Rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb

A technology of pandan group and seedlings, applied in the field of plant tissue and cell engineering, can solve the problems of insufficiently developed root system, long time for callus induction and propagation, etc.

Active Publication Date: 2021-07-30
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +1
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical application, it is found that the base of the stem segment that is close to the root end, semi-lignified, and 3-5

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb
  • Rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb
  • Rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example

[0049] Outthlomerate collection: In the morning, the weather is sunny in a consecutive day, the mother strain growing and healthy, the pestle, collecting light yellow, new germination of Zenzhilan 4-18cm tender stem, cut away the leaf sheath of the bud part, This is an explant; the collection and preparation process of the specific exhibition figure 1 From left to right, the first 3 sets of pictures.

[0050] Exhibitors cleaning, disinfecting: Rinse the tender stems under the water, pay attention to the blade, rinse the dust in the leaf sheath, then put it into a bug containing the cleansing, gently washing the tender surface, and gently in the water Painting, rush down the surface of the stem, then transfer to the ultra-clean workbench, the effective ingredient is 0.05% geeko-leaning solution to gently shake for 10 minutes, 75% alcohol surface disinfection for 30 seconds, 0.1% chloride disinfection for 5 minutes, Rinse 3-5 times with sterile water, 2-3 minutes ea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb, and relates to the field of plant tissue and cell engineering. The method specifically comprises the following steps: selecting the top end of a tender stem of newly germinated pandanus amaryllifolius Roxb, only reserving a leaf sheath attached to a bud point part as an explant, and disinfecting and sterilizing the explant; cutting off the base part of the tender stem, cutting the top end until a terminal bud is exposed, cutting the tender stem into stem sections with buds, and putting the stem sections into a callus culture medium; and after axillary buds germinate and granular calluses grow out from cuts, putting the axillary buds into an adventitious bud induction culture medium, when the axillary buds form new adventitious buds, further propagating the calluses, growing green bud points on the surfaces and developing the green bud points into tender buds, then after propagating in an adventitious bud propagation culture medium, transferring the tender buds into an adventitious bud elongation culture medium, enabling the adventitious buds to be elongated to 1.5 cm or above, sequentially placing the adventitious buds into two kinds of adventitious bud rooting culture media with a two-step rooting method, and finally, growing 5-10 roots out from the adventitious buds. According to the technical scheme, the rapid propagation method of the tissue culture seedlings of the pandanus amaryllifolius Roxb is enriched, and the induction rate of the adventitious buds and the rooting rate of the seedlings are increased.

Description

technical field [0001] The invention belongs to the field of plant tissue and cell engineering, and in particular relates to a rapid breeding method for pandan tissue culture seedlings. The invention also provides a rapid propagation method suitable for large-scale industrial production of pandan blue seedlings. Background technique [0002] Banlan, scientific name Pandanus amaryllifolius (Roxb.), also known as pandan leaves, banlan leaves, and colorful leaves, is a perennial evergreen spice plant of the genus Pandanus in the family Pandanaceae. The place of origin is Maggulu Islands in Indonesia. It is mainly distributed in tropical and subtropical regions of the Eastern Hemisphere. It is planted in some cities and counties in Hainan, my country. There are more than 800 kinds of plants in 3 genera of Pandanaceae. Among them, Pandanus is a plant with a unique "Zongxiang" fragrance in this genus. The fragrance mainly comes from its leaves. The "brown fragrance" aroma compone...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/00
Inventor 黄天带彭素娜顾晓川黄华孙徐正伟吴日智蔡海滨廖子荣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap