Tissue culture method for cymbidium tortisepalum and application thereof

A technology of tissue culture and lotus petal orchid, which is applied in the fields of application, horticultural methods, botany equipment and methods, etc., can solve the problem that there is no mature application technology in the application of lotus petal orchid tissue culture, which hinders the industrialized seedling cultivation of lotus petal orchid and Industrial development, poor root quality and other problems, to achieve the effect of saving electricity resources, promoting efficient and rapid tissue culture, and improving efficiency

Active Publication Date: 2016-02-24
广东翁山兰花研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inherent long growth cycle of orchids, low differentiation efficiency, poor root quality and other problems have always hindered the industrialized seedling cultivation and industrial development of lotus petals.
[0004] At present, there a

Method used

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  • Tissue culture method for cymbidium tortisepalum and application thereof
  • Tissue culture method for cymbidium tortisepalum and application thereof
  • Tissue culture method for cymbidium tortisepalum and application thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0027] Example 1 Optimization of the composition of the tissue culture medium of the lotus petals

[0028] 1. Proliferation medium

[0029] Take CymbidiumLianpan "Daxuesu" seeds on 1 / 2MS medium (the macronutrient and trace elements in MS basic medium are halved) after germination, aseptic seedlings grown for 14 months as explants. The clumping buds are cut into individual plants, and each plant is cut into about 2 cm sections and inoculated into a multiplication medium for multiplication and cultivation. The medium used 1 / 2MS medium + agar 7g / L as the control group, supplemented with 6-benzylpurine (6-BA) 1.5~3.5mg / L, naphthaleneacetic acid (NAA) 0.1~0.3mg / L, activated carbon 0.5~ 1g / L, sucrose 10-30g / L+agar 7g / L is the optimized group, and the specific ratio is shown in Table 1. pH=5.6~5.8.

[0030] Table 1

[0031] Medium formula

[0032] Each bottle was inoculated with 5 buds, and each medium formula was inoculated with 10 bottles. Use PHILIPS ordinary T8 white fluorescent t...

Example Embodiment

[0052] Example 2 The effect of using the best medium with LED

[0053] 1. Rhizome induction and budding differentiation culture

[0054] 1. Proliferation medium

[0055] A solid medium of 1 / 2MS+6-BA2.5mg / L+NAA0.3mg / L+active carbon 0.5g / L+sucrose 15g / L (pH=5.6~5.8)+agar 7g / L.

[0056] 2. Method of inoculation

[0057] Cut the cluster buds of the lotus petals seedlings (the sterile seedlings of the lotus petals "Daxuesu" grown for 14 months after germination) into individual plants, and each plant is cut into about 2cm sections and inoculated into the propagation medium for propagation and culture. Each bottle was inoculated with 5 buds, and each bottle was treated with 10 bottles.

[0058] 3. Cultivation conditions

[0059] The light period is 16h / d, and the light intensity is 20μmo1·m -2 ·S -1 , The ambient temperature is 24±1℃. The experiment set up 5 kinds of light sources with different light quality ratios (as shown in Table 7), the ratios are: ①100%R (red light), ②100%B (blue light...

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Abstract

The invention discloses a tissue culture method for cymbidium tortisepalum and application thereof. The method comprises the steps of taking cymbidium tortisepalum aseptic seedling stem tip as an explant, taking a proliferation medium to induce rhizomes to proliferate, taking an LED (light-emitting diode) as a tissue culture light source, and culturing by using blue light; then transferring the obtained rhizomes to a subculture growth medium, inducing multiple shoots to grow and a seedling to quickly develop, taking the LED as the tissue culture light source, and culturing by using red light; inoculating the newly generated seedling to a rooting medium for strong seedling rooting culture, taking the LED as the tissue culture light source, and culturing by using mixed red light and blue light which are matched according to a ratio of (2 to 4):(6 to 8). By adopting the tissue culture method, the problems of low sprouting ratio, slow growth, difficult rooting and the like existing in the prior tissue culture technology are effectively solved; by using LED lamps as the tissue culture light source, 30 to 50 percent of electricity resource is saved, the cost is saved, and the efficiency of industrialized production is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue rapid propagation, in particular to a tissue culture method and application of lotus petal orchid. Background technique [0002] Lotus petal orchid is a large group in China's genus Orchid, mainly produced in Yunnan. Because of its beautiful plant shape, changeable flower shape, rich flower color, and high market price, the wild resources of lotus petal orchid have been destructively excavated, resulting in endangered resources. Therefore, it is particularly important to study the tissue culture and rapid propagation technology of the lotus petal orchid. However, the inherent long growth cycle of orchids, low differentiation efficiency, and poor root quality have always hindered the industrialized seedling cultivation and industrial development of orchids. [0003] Light quality is an important factor affecting plant growth and development. In recent years, studies have shown that differen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨凤玺朱根发王真刘海林黄丹许庆全
Owner 广东翁山兰花研究有限公司
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