Compound bacteria preparation and preparation method and application thereof

A technology of complex bacteria and preparations, applied in biochemical equipment and methods, chemical instruments and methods, methods based on microorganisms, etc., can solve the problems of water environment ecosystem imbalance, affecting the healthy growth of aquatic animals, and increasing polluted organic matter. Achieve the effects of promoting healthy breeding and efficient production, enhancing stability and reducing production costs

Active Publication Date: 2018-03-23
GUANGZHOU JINSHUI ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In high-density intensive aquaculture water, a large amount of residual bait and excrement of aquatic animals have accumulated, resulting in an increase in polluted organic matter, loss of balance in the water environment ecosystem, and serious excess of nitrite in the water, causing a large area of ​​aquatic animal diseases The outbreak affected the healthy growth of aquatic animals

Method used

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  • Compound bacteria preparation and preparation method and application thereof
  • Compound bacteria preparation and preparation method and application thereof
  • Compound bacteria preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take the slant of Bacillus coagulans, pick 2 rings of lawn with an inoculation loop, inoculate it in nutrient broth, and cultivate it on a shaking table at 37°C for 16 hours to obtain the activated bacterial liquid; take the seeds of Clostridium butyricum glycerol tube, Inoculate in RCM medium and culture at 37°C for 24 hours to obtain activated bacterial liquid; take one slant of Lactobacillus acidophilus and one of Candida utilis slant, and pick 2 rings of Lactobacillus acidophilus with an inoculation loop Moss, inoculated in YPD liquid medium, and then pick Candida utilis moss with an inoculation loop, inoculate it in the same YPD liquid medium, and culture it statically at 28°C for 3 days to obtain a mixture of lactic acid bacteria and yeast seed liquid. (2) Preparation of fermentation medium: glucose 10g / L, yeast powder 3g / L, sodium chloride 1g / L, soybean meal 16g / L, corn steep liquor 5g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 0.5 g / L, mangan...

Embodiment 2

[0025] Determination of nitrite degradation in simulated pond water.

[0026] Take nine 1L sterile screw-top bottles, fill about 1L of culture pond water, add shrimp feed 2g / L, ammonium chloride 0.042g / L, sodium nitrite 0.025g / L, and sterilize at 115°C for 30 minutes. Group 1 was inoculated with 1% Lactobacillus acidophilus, Group 2 was inoculated with 1% Candida utilis, Group 3 was inoculated with 1% Clostridium butyricum, Group 4 was inoculated with 0.33% compound bacteria of Example 1, and Group 5 was not inoculated with control bacteria. 30°C, in a constant temperature incubator, let it stand still, and regularly measure the content of nitrite, and the nitrite is determined by N-(1-naphthyl)-ethylenediamine spectrophotometry. The test results are shown in Table 1.

[0027] Table 1 The nitrite degradation rate of each strain in the simulated pond water

[0028]

[0029] The above test results show that the composite bacteria of the present invention have a stronger abi...

Embodiment 3

[0031] Select 6 test buckets, pour into 100 liters / barrel of culture water, water depth 1.0 meters, put 30 tails of shrimp seedlings in every bucket. The test group was put into the above-mentioned compound bacteria preparation, the dosage was 0.30 ml / barrel, and the test pool of the control group was not put into the compound bacteria preparation, and the NO was detected synchronously every two days. 2 - -N. Observing the growth of the prawns for 8 consecutive days. Nitrite was determined using a nitrite rapid detection kit produced by Guangzhou Liyang Aquatic Technology Co., Ltd. The test results are shown in Table 2.

[0032] Table 2 Changes of nitrite in water body with time

[0033]

[0034]

[0035] Test results: the above test results showed that the nitrite content of the test group was reduced by more than 60% on the 8th day in the embodiment of the present invention, and the prawns grew well, fully illustrating that the composite bacteria preparation of the ...

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Abstract

The invention belongs to the field of aquaculture and discloses a compound bacteria preparation and a preparation method and application thereof. The preparation method includes: (1), preparing seeds;(2), inoculating bacillus coagulans; (3), inoculating clostridium butyricum; (4), inoculating lactobacillus and saccaromycetes mixed seed liquid to obtain the compound bacteria preparation. The compound bacteria preparation can be applied to aquaculture and can effectively degrade nitrite in an aquaculture pond, purify water and promote healthy culture and efficient production of aquaculture industry of China.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a composite bacteria preparation and its preparation method and application. Background technique [0002] In high-density intensive aquaculture water, a large amount of residual bait and excrement of aquatic animals have accumulated, resulting in an increase in polluted organic matter, loss of balance in the water environment ecosystem, and serious excess of nitrite in the water, causing a large area of ​​aquatic animal diseases The outbreak affected the healthy growth of aquatic animals. Microorganisms can restore water bodies, and have the characteristics of non-toxicity, no side effects, no residue, no pollution, safety and reliability, long duration, and good water stabilization effect, and have attracted more and more attention from the aquaculture industry. [0003] Clostridium butyricum, also known as butyric acid bacteria, has long been used as a probiot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/07C12R1/145C12R1/23C12R1/72C02F101/16C02F103/20
CPCC02F3/341C02F3/347C02F2101/166C02F2103/20C12N1/16C12N1/20
Inventor 江文涛马加军贾朋辉邱勇张声峰
Owner GUANGZHOU JINSHUI ANIMAL HEALTH PROD
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