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Tissue culture method of morus mongolica seedlings

A technology for tissue culture and seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that there is no tissue culture method for Monsang seedlings, and achieve the effect of shortening the breeding cycle and increasing the breeding coefficient

Active Publication Date: 2019-06-11
INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no corresponding tissue culture method for Monsang seedlings

Method used

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  • Tissue culture method of morus mongolica seedlings
  • Tissue culture method of morus mongolica seedlings
  • Tissue culture method of morus mongolica seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0056] (1) After the dormant Mengsang branches are gently scrubbed with 0.5% washing powder water with a mass concentration, they are rinsed with tap water until there is no foam. In the ultra-clean workbench, soak the branches with dormant buds in alcohol with a volume fraction of 75% for 40 seconds, then wash them with sterile water for 3 to 5 times, and then sterilize them with a 0.1% mercury chloride solution for 15 minutes. Rinse 5 times with sterile water.

[0057] (2) The shoots sterilized in the step (1) are stripped with a scalpel to shoot shoot tips with a length of 0.3-0.5 cm, and inoculated in the induction medium. The induction medium is based on MS medium, and is obtained by adding 6-BA, IBA, PVP-40, sucrose and agar. The concentration of 6-BA in the induction medium is 1.0mg / L, and the concentration of IBA is 0.5mg / L. L, the concentration of PVP-40 is 500mg / L, the concentration of sucrose is 20g / L, the concentration of agar is 5.5g / L, and the pH value is 5.8~6....

Embodiment 1-2 to Embodiment 1-5

[0063] Change the type and amount of reagents in the induction medium in step (2), and observe the effects of different hormone combinations on the germination rate and growth of Monsang. The dosage of sucrose and agar, and the cultivation method are the same as those in Example 1-1, and the results are shown in Table 1.

[0064] Table 1

[0065]

[0066] As can be seen from Table 1, compared with Example 1-1, the average plant height and growth of Monsang induced seedlings were all decreased by changing the added concentration of 6-BA, IBA and PVP-40. Therefore, for the induction culture step, the most suitable induction medium formula is MS medium + 1.0mg / L 6-BA + 0.5mg / L IBA + 500mg / L PVP-40.

Embodiment 1-6 to Embodiment 1-9

[0068] Change the type and amount of reagents in the subculture medium in step (3), and observe the effects of different hormone combinations on the growth of Monsang. The dosage of sucrose and agar, and the culture method are the same as in Example 1-1, and the results are shown in Table 2. The results were calculated using the method of averaging.

[0069] Table 2

[0070]

[0071] As can be seen from Table 2, compared with embodiment 1-1, change IBA and GA 3 The average plant height and number of stem nodes of Mengsang secondary seedlings decreased. Therefore, for the subculture step, the most suitable subculture medium formula is 1 / 2MS medium + 0.5mg / L IBA + 3mg / L GA 3 .

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Abstract

The invention provides a tissue culture method of morus mongolica seedlings. The method comprises the following steps of taking morus mongolica dormant buds as explant materials and MS as a basic culture medium and adding 6-BA, 2,4-D, IBA, GA3, PVP-40 and other substances with different concentrations into the culture medium; sequentially carrying out subculture, proliferation culture and rootingculture on the morus mongolica explants to obtain complete plants. By adopting the method, a large number of regenerated morus mongolica seedlings can be obtained, and the problems of variation and slow growth caused by seed propagation and spread of plant diseases and insect pests resulted from a cutting mode are solved. The method can obviously increase the morus mongolica breeding coefficient and shorten the breeding cycle. According to the method, proliferation is carried out at the speed rate of 9.3 times in the proliferation period, and the rooting rate is 100%.

Description

technical field [0001] The invention relates to the technical field of tree breeding, in particular to a method for tissue culture of mongomulus seedlings. Background technique [0002] Morus mongolica, also known as rock mulberry, is an ideal ecological and economical tree species, which can play a very active role in soil and water conservation and ecological restoration in northern regions. It is mainly distributed in the Daqing Mountains, the loess hills in the south of the Yinshan Mountains, the mountains in the south of the Daxing'an Mountains, and the Daqinggou Nature Reserve in Kezuohou Banner. [0003] Monsang is a cross-pollinated plant, and the seedlings propagated by sowing tend to mutate, and cannot maintain the excellent traits of the female parent, and the seedlings grow slowly at the seedling stage, with long seedling breeding cycles and low reproductive coefficients. Although cutting propagation can maintain the excellent traits of the female parent, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赵泉胜铁英白玉娥彭鹏邰海欧李红颖
Owner INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD
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