Alfalfa seed soaking liquid and related method

Abstract

The invention provides an alfalfa seed soaking liquid and a related method. The alfalfa seed soaking liquid comprises 2, 4-epi-brassinosterold, KNO3, ethyl alcohol and water, wherein dosage ratio of 2, 4-epi-brassinosterold to KNO3, to ethyl alcohol to the water is (0.01-1.0mg): 20g: 0.5ml: 1L. The method comprises the step of before sowing, soaking the alfalfa seeds by the alfalfa seed soaking liquid. The method is simple in process, low in cost and good in effect, and the problem that the germination rate of the alfalfa seeds is low under the high salt stress in the production of alfalfa can be effectively solved.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a solution for soaking alfalfa seeds and a method for using the solution to promote germination of alfalfa seeds under high-salt stress. Background technique [0002] Alfalfa (Medicago sativa L.) is an important leguminous forage, known as the "king of forage" because of its high nutritional value and good palatability. At present, my country has more than 20 million hectares of saline-alkali land and more than 0.07 billion hectares of saline-alkali soil, accounting for about 20% of the cultivated land area. The development and utilization of saline-alkali land and the development of alfalfa production will improve the utilization efficiency of agricultural land resources, expand the alfalfa planting area, and improve high-quality forage. Grass protection ability is of great significance. However, under saline-alkali stress, especially high-salt stress, the germinat...

AI Technical Summary

Problems solved by technology

Yet this method has certain defects: 1) it can only improve the germination rate of alfalfa seeds under the condition of low salt stress (salt content is lower than 0.6%), and the effect is not obvious under the stress of medium and high concentration salt concentration, and can not solve the high salt stress Problems with germination of alfalfa seeds; 2) The technical process of this method is complicated and has high requirements on the environment. During the dehydration process of seeds, silica gel is needed. Gradually restore water under gradient, the operation process is complicated and the cost is high

However, this method has poor water retention capacity of the sand, which makes it difficult to control the water content during the treatment process, resulting in unstable treatment effects. In addition, it is difficult to separate the treated seeds from the sand, making it difficult to be widely used.

[0006] 3) PEG and 2% KNO 3 -KH 2 PO 3 Initiation treatment: Lou Jianfeng (Lou Jianfeng, Xie Xiujuan, Hu Jin, Qiu Jun, The effect of different initiation treatments on the germination of alfalfa seeds and the physiological and biochemical changes of seedlings under salt stress) found that 20% PEG6000U, 2% KNO 3 , 2% KNO 3 -KH 2 PO 3 and other treatments can improve the germination ability of alfalfa seeds under salt stress to varying degrees, but these methods can only promote the germination of alfalfa seeds under moderate salt stress (salt content <0.8%), and the effect is not very good under high salt stress. Well, the same doesn't solve the problem of alfalfa seed germination at high salt concentrations

Moreover, the concentration of PEG is high, the price is expensive, and the processing cost in actual production is high, so it is difficult to be widely used.

[0007] In summary, although there are some technical solutions that can partially solve the problem of promoting the germination of alfalfa seeds under low-salt stress, there are problems such as complicated operation procedures, high treatment costs, poor effects, high environmental requirements, and difficulties in subsequent seed treatment. Importantly, none of them can effectively solve the key technical problems of alfalfa seed germination under high-salt stress

Some current technical schemes only partially solve the germination problem of alfalfa seeds under moderate salt stress (soil salt content is lower than 0.8%), and alfalfa seed germination and seedling growth under high-intensity salt stress (salt content> 1.0%) problem still to be solved