A kind of kiwifruit explant detoxification method

Abstract

The invention provides a method for detoxification of kiwifruit explants, which sequentially includes the following steps: explant selection: removing leaves of kiwifruit seedlings, intercepting 1-2 cm stem sections with buds, cleaning and sterilizing, blotting dry with sterile filter paper, peeling off the outer Implant meristem; induction culture: inoculate the explant meristem into the induction medium, first in the dark, and then in the light and dark to obtain clump buds; rooting culture: when the clump buds grow to 1-2.5 cm, with When there are 2 to 5 new leaves, transfer to the rooting medium, cultivate in light and dark, and get tissue culture seedlings; The present invention obtains robust detoxification seedlings through explant selection, induction culture, rooting culture and seedling transplanting in sequence, reduces production cost, improves seedling quality, and effectively solves the problem of kiwifruit seedlings being poisoned and contaminated in the prior art. The high toxicity rate and other problems are conducive to the large-scale promotion of the kiwifruit planting industry.

Description

A kind of kiwi fruit explant detoxification method technical field The invention belongs to the technical field of tissue culture, be specifically related to a kind of kiwifruit explant detoxification method. Background technique Kiwifruit, Actiniaceae Actinidia, native to the vine fruit tree of my country, is a kind of important economic fruit Tree. Kiwi fruit is rich in a variety of vitamins, especially vitamin C, and also contains a lot of minerals, arginine, etc., so Kiwi is also known as the king of fruits. Kiwi fruit is widely used in food and beverage processing, health care products and other forms. kind of kiwi As a perennial fruit tree, virus disease is a very serious disease. At present, there are no effective treatment measures and methods. There is great resistance to the promotion of peach cultivation. Kiwifruit is easy to cause variety degeneration with seed propagation, and some excellent characters of female parent are difficult to maintain, and...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a kind of kiwifruit explant detoxification method, through explant selection, induction culture, rooting culture and seedling hardening transplanting successively, obtain robust detoxification seedling, reduce production cost, improve the quality of seedlings, and effectively solve the problems of kiwifruit seedlings with high poisoning rate in the prior art, which is conducive to the large-scale promotion of kiwifruit planting industry

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