Culture medium for improving germination and growth of Luntai white apricot blossom pollen

A technology for pollen germination and culture medium, which is applied to plant cells and other directions, can solve the problems of low pollen germination rate, difficult pollination and fertilization, short pollen tubes, etc., and achieves the effects of promoting pollen germination, convenient purchase and low price.

Inactive Publication Date: 2012-06-27
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium solves the problems of low pollen germination rate, short pollen tube and difficult pollination and fertilization, and finds an effective way to increase germination rate for Luntai white apricot

Method used

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  • Culture medium for improving germination and growth of Luntai white apricot blossom pollen
  • Culture medium for improving germination and growth of Luntai white apricot blossom pollen
  • Culture medium for improving germination and growth of Luntai white apricot blossom pollen

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Experimental program
Comparison scheme
Effect test

Embodiment

[0033] All reagents and equipment used are well known in the art.

[0034] Selection and processing of pollen:

[0035] Select flower buds at the bell stage, peel off the anthers and store them in a sulfuric acid carton, place them in the shade at room temperature, wait for the anthers to split and release pollen, collect them into a 1.5ml centrifuge tube, seal them, and store them in the freezer of the refrigerator;

[0036] Cultivation method and time:

[0037]Medium configuration: use 1% agar, respectively put in the concentration: 0, 5%, 10%, 15%, 20% or 25% of sucrose and the concentration: 0, 0.01%, 0.03%, 0.05% or 0.1 % boric acid treatment, put different concentrations of culture medium upside down on the glass slide, use cotton swabs to evenly pop the pollen on the glass slide culture medium, put it in a petri dish with wet gauze at the bottom, repeat 3 times for each gradient The second time, put them in a 25°C incubator for cultivation, and observe the germination...

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Abstract

The invention relates to a culture medium for improving germination and growth of Luntai white apricot blossom pollen. The culture medium is prepared by respectively adding gibberellins, heteroauxin, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid or 6-benzyl adenine by taking agar, saccharose and boracic acid as basic culture medium components, and culture conditions comprise culture temperature and a pH value. According to the culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen, the technical problems that the germination rate of the pollen is low and the growth of a pollen tube is slow are solved, and an effective path for improving the germination rate is found for Luntai white apricot blossoms. According to the culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen, the germination and the growth of the Luntai white apricot blossom pollen are increased. The culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen has simplicity and convenience in operation, low cost, a short period and high germination rate and has a wide application prospect in stone fruit tree biology.

Description

technical field [0001] The invention relates to the technical field of fruit tree pollination and fertilization, in particular to a medium for improving the germination and growth of Luntai white apricot pollen. Background technique [0002] Apricot (Armeniaca vulgaris Lam) is a small deciduous tree, Rosaceae, Prunus. Apricot fruit is rich in vitamin A, vitamin C, carbohydrates, citric acid, malic acid and other nutrients. Thirst, heat-clearing and detoxifying, has certain health care and anti-cancer effects, and is favored by people no matter it is eaten fresh or processed. Chinese apricots are widely distributed in Hebei, Beijing, Henan, Shandong, Shanxi, Shaanxi, Gansu, Xinjiang and other places. There are more than 2,000 varieties, including 117 varieties in Xinjiang, and the most famous one is the small white apricot. Luntai White Apricot (New S-SV-PA-017-2009) is one of the most distinctive fine varieties in Xinjiang. It is also called Aximixi by local residents in Xi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 廖康孙慧瑛安晓芹耿文娟
Owner XINJIANG AGRI UNIV
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