maturity of alfalfa seeds and an identifying method for the maturity of the alfalfa seeds. The training method comprises the following steps: acquiring multispectral cube image data; obtaining a prediction result output by the to-be-trained model; performing first-stage training on the to-be-predicted model; respectively obtaining feature distribution of different feature layers of the models of the source domain and the target domain after the first-stage training, and calculating the difference between the source domain and the target domain; on the basis of the difference between the source domain and the target domain, calibrating the layer statistics of the distribution characteristics of the recorded data in the model after the first-stage training to obtain a calibrated model; unfreezing a part of layers of the calibrated model, selecting hyper-parameters, and performing second-stage training on the calibrated model through a target domain to obtain the model for identifying the maturity of the alfalfa seeds. According to the method, the model capable of accurately identifying the alfalfa seed maturity can be trained, the alfalfa seed maturity can be losslessly identified based on the trained model, and the identification accuracy is high.