107results about How to "Solve the low germination rate" patented technology

Providing a method for improving the germination of plant seeds for use in agriculture, gardening and tree planting on mountains and in forests, where the germination of the seeds is suppressed physically or physiologically due to the hard seed coat (testa or pericarp or the like) as the structures surrounding seed embryos, by secure and uniform treatment of each seed without any damage of seed embryo to produce seeds with improved germination in a stable manner without any loss and without creating problems in the working environment, and providing germination improved seeds. Germination is improved by allowing laser beam to irradiate a part of the seed coat of a plant seed to perforate a part of the seed coat to thereby overcome physical or physiological factors causing the suppression of germination, such as gas permeability and water permeability to seed embryo.

The invention discloses a tissue culture method of dendrobium candidum. The method comprises the following steps of: firstly culturing in dark in a seeding culture medium for a week and then transferring to be under light to culture; after performing aseptic germination for 25-35 days, transferring in a protocorm multiplication culture medium to perform multiplication culture for 25-35 days; and then sequentially performing differentiation culture in a protocorm differentiation culture medium for 40-50 days, subculture in a subculture medium for 55-65 days, strong seedling culture in a strong seedling culture medium for 55-65 days and rooting culture in a rooting culture medium for 40-50 days. A tissue culture rapid propagation method of the dendrobium candidum can be free from seasonal constraint and can realize industrialized production all the year, the environment is easy to control, and the aseptic germination rate of dendrobium candidum seeds reaches up to over 96.8 percent; and according to the method, the multiplication multiple is increased, a large number of high-grade seedlings can be rapidly bred, a precursor substance is added, and the dendrobium polysaccharide content of a dendrobium candidum test-tube plantlet is effectively accumulated.

The invention discloses a treatment agent for hard seeds, relates to a seed treatment agent, and belongs to the technical field of agriculture. The invntion is characterized by comprising low temperature treatment, normal temperature treatment, high temperature treatment and microbial agent treatment. The invention has the following beneficial effects: temperature is employed to conduct stimulation treatment on the seeds, and the stimulation treatment comprises steps of: low temperature treatment, normal temperature treatment, high temperature treatment and microbial agent treatment. The microbial agent contains Bacillus subtilis and thiamethoxam. Poikilothermic treatment on seeds has advantages of improving the seed germination rate, sterilizing, reducing crop diseases and pests at seedling period and reducing the use of pesticides. Microbial agent treatment breaks confinement of traditional physical and chemical methods for seed treatment. The invention provides a simple and feasible biological treatment method from a unique perspective and rational microbial agent combination to solve the long-standing problem of low seed germination rate, and has great significance to the green environmental protection; and the method provided by the invention has no toxic effects on germinating seeds, and can benefit germination due to temperature stimulation and nutrients from the microbial agent.

The invention relates to a culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen. The culture medium is prepared by adding gibberellin, naphthylacetic acid, indoleacetic acid or 2,4-dichlorphenoxyacetic acid serving as a growth regulator into agar, sucrose and boric acid which serve as basic culture medium components, or adding potassium nitrate, magnesium sulfate, urea, calcium sulfate, ammonium molybdate, sodium molybdate, ferric sulfate, zinc sulfate or copper sulfate serving as a chemical element into agar, sucrose and boric acid which serve as basic culture medium components in a mass ratio. The culture medium solves the problems of low germination rate of the pollen and slow growth of pollen tubes, and an effective path for improving the germination rate is found for Kumaiti. Through the culture medium, the germination and the growth of Luntai Armeniaca vulgaris Lam pollen are improved; and the culture medium is easy and convenient to operate, low in cost, short in period and high in germination rate, and has wide application prospect in drupe tree biology.

The invention discloses a tissue culture method for cymbidium tortisepalum and application thereof. The method comprises the steps of taking cymbidium tortisepalum aseptic seedling stem tip as an explant, taking a proliferation medium to induce rhizomes to proliferate, taking an LED (light-emitting diode) as a tissue culture light source, and culturing by using blue light; then transferring the obtained rhizomes to a subculture growth medium, inducing multiple shoots to grow and a seedling to quickly develop, taking the LED as the tissue culture light source, and culturing by using red light; inoculating the newly generated seedling to a rooting medium for strong seedling rooting culture, taking the LED as the tissue culture light source, and culturing by using mixed red light and blue light which are matched according to a ratio of (2 to 4):(6 to 8). By adopting the tissue culture method, the problems of low sprouting ratio, slow growth, difficult rooting and the like existing in the prior tissue culture technology are effectively solved; by using LED lamps as the tissue culture light source, 30 to 50 percent of electricity resource is saved, the cost is saved, and the efficiency of industrialized production is improved.

The invention belongs to the field of agricultural waste cyclic utilization, in particular to a degradable seedling culturing bowl prepared with straw and a preparation method thereof. The degradable seedling culturing bowl prepared with the straw is prepared with, by weight, 50-100 parts of sweet potato straw, 50-100 parts of wheat straw, 50-100 parts of soybean straw, 50-100 parts of cotton straw, 5-10 parts of astragalus membranaceus herb residues, 1-5 parts of isatis root residues and 0.2-2 parts of addition agents. The seedling culturing bowl is good in water storage performance and medium in blow wall strength, unshelling is not needed after seedlings culturing is completed, the bowl is not harmful to roots and seedlings, the seedlings in the bowl are convenient to transport, integrated transplantation is facilitated, the bowl can decompose in soil naturally, no pollution is caused to the soil, and a certain fertilizing effect is achieved.

The invention discloses a high-yield planting method for Hongjinlong mangoes. The method comprises the steps of carrying out site selection and land preparation, carrying out seed treatment, carrying out field planting, carrying out nutritional-agent dressing, and the like, wherein in a nutritional-agent dressing process, nutritional-agent dressing is carried out after growth of spring-cycle shoots, 2 weeks after blossoming, at a fruit swelling stage and a fruit strengthening stage; and nutritional-agent dressing is carried out specifically as follows: (1) after the growth of the spring-cycle shoots, a nutritional agent a is dressed once every 10 to 15 days and is dressed twice; (2) 2 weeks after blossoming, the nutritional agent a is dressed once every 7 to 10 days and is dressed for three times; (3) at the fruit swelling stage, a nutritional agent b is dressed once every 3 to 5 days and is dressed twice; and (4) at the fruit strengthening stage, the nutritional agent b is dressed once. According to the method, the yield per mu of the mangoes can be increased greatly, and the quality is better.

The present invention discloses a tomato plant exhibiting a branching inflorescence phenotype. The aforementioned plant produces statistically significant high yield of mature fruits having the characteristics of average individual fruit weight or average individual fruit brix levels or a combination thereof, similar to fruits produced by a tomato line that is used as the normally branched parent line of said tomato plant. It is also within the scope of the present invention to disclose methods for producing the aforementioned tomato plants and uses thereof.

The invention discloses a method for promoting germination of Tinospora capillipes Gagnep seeds. The Tinospora capillipes Gagnep seeds are soaked in a seed soaking chemical for 12-36 h before germination culture of the Tinospora capillipes Gagnep seeds, wherein concentrations of kinetin and gibberellin in the seed soaking chemical are 5-30 mg/L and 150-350 mg/L respectively. According to the method for promoting germination of the Tinospora capillipes Gagnep seeds, the seeds are treated with the seed soaking chemical, so that germination rate of the Tinospora capillipes Gagnep seeds is increased, germination time is shortened, germination uniformity of the seeds is improved, and Tinospora capillipes Gagnep seedings with high quality are obtained.

The present invention provides pepper plants having edible fruit with altered vitamin content, specifically fruit comprising an elevated content of vitamin E, further comprising at least one of an elevated content of pro-vitamin A, an elevated content of vitamin C and combinations thereof. The concentration of vitamin E is at least 5 mg/100 g fresh weight, and the plants are selected to be lacking molecular markers linked to undesirable traits.

The invention relates to a grape cultivation method, specially relates to a cultivation method helpful for anti-freezing of outdoor grape, which is characterized in that the cultivation method includes steps of: a. unearthing overwintering grape vine cultivated at April in spring; b. clearing up soil overwintering and cold-proofing between rows and plants of grape, winding the grape vine upon a first lead wire 0.5 meters over the ground; c. spraying stone sulfur mixture on the whole row of grape vines after the winding; d. laying plastic films at narrow row intervals; e. covering the canopy film onto a support of a second lead wire pulled on the upper portion of the pergola along the row with a height of 1 meter, and covering a sun-shading net onto the pergola. The method in very unique, capable of implementing temperature increasing and stabilizing, wind prevention and late frosting prevention.

The invention provides a tomato planting method, and relates to the technical field of vegetable planting. The tomato planting method includes steps of (1), soil selection; (2), variety selection; (3), seed drying; (4), seed immersion by medicament; (5), germination treatment; (6), soil preparation; (7), sowing; (8), seedling management; (9), fixed planting; (10) field management; (11) harvesting. The tomato planting method solves the problems of high morbidity, low output and poor quality of the tomato planted by the existing tomato planting method.

The invention discloses a method for screening cistanche seeds. The invention discloses a method for collecting seeds of Cistanche deserticola, which comprises the following steps: collecting seeds with a diameter of 0.6-1.0 mm from seeds in the middle of the inflorescence and/or in the lower part of the mature inflorescence of the cistanche deserticola. The invention effectively solves the problems of poor seed quality and low germination rate in the large-scale planting process of Cistanche deserticola.

The invention discloses a seed introduction method of submerged plant vallisneria, which comprises the steps of collecting ripening fruits of the vallisneria, removing epicarp, obtaining seeds, washing the seeds with clean water within 0-24h to keep the seeds moist, transporting the collected seeds to a destination by a water-culture method, mixing the seeds transported to the destination with moist river sand, refrigerating the seeds at 0-10 DEG C, preserving the seeds by shading above 95% till complete darkness, ventilating and replenishing water frequently, maintaining a moisture content of each seed to be 35-50%, sowing the preserved seeds in a mixed matrix, a soil matrix or a yellow clay matrix, controlling a temperature to be 10-30 DEG C, the illumination intensity to be 800-3500LUX, and the everyday illumination time to be 10-12h, allowing the seeds to germinate and grow, obtaining germinated seedlings, planting the germinated seedlings in the mixed matrix with a 10-15cm water level for culturing, managing other conditions according to a convention, planting the vallisneria, and allowing the vallisneria to grow. The mixed matrix comprises the following components by total mass fraction: 20-40% of sand and 60-80% of soil. The seed introduction method is long in seed preservation time, high in germination rate, and high in emergence rate and planting survival rate.

The invention discloses a culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation. The culture medium box set comprises a culture medium 1 for primary culture, a culture medium 2 for secondary culture, a culture medium 3 for toughening seedlings and a culture medium 4 for taking roots, wherein the culture medium 1 for primary culture takes an MS (murashige and skoog) culture medium as a basal culture medium, 0.5-2mg/L of BA (butyl acrylate), 0.1mg/L of NAA (naphthalene acetic acid), 0-0.1mg/L of TDZ (thidiazuron), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 2 for secondary culture takes an MS culture medium as a basal culture medium, 0.5mg/L of BA, 0-0.1mg/L of NAA, 0-0.5mg/L of GA3 (gibberellin), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 3 for toughening seedlings takes an MS culture medium as a basal culture medium, 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 4 for taking roots takes 1/2 MS culture medium as a basal culture medium, and 0.5-1.5 mg/L of IBA (indole-butyric acid), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium. The invention further discloses a method for facilitating crgotvaunilocularis in-vitro rapid propagation by the culture medium box set. Crgotvaunilocularis is cultured by the method under optimized conditions, and ideal reproduction rate, rooting rate and transplanting survival rate can be achieved.

The invention provides a rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb, and relates to the field of plant tissue and cell engineering. The method specifically comprises the following steps: selecting the top end of a tender stem of newly germinated pandanus amaryllifolius Roxb, only reserving a leaf sheath attached to a bud point part as an explant, and disinfecting and sterilizing the explant; cutting off the base part of the tender stem, cutting the top end until a terminal bud is exposed, cutting the tender stem into stem sections with buds, and putting the stem sections into a callus culture medium; and after axillary buds germinate and granular calluses grow out from cuts, putting the axillary buds into an adventitious bud induction culture medium, when the axillary buds form new adventitious buds, further propagating the calluses, growing green bud points on the surfaces and developing the green bud points into tender buds, then after propagating in an adventitious bud propagation culture medium, transferring the tender buds into an adventitious bud elongation culture medium, enabling the adventitious buds to be elongated to 1.5 cm or above, sequentially placing the adventitious buds into two kinds of adventitious bud rooting culture media with a two-step rooting method, and finally, growing 5-10 roots out from the adventitious buds. According to the technical scheme, the rapid propagation method of the tissue culture seedlings of the pandanus amaryllifolius Roxb is enriched, and the induction rate of the adventitious buds and the rooting rate of the seedlings are increased.

The invention belongs to the technical field of reproduction of plant seedlings and relates to a method for increasing germination speed of arenga engleri seeds. The method comprises the following steps of: immediately removing seed husks of newly harvested, mature and plump arenga engleri seeds, and then putting into a squeezed arenga engleri seed husk juice to be soaked for 6-8 days; taking out the seeds soaked in the arenga engleri seed husk juice and then soaking in clean water till germination occurs. According to the method for increasing germination speed of arenga engleri seeds, the arenga engleri seed husk juice is adopted for soaking the arenga engleri seeds, so that the germination of the seeds is promoted and problems of long germination time and low germination rate of arenga engleri seeds in the prior art can be solved effectively; and the method for increasing germination speed of arenga engleri seeds has the advantages of simple process, low cost, short germination time and uniform germination and the like, is beneficial to large-scale production of arenga engleri seedlings and provides an effective manner for the popularization and application of arenga engleri seedlings in landscape greening.

A plant seed harvesting device comprises a multi-layer net unit; the multi-layer net unit comprises collecting net, intermediate layer net and bottom layer net; the collecting net, the intermediate layer net and the bottom layer net are all plate-like structure having a plurality of meshes; the peripheral edge of the plate-like structure is a net frame, area with the meshes is net face; The collecting net, the intermediate layer net and the bottom layer net are arranged closely in the axial direction to form the multi-layer net unit; mesh sizes on the collecting net, the intermediate layer net and the bottom layer net are configured to be successively and gradually decreased; plant seeds can pass through the meshes of the collecting net; air but not plant seeds can pass through the meshes of the bottom layer net.