A tissue culture method for improving the survival rate of pearl guava
A pearl guava, tissue culture technology, applied in the field of plant tissue culture, can solve the problems of easy-to-contaminate bacteria, sensitivity to sterilizers, complicated culture methods, etc., and achieve the effect of promoting wound healing, plant growth, and induction culture
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] 1. Tissue culture steps:
[0026] Spray lime powder on the ground of the culture room with doors and windows closed 12 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into 1cm in length and 0.4cm in diameter; the explants are washed with running water for 5min, and after being pulled out, use 75% Soak in alcohol solution for 3 minutes, drain and soak in 8% plant treatment solution for 20 minutes to sterilize; inoculate the sterilized explants in the induction medium and induce culture in the incubator, the culture temperature is At 24°C, use a white LED light to illuminate, the light intensity is 500Lx, and the light time is 12h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator, and the culture temperature is At 24°C, use white LED ...
Embodiment 2
[0042] 1. Tissue culture steps:
[0043] Spray lime powder on the ground of the culture room with doors and windows closed 14 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into 2cm in length and 0.8cm in diameter; the explants are washed with running water for 8min, and after being pulled out, use 75% Soak in alcohol solution for 5min, drain and soak in 10% plant treatment solution for sterilization for 25min after draining; inoculate the sterilized explants in induction medium and induce culture in an incubator at a temperature of 26°C, light with white LED lights, the light intensity is 800Lx, and the light time is 16h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator, and the culture temperature is At 26°C, use white LED lights for il...
Embodiment 3
[0052] 1. Tissue culture steps:
[0053] Spray lime powder on the ground of the culture room with doors and windows closed 13 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into a length of 1.5cm and a diameter of 0.6cm; the explants are washed with tap water for 6 minutes, and the concentration is 75% after being pulled out. Soak in alcohol solution for 4 minutes, drain and soak in 9% plant treatment liquid for 22 minutes to sterilize; inoculate the sterilized explants in the induction medium and induce culture in the incubator, the culture temperature at 25°C, use white LED lights for illumination, the illumination intensity is 600Lx, and the illumination time is 14h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator. at 25°C, use white ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com