A kind of rapid propagation method of pandanus tissue culture seedlings

A technology of pandanus and seedlings, which is applied in the field of plant tissue and cell engineering, can solve the problems of underdeveloped root system, callus induction and propagation taking a long time, etc., and achieve the reduction of pollution rate, enrichment of rapid propagation methods, The effect of a reasonable number of roots

Active Publication Date: 2022-04-29
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Application Information

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Problems solved by technology

However, in practical application, it is found that the base of the stem segment that is close to the root end, semi-lignified, and 3-5mm long has the disadvantages that it takes a long time for callus induction and propagation, and the root system is not well developed.

Method used

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  • A kind of rapid propagation method of pandanus tissue culture seedlings
  • A kind of rapid propagation method of pandanus tissue culture seedlings
  • A kind of rapid propagation method of pandanus tissue culture seedlings

Examples

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Embodiment

[0049] Explant collection: On sunny mornings for more than 2 consecutive days, select a mother plant that grows vigorously and is free from diseases and insect pests, collect light yellow, newly germinated tender stems of 4-18cm of Cinnamon, cut off the leaf sheaths that are attached to the buds, Use this as an explant; the collection and preparation process of the specific explant is as follows: figure 1 From left to right, the first 3 groups of pictures are shown.

[0050] Cleaning and disinfection of explants: Rinse the tender stems one by one under running water, take care to remove the leaf sheaths, rinse the dust in the leaf sheaths, then put them into a beaker containing detergent, gently scrub the surface of the tender stems, and then gently wash them under running water. Scrub, wash away the foam on the surface of the stem section, and then transfer to the ultra-clean workbench, gently shake the 0.05% carbendazim solution for 10 minutes, disinfect the surface with 75%...

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Abstract

The application provides a rapid breeding method for Pandanus edulis tissue culture seedlings, which relates to the field of plant tissue and cell engineering. The specific method includes: selecting the top of the newly germinated tender stem of Pandanus, keeping only the leaf sheath close to the bud point as an explant and sterilizing it; cutting off the base of the tender stem, cutting the top until the terminal bud is exposed, cutting it into Put the stem segment with buds into the callus medium; after the axillary buds sprout and the incision grows granular callus, put them into the adventitious bud induction medium, and the axillary buds form new adventitious buds, and the callus Further multiplication, green buds grow on the surface and develop into young shoots, and then, after propagation in the adventitious bud proliferation medium, transfer to the adventitious bud elongation medium to make the adventitious buds elongate to more than 1.5cm, using a two-step rooting method , put into two kinds of adventitious bud rooting medium successively, finally the adventitious bud grows 5‑10 roots. The technical scheme of the present application enriches the rapid propagation method for the tissue culture seedlings of Pandanus edulis, and improves the induction rate of adventitious buds and the rooting rate of seedlings.

Description

technical field [0001] The invention belongs to the field of plant tissue and cell engineering, and in particular relates to a rapid breeding method for tissue culture seedlings of pandanus. The invention also provides a rapid propagation method suitable for the large-scale industrial production of the pandanus seedlings. Background technique [0002] Pandanus, scientific name Pandanus amaryllifolius (Pandanus amaryllifolius, Roxb.), also known as pandan leaves, banlan leaves, and colorful leaves, is a perennial evergreen spice plant of the genus Pandanus in the family Pandanaceae. The place of origin is Maggulu Islands in Indonesia. It is mainly distributed in tropical and subtropical regions of the Eastern Hemisphere. It is planted in some cities and counties in Hainan, my country. There are about 800 kinds of plants in 3 genera of Pandanaceae. Among them, Pandanus is a plant with a unique "Zongxiang" fragrance in this genus. The fragrance mainly comes from its leaves. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/00
Inventor 黄天带彭素娜顾晓川黄华孙徐正伟吴日智蔡海滨廖子荣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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