80 results about "Alfalfa seed" patented technology

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The invention discloses an alfalfa planting management method based on high-altitude saline-alkali soil, and relates to the technical field of agricultural cultivation, and the method comprises the following steps: S1, an irrigation water preheating step: enabling irrigation water to flow through a deep-color pipeline laid on the ground surface for solar preheating; the preheated irrigation water is used for irrigating the field in a drip irrigation mode, and the irrigation water amount is determined according to the initial salt content of the soil and the target salt leaching depth. According to the alfalfa planting management method based on the high-altitude saline-alkali soil, through combination of irrigation water preheating treatment and precise drip irrigation, the contradiction between salt leaching operation and ground temperature keeping in a high-altitude area is effectively solved. Preheated irrigation water avoids soil temperature sudden drop caused by cold water irrigation, the layered covering technology synergistically inhibits water evaporation and salt upward returning, meanwhile, the soil temperature is kept stable, a proper water temperature and salt environment is created for alfalfa seed germination, and therefore the seedling emergence uniformity and the seedling survival rate are improved.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention relates to the technical field of seed treatment, and provides a pelleting method for alfalfa seeds used in a coastal saline-alkali district, and the alfalfa seeds sequentially comprise seeds, a hormone layer, a first oxygenation breathable layer, a nutrition layer, a second oxygenation breathable layer and an outer protection layer from inside to outside; the raw material of the hormone layer comprises gibberellin; the raw materials of the first oxygen-increasing breathable layer comprise an adhesive and a functional material; the raw material of the second oxygenation breathable layer comprises a functional material; the functional material comprises gypsum and / or calcium peroxide; the raw material of the outer protection layer comprises organic acid. By means of the technical scheme, the problem that the emergence rate of alfalfa seeds is low due to the fact that saline-alkali soil in a saline-alkali area is large in viscosity and poor in air permeability in the prior art is solved.

The invention discloses a method for increasing the yield of alfalfa seeds by using a growth regulator. The method comprises the following steps: (1) selecting a seed field production area; (2) land parcel selection; (3) soil pretreatment before sowing; (4) sowing; (5) field management; (6) harvesting and drying; in the step (5), a compound sodium nitrophenolate solution is uniformly sprayed in the bud stage, the dosage is 200 L / hm < 2 >, and the mass concentration of the compound sodium nitrophenolate solution is 5.0-6.0 ppm. The hole sowing machine is adopted to accurately control the sowing depth and the sowing quantity, the sowing density of an alfalfa seed field is strictly controlled, the optimal plant growth regulator is innovatively matched for application, and the alfalfa seed yield is effectively improved.

The invention relates to the technical field of alfalfa cultivation, in particular to an alfalfa seed tedding device which comprises a machine body, a feeding hopper and a discharging port. An output shaft of a servo motor can drive a reciprocating lead screw to rotate, the reciprocating lead screw penetrates through and is in threaded connection with a reciprocating sliding sleeve, and the reciprocating sliding sleeve is in sliding connection with the machine body; due to the fact that the reciprocating screw rod drives the C-shaped frame to move in a reciprocating mode through the reciprocating sliding sleeve when the reciprocating screw rod rotates, and the C-shaped frame drives the scraping plate to move together through the telescopic rod, the first sliding rods fixedly connected to the two sides of the scraping plate are inserted into the inclined grooves, and the inclination angle of the inclined grooves is the same as that of the drying plate. According to the clover seed tedding device, the inclined groove is formed in the bottom of the scraping plate, the scraping plate can drive the first sliding rod to slide along the inclined groove when moving, the first sliding rod can drive the scraping plate to enable the bottom face of the scraping plate to be attached to the surface of the airing plate all the time, and clover seeds can be scraped back and forth by the scraping plate and evenly spread on the airing plate through back-and-forth movement of the scraping plate, so that the clover seeds are tedded more evenly.

The invention relates to the technical field of seed pelleting processing, in particular to an alfalfa seed pelleting material and a preparation method of alfalfa seed pellets. Through scientific compatibility and synergistic effects in a pill coating material system, the water-retaining agent guarantees a moisture basis, the microbial agent constructs a biological barrier and promotes conversion, the inorganic nutrients provide material support, and the bioactive substances stimulate intrinsic potentials and synergistically act on the alfalfa seed germination physiological process, so that the alfalfa seed germination rate is increased, and the alfalfa seed germination rate is increased. Finally, the technical effects of remarkably promoting the germination rate and uniformity of the seeds and improving the nodulation rate are achieved, and a solid foundation is laid for developing high-quality and high-yield alfalfa seeds.

The invention discloses a training method of a model for identifying the maturity of alfalfa seeds and an identifying method for the maturity of the alfalfa seeds. The training method comprises the following steps: acquiring multispectral cube image data; obtaining a prediction result output by the to-be-trained model; performing first-stage training on the to-be-predicted model; respectively obtaining feature distribution of different feature layers of the models of the source domain and the target domain after the first-stage training, and calculating the difference between the source domain and the target domain; on the basis of the difference between the source domain and the target domain, calibrating the layer statistics of the distribution characteristics of the recorded data in the model after the first-stage training to obtain a calibrated model; unfreezing a part of layers of the calibrated model, selecting hyper-parameters, and performing second-stage training on the calibrated model through a target domain to obtain the model for identifying the maturity of the alfalfa seeds. According to the method, the model capable of accurately identifying the alfalfa seed maturity can be trained, the alfalfa seed maturity can be losslessly identified based on the trained model, and the identification accuracy is high.

The application discloses a method for repairing heavy metal contaminated soil by combining plants and microorganisms, and comprises the following steps: planting alfalfa seeds in heavy metal contaminated soil; inoculating lead and zinc copper resistant bacteria L1 into the heavy metal contaminated soil after 30 days when the seeds germinate; and harvesting the alfalfa after the growth period of the alfalfa ends. The application has a very obvious effect on adsorbing lead, zinc and copper heavy metals in the soil. The plant raw material is low in cost, the preparation process is simple and easy to operate, and the bacterial liquid is easy to culture. The application has small investment for removing heavy metals and no secondary pollution.

The invention discloses an alfalfa seed preliminary cleaning and shelling processing device in the technical field of alfalfa seed processing, which comprises a preliminary cleaning and shelling module and a dust suction module mounted on the preliminary cleaning and shelling module, and further comprises a suspension type screening unit arranged below a discharge port of the preliminary cleaning and shelling module, the suspension type screening unit is used for bearing alfalfa materials subjected to preliminary cleaning and conducting secondary screening, swing supporting assemblies are symmetrically arranged on the two sides of the suspension type screening unit, and the swing supporting assemblies are used for movably suspending the suspension type screening unit on the equipment frame; the eccentric linkage module is in transmission connection with a driving shaft of the preliminary cleaning shelling module, and the eccentric linkage module is used for driving the suspension type screening unit to do reciprocating arc swing with the swing supporting assembly as a support; the problems that according to an existing alfalfa preliminary cleaning device, due to the fact that screen holes are fixed, fibers are wound and blocked, shelling is not thorough due to the fact that a kneading effect is lacked, and the seed loss rate is high due to unclear material layering are solved.

The invention discloses a method for accelerating drying and dehydration of alfalfa, and belongs to the technical field of alfalfa seed breeding. The method comprises the following steps that 40-50 days after the full-bloom stage of alfalfa, 50-150 mL of 30% nitra.nicotian.atrazine dispersible oil suspending agent is used per mu, 25-35 L of water is added, and leaf spraying is conducted. According to the method provided by the invention, the drying ripening agent is sprayed before alfalfa seeds are harvested, so that green leaves of alfalfa are withered and yellow and fall off, the seeds are relatively consistently ripe, the defoliation rate and the relative water loss rate of stems and leaves of alfalfa are remarkably increased, the drying time of alfalfa is shortened, and the seed yield and the germination rate are increased.

The invention discloses a production regulator for planting alfalfa and a mixing device for preparing the production regulator, belongs to the technical field of plant growth regulators, and aims to solve the problems of more raw materials and higher cost of the conventional alfalfa growth regulators. The gibberellic acid, the diethyl aminoethyl hexanoate and the brassinolide in the production regulator can effectively promote growth and development of the alfalfa, so that the alfalfa can reach a higher growth level in a short time, the amino acid water-soluble fertilizer provides rich nutrition for the alfalfa, healthy growth and yield increase of the alfalfa are facilitated, and after the production regulator is used, the growth rate of the alfalfa is increased. The yield of the alfalfa can be predicted to be increased by 10%-15%, the production regulator is simple in formula, low in cost and simple in use method, the diluted regulator only needs to be uniformly sprayed on leaves of the alfalfa when the alfalfa grows to about 30 cm, and the economic value can be remarkably increased.

The present application relates to the technical field of alfalfa planting, and discloses a phosphorus application quota distribution method for improving alfalfa seed yield, and the specific process is as follows: first, preparing the planting plot, cleaning the plot weeds in advance, leveling the land, installing the underground drip irrigation system, then, sowing and applying base fertilizer, applying the base fertilizer when finishing the land, planting the test variety by using the wide-narrow row mulching sowing method, uniformly dripping seedling water the next day to ensure uniform seedling emergence, setting the total irrigation amount and the total phosphorus application amount per hectare, and through the water and phosphorus management mode of implementing irrigation and phosphorus application quota distribution, the water and phosphorus are proportionally controlled to carry out underground drip irrigation in the growth periods of the branching stage, the squaring stage, the flowering stage and the podding stage of alfalfa, the technical scheme provided by the present application realizes the synergistic effect of differentiated irrigation amount and phosphorus application amount quota distribution in different growth periods through setting different irrigation and phosphorus application quota distribution modes, and thus improves the alfalfa seed yield and the water and phosphorus utilization efficiency.

The utility model relates to the technical field of alfalfa seed impurity removing and screening, and discloses an alfalfa seed impurity removing and screening device which comprises a first screening mechanism, and the bottom of the first screening mechanism is fixedly connected with a second screening mechanism. The first screening mechanism comprises a first screening box, a funnel is arranged at the top of the first screening box, blowing equipment is installed on the left side of the first screening box, a ventilation opening is formed in the right side of the first screening box, and a ventilation net frame is fixedly connected to the right side of the first screening box. According to the alfalfa seed impurity removing and screening device, alfalfa seeds evenly fall down, light impurities in the alfalfa seeds are blown to the right side through the blowing equipment, blown into the ventilation screen frame through the ventilation openings and fall down from the bottom of the ventilation screen frame, and then the situation that the pouring amount cannot be controlled, for example, too many alfalfa seeds are poured at a time; therefore, the problem that the alfalfa seeds with the weight lower than that in an ideal state cannot be effectively blown out by the air blowing equipment is solved.

The invention discloses alfalfa seed propagation equipment and method, and belongs to the field of leguminous plant planting, the alfalfa seed propagation equipment comprises a base and further comprises a diffusion assembly, the diffusion assembly comprises a fixing plate fixedly installed on the upper portion of the base, and a discharging frame is fixedly installed on the lower portion of the base; a discharging opening communicated with the discharging frame is formed in the fixing plate, a movable cavity is formed in the inner cavity wall of the discharging opening, a gear ring is rotationally installed in the movable cavity, a partition plate is fixedly installed on the inner wall of the gear ring, and a connecting rod is fixedly connected to the bottom of the partition plate; according to the alfalfa seeding device, alfalfa seeds can be uniformly arranged in seeding pits, and the situation that the alfalfa seeds are seeded too densely to cause intense competition and influence the growth and development of alfalfa can be avoided, so that the consistency of the spacing between planted plants can be effectively improved, and the growth of the alfalfa and the balance of light, nutrients and moisture can be effectively improved.

The invention belongs to the technical field of seed treatment, and relates to a method for improving nickel stress resistance of alfalfa seeds and application of the method. The invention provides a method for improving nickel stress resistance of medicago sativa seeds, which comprises the following steps: (1) seed disinfection: selecting full medicago sativa seeds with uniform size, soaking the medicago sativa seeds with 75% ethanol and 5% NaClO in sequence, and flushing the medicago sativa seeds with sterile water until the medicago sativa seeds are odorless; (2) stress treatment: placing the disinfected seeds in a culture dish paved with double layers of filter paper, and adding a treatment solution to enable the nickel ion concentration to be 100-500mg / L; (3) germination culture: culturing at 25 DEG C for 10 days, and maintaining humidity by a weighing method every day to complete seed germination. According to the method, the stress resistance of the alfalfa seeds under nickel stress can be remarkably improved.

The application discloses a gene for improving self-pollination seed setting rate of alfalfa and application of the gene. MsFER Experiments prove that the self-pollination seed setting rate can be improved by reducing the expression amount of a gene MsFER coding protein MsFER in alfalfa. MsFER The amino acid sequence of the protein MsFER is shown as SEQ ID No. 1. MsFER The nucleotide sequence of the gene is shown as SEQ ID No. 2. The application has important economic value and application value for improving the self-pollination seed setting rate of alfalfa seeds.

The utility model relates to the technical field of seed culture equipment, and discloses an alfalfa seed germination culture device which comprises a culture mechanism, and a rotating mechanism is arranged in the culture mechanism. When the alfalfa seed germination culture device is used, a motor is started to drive a rotating rod to rotate and drive a fixed rotating shell to rotate, a sliding block is in sliding fit with a sliding groove, the rotating range of the rotating shell is limited, stable and accurate rotation of the rotating shell is ensured, a funnel is arranged, water in a water outlet hole flows to a water discharging groove through a water discharging opening in the bottom of the funnel, and meanwhile the design of a slide is utilized; the shape of the water discharging groove is in the shape of the motion trail of the water discharging opening, so that the water can be smoothly discharged into the drawer when the rotating mechanism rotates, and the design of the device effectively solves the problem that a female worker may be inconvenient to use due to insufficient strength, and the inconvenience that the box cover needs to be taken out during culture operation. Through the design, the practicability and convenience of the device are improved.

To address the current problems of large alfalfa seed harvest losses and low purity, the present invention proposes a transversely rotating combing and detangling drum-pulling machine—a negative pressure airflow alfalfa seed collection platform. The platform comprises a press nose, a bracket assembly, an inner liner, a transmission device, a hydraulic device, a seed transport device, a combing and detangling drum, a feed auger, and a deposition area ramp. The press nose at the front end of the device presses down the plants, causing the bent alfalfa plants, the combing and detangling drum, the deposition area ramp, and the inner liner to form a relatively sealed system. Simultaneously, the combing and detangling drum rotates at high speed to generate a negative pressure rotating airflow that absorbs the alfalfa seeds detangled by the combing and detangling plate teeth into the inner cavity of the collection platform, achieving efficient collection. The combing and detangling plate teeth have a unique tooth shape, forming circular detangling holes between the teeth, allowing detangling from bottom to top along the plant's growth direction, achieving excellent detangling effects.

The invention discloses an alfalfa seed specific expression promoter and application thereof in rapid screening and identification of transgenic plants. The invention belongs to the technical field of biology, and particularly relates to an alfalfa seed specific expression promoter and application thereof in rapid screening and identification of transgenic plants. The promoter is a DNA molecule of a), b) or c) as follows: a) a DNA molecule at the 25th-2896th site of which the nucleotide sequence is SEQ ID No: 1; b) a DNA fragment which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of identity with the nucleotide sequence limited by the a), is derived from alfalfa and has a promoter function; c) a DNA fragment which is hybridized with the nucleotide sequence limited by a) or b) under strict conditions and has the promoter function. The promoter can be used for improving the specific expression of genes in seeds and is applied to specific marking of plant seeds or improvement of seed traits.

The invention provides an alfalfa fungicide coating agent and application thereof. The alfalfa fungicide coating agent is prepared from 4-8 parts of an adhesive, 4-8 parts of a water-retaining agent, 20-30 parts of an arbuscular mycorrhizal fungicide and 100-300 parts of water, each 5g of alfalfa seeds are coated with 0.04 to 0.08 g of an adhesive, 0.04 to 0.08 g of a water-retaining agent, 0.2 to 0.3 g of an arbuscular mycorrhizal inoculant and 1 to 3ml of water; according to the coated alfalfa seeds prepared through the method, the emergence rate on the twelfth day can reach 81.67%, the plant height after 45 days can reach 20.08 cm, the drought resistance of alfalfa can be improved, and the root development of alfalfa is promoted.

The invention relates to the technical field of fertilizers, discloses a microbial agent synergistic type organic-inorganic compound fertilizer special for saline-alkali tolerant alfalfa and a preparation method of the microbial agent synergistic type organic-inorganic compound fertilizer, and aims at solving the problems that germination of alfalfa seeds in saline-alkali soil is blocked, the emergence rate is low and the utilization rate of soil nutrients is insufficient. The organic-inorganic compound fertilizer comprises inorganic components, organic components, compound medium trace elements and compound bacterial powder, the seed dressing microbial inoculum is prepared from paenibacillus polymyxa, sphingomonas and bacillus cereus according to a proportion, and during preparation, the granular organic-inorganic compound fertilizer is prepared through batching, granulation, drying and coating; according to the method, the synergistic effect of the fungicide and the fertilizer is achieved, the emergence rate and the seedling protection rate of alfalfa are increased, plant growth is promoted, meanwhile, the soil structure of the saline-alkali soil is improved, nutrients are activated, the salt resistance and stress resistance of alfalfa are enhanced, and planting benefits are guaranteed.

The invention belongs to the technical field of saline-alkali soil planting, and particularly relates to an efficient alfalfa planting method for avoiding salt and waterlogging in severe saline-alkali soil, which comprises the following steps: S1, from April to May, ridging in a field to build a water storage area, laying a plastic film in the water storage area to cover the whole water storage area, and uniformly punching the plastic film to form air holes; s2, from the end of August to the beginning of September, uncovering the mulching film, punching the soil to form planting holes, sowing alfalfa seeds in the planting holes, and then covering the alfalfa seeds with wet sand to form an isolation layer. According to the efficient alfalfa planting method for avoiding salt and waterlogging in the severe saline-alkali soil, rainwater is accumulated in summer to leach and reduce soil salinity, a suitable germination environment is created in combination with wet sand covering, the alfalfa emergence rate is remarkably increased, and the purpose of full seedling is achieved.

The present application belongs to the field of grass planting, and particularly relates to a microbial coating agent for relieving salt and alkali stress of alfalfa, a preparation method and application thereof. The microbial coating agent comprises endomycorrhizal fungus powder, Trichoderma harzianum powder, talcum powder, diatomite and carboxymethyl cellulose sodium aqueous solution. The endomycorrhizal fungus and Trichoderma harzianum are combined to coat alfalfa seeds, with Trichoderma harzianum as the main component and endomycorrhizal fungus as the auxiliary component. A small amount of endomycorrhizal fungus and Trichoderma harzianum are used to coat alfalfa seeds, which can relieve salt and alkali stress, significantly improve the emergence rate, promote the increase of alfalfa plant height, root length and biomass, promote the growth of alfalfa, and improve the stress resistance. The microbial coating agent has few components, low fungus content and low cost, and is suitable for large-scale popularization and use.

The application provides application and methods of spermine in promoting germination and seedling growth of plants under drought conditions, and relates to the technical field of plant cultivation. The application of spermine and the method thereof provided by the application are simple in operation, can quickly and effectively promote the germination of alfalfa seeds and the growth of seedlings under drought stress, and are beneficial to popularization in the planting and production of alfalfa in arid areas.

The invention discloses an agrobacterium strain EC17 for improving salt tolerance of alfalfa and application of the agrobacterium strain EC17. Wherein the agrobacterium strain EC17 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33194. The agrobacterium strain EC17 for improving salt tolerance of alfalfa has the functions of dissolving organic phosphorus and inorganic phosphorus, dissolving potassium, producing a homogeneous membrane, producing IAA and producing ACC deaminase. Under the stress of 200mmol / L and 250mmol / L NaCl, the germination index of the alfalfa seeds can be obviously improved. Under the stress of 200mmol / L NaCl, the growth index of alfalfa seedlings can be obviously improved.

The invention relates to a planting method for improving the yield and quality of alfalfa, and relates to the technical field of alfalfa seedling cultivation, the planting method comprises the steps of land selection and preparation, seed treatment, sowing, field management and harvesting and stubble reserving; the alfalfa seed pretreatment step comprises the following steps: soaking alfalfa seeds in an aqueous solution containing a chionanthus retusus leaf extract, and then fishing out and drying the alfalfa seeds. According to the method disclosed by the invention, the chionanthus retusus leaf extract is adopted to soak and pretreat the alfalfa seeds, so that the yield of the alfalfa can be remarkably increased, the crude protein content in the alfalfa can be increased, the quality of the alfalfa is improved, and the use requirements of animal husbandry are met.

The invention belongs to the technical field of efficient pod breaking and collecting of alfalfa seeds, particularly relates to an efficient pod breaking and collecting device and method for the alfalfa seeds, and aims to solve the problems that in the prior art, when the alfalfa seeds are harvested, hulled and discharged, due to the fact that materials are accumulated, blockage is prone to occurring, the materials are dried unevenly after harvesting, and the working efficiency is reduced. According to the technical scheme, the device comprises a box body, one side of the box body is rotationally connected with a driving rotating rod, the driving rotating rod is fixedly connected with a driving gear, the interior of the box body is rotationally connected with a first rotating rod and a second rotating rod, and one end of the first rotating rod and one end of the second rotating rod are both fixedly connected with driven gears; and the two driven gears are meshed with the driving gear. The device is simple in structure, during shelling and discharging, the discharging pipe can be knocked in a reciprocating mode, the occurrence situation is avoided, the device can be evenly dried, the working efficiency is improved, and people can conveniently use the device.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.