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Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen

A technology for pollen germination and culture medium, which is applied to plant cells and other directions, can solve the problems of low pollen germination rate, difficult pollination and fertilization, short pollen tubes, etc., and achieves the effects of promoting pollen germination, convenient purchase and low price.

Inactive Publication Date: 2012-08-01
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium solves the problems of low pollen germination rate, short pollen tube and difficulty in pollination and fertilization, and finds an effective way for Kumaiti to increase germination rate

Method used

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  • Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen
  • Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen
  • Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen

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Experimental program
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Effect test

Embodiment

[0041] All reagents and equipment used are well known in the art.

[0042] Selection and processing of pollen:

[0043] Select flower buds at the bell stage, peel off the anthers and store them in a sulfuric acid carton, place them in the shade at room temperature, wait for the anthers to split and release pollen, collect them into a 1.5ml centrifuge tube, seal them, and store them in the freezer of the refrigerator;

[0044] Cultivation method and time:

[0045] Medium configuration: use 1% agar, respectively put in the concentrations of: 5%, 10%, 15%, 20% or 25% of sucrose and the concentrations of: 0, 0.01%, 0.03%, 0.05% or 0.1% For boric acid treatment, invert different concentrations of medium on the glass slide, use cotton swabs to evenly pop the pollen on the medium of the slide, place it in a petri dish with wet gauze at the bottom, repeat 3 times for each gradient, Place them in an incubator at a temperature of 25°C, and observe the germination rate and the length o...

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Abstract

The invention relates to a culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen. The culture medium is prepared by adding gibberellin, naphthylacetic acid, indoleacetic acid or 2,4-dichlorphenoxyacetic acid serving as a growth regulator into agar, sucrose and boric acid which serve as basic culture medium components, or adding potassium nitrate, magnesium sulfate, urea, calcium sulfate, ammonium molybdate, sodium molybdate, ferric sulfate, zinc sulfate or copper sulfate serving as a chemical element into agar, sucrose and boric acid which serve as basic culture medium components in a mass ratio. The culture medium solves the problems of low germination rate of the pollen and slow growth of pollen tubes, and an effective path for improving the germination rate is found for Kumaiti. Through the culture medium, the germination and the growth of Luntai Armeniaca vulgaris Lam pollen are improved; and the culture medium is easy and convenient to operate, low in cost, short in period and high in germination rate, and has wide application prospect in drupe tree biology.

Description

technical field [0001] The invention relates to the technical field of pollination and fertilization of fruit trees, in particular to a culture medium for improving the germination and growth of Kumaiti pollen. Background technique [0002] Apricot (Armeniaca vulgaris Lam) is a small deciduous tree belonging to the family Rosaceae. Apricot fruit is rich in vitamin A, vitamin C, sugar, citric acid, malic acid and other nutrients. The characteristics of detoxification have certain health care and anti-cancer effects. No matter it is eaten fresh or processed, it is favored by people. Chinese apricots are widely distributed in Hebei, Beijing, Henan, Shandong, Shanxi, Shaanxi, Gansu, Xinjiang and other places, with more than 2,000 varieties. Kumaiti is one of the excellent apricot varieties in Xinjiang. It can be dried or eaten fresh. It is used as the main planting or pollination variety in Luntai, Xinjiang and other places, and has a large area of ​​planting. The fruit is ova...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 廖康孙慧瑛安晓芹
Owner XINJIANG AGRI UNIV
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