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Tissue culture method of dendrobium candidum

A technology of tissue culture and dendrobium candidum, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of unsatisfactory natural yield, depletion of wild resources, scarcity of natural resources, etc. Market Prospects, Effects of Excellent Properties

Inactive Publication Date: 2013-03-06
贵州易通实业股份有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, because wild Dendrobium officinale mainly grows on the cliffs of high mountains, because its seeds are extremely small, each capsule contains about 2,000 seeds, which are as fine as dust and have no endosperm. The germination rate is extremely low under natural conditions, and natural resources are very scarce.
Coupled with years of unrestrained mining, wild resources are becoming increasingly depleted, and the natural output cannot meet the current market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the tissue culture method of Dendrobium candidum, comprises the following steps:

[0024] (1) Sowing: Select full capsules aged 4-6 months, disinfect the surface with 75% alcohol, and sprinkle the seeds evenly on the sowing medium. The formula of the sowing medium is: 1 / 2MS+white sugar 2%+potato juice 15%, cultured in the dark for one week, then transferred to the light culture, after 25-35 days of aseptic germination, it can be transferred to the protocorm proliferation medium, and the seed germination rate can reach 96.30%;

[0025] (2), protocorm proliferation and differentiation:

[0026] Insert the germinated seeds of step (1) into the protocorm proliferation medium for proliferation and culture for 25 to 35 days. The formula of the protocorm proliferation medium is: 1 / 2MS + naphthaleneacetic acid (0.2-0.5mg / L) + white sugar 2% + 10% potato juice, and then insert the seeds into the protocorm differentiation medium for differentiation and culture for ...

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Abstract

The invention discloses a tissue culture method of dendrobium candidum. The method comprises the following steps of: firstly culturing in dark in a seeding culture medium for a week and then transferring to be under light to culture; after performing aseptic germination for 25-35 days, transferring in a protocorm multiplication culture medium to perform multiplication culture for 25-35 days; and then sequentially performing differentiation culture in a protocorm differentiation culture medium for 40-50 days, subculture in a subculture medium for 55-65 days, strong seedling culture in a strong seedling culture medium for 55-65 days and rooting culture in a rooting culture medium for 40-50 days. A tissue culture rapid propagation method of the dendrobium candidum can be free from seasonal constraint and can realize industrialized production all the year, the environment is easy to control, and the aseptic germination rate of dendrobium candidum seeds reaches up to over 96.8 percent; and according to the method, the multiplication multiple is increased, a large number of high-grade seedlings can be rapidly bred, a precursor substance is added, and the dendrobium polysaccharide content of a dendrobium candidum test-tube plantlet is effectively accumulated.

Description

technical field [0001] The invention relates to a cultivation method technology of dendrobium candidum, in particular to a tissue culture method of dendrobium candidum. Background technique [0002] Dendrobium officinale is a famous species of Dendrobium with unique medicinal value. The "Shen Nong's Materia Medica" in the Qin and Han Dynasties records that Dendrobium candidum "mainly injures, eliminates numbness, lowers qi, nourishes the five viscera, strengthens yin, and thickens the stomach after long-term use"; modern chemical and pharmacological studies have shown that the main effects of Dendrobium candidum The ingredients are polysaccharides, and the polysaccharide content is as high as 20% to 30%. Because of its polysaccharides, alkaloids, amino acids, trace elements and other ingredients contained in Dendrobium can enhance the body's immune ability, anti-oxidation, anti-aging, lower blood sugar, nourish yin and promote body fluid, strengthen kidney and essence, inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张建民朱新明
Owner 贵州易通实业股份有限公司
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