Rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb

Abstract

The invention provides a rapid breeding method for tissue culture seedlings of pandanus amaryllifolius Roxb, and relates to the field of plant tissue and cell engineering. The method specifically comprises the following steps: selecting the top end of a tender stem of newly germinated pandanus amaryllifolius Roxb, only reserving a leaf sheath attached to a bud point part as an explant, and disinfecting and sterilizing the explant; cutting off the base part of the tender stem, cutting the top end until a terminal bud is exposed, cutting the tender stem into stem sections with buds, and putting the stem sections into a callus culture medium; and after axillary buds germinate and granular calluses grow out from cuts, putting the axillary buds into an adventitious bud induction culture medium, when the axillary buds form new adventitious buds, further propagating the calluses, growing green bud points on the surfaces and developing the green bud points into tender buds, then after propagating in an adventitious bud propagation culture medium, transferring the tender buds into an adventitious bud elongation culture medium, enabling the adventitious buds to be elongated to 1.5 cm or above, sequentially placing the adventitious buds into two kinds of adventitious bud rooting culture media with a two-step rooting method, and finally, growing 5-10 roots out from the adventitious buds. According to the technical scheme, the rapid propagation method of the tissue culture seedlings of the pandanus amaryllifolius Roxb is enriched, and the induction rate of the adventitious buds and the rooting rate of the seedlings are increased.

Description

technical field [0001] The invention belongs to the field of plant tissue and cell engineering, and in particular relates to a rapid breeding method for pandan tissue culture seedlings. The invention also provides a rapid propagation method suitable for large-scale industrial production of pandan blue seedlings. Background technique [0002] Banlan, scientific name Pandanus amaryllifolius (Roxb.), also known as pandan leaves, banlan leaves, and colorful leaves, is a perennial evergreen spice plant of the genus Pandanus in the family Pandanaceae. The place of origin is Maggulu Islands in Indonesia. It is mainly distributed in tropical and subtropical regions of the Eastern Hemisphere. It is planted in some cities and counties in Hainan, my country. There are more than 800 kinds of plants in 3 genera of Pandanaceae. Among them, Pandanus is a plant with a unique "Zongxiang" fragrance in this genus. The fragrance mainly comes from its leaves. The "brown fragrance" aroma compone...

AI Technical Summary

Problems solved by technology

However, in practical application, it is found that the base of the stem segment that is close to the root end, semi-lignified, and 3-5mm long has the disadvantages that it takes a long time for callus induction and propagation, and the root system is not well developed.

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