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Method for remediating polycyclic aromatic hydrocarbon (PAH) polluted soil through cooperation of alfalfa and complex microbial inoculants

The technology of alfalfa and compound inoculum is applied in the field of bioremediation of polluted soil, which can solve the problems of low soil remediation efficiency, no proposal, and shortening of plant growth cycle, so as to restore the diversity and stability of ecological functions, and change the physiological contour. , the effect of broad market application prospects

Inactive Publication Date: 2016-01-20
周世永
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Patent CN200510038453.4 proposes to inoculate rhizobia and arbuscular mycorrhizal fungi while planting leguminous plants on polluted soil to promote the degradation of polycyclic aromatic hydrocarbons such as polychlorinated biphenyls in the soil, but does not propose how to shorten the plant growth cycle method, which reduces the efficiency of soil remediation

Method used

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  • Method for remediating polycyclic aromatic hydrocarbon (PAH) polluted soil through cooperation of alfalfa and complex microbial inoculants

Examples

Experimental program
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Embodiment 1

[0020] 1. Pretreatment of alfalfa seeds: cleanly select alfalfa seeds that are plump, insect-free, and mildew-free, soak in 75% absolute ethanol for 0.5min, take them out and place them in 0.1mol / LHgCl 2 Soak in the solution for 20 minutes, then rinse with sterilized water for 5 times, dry the surface moisture with gauze, then transfer to a petri dish for germination at room temperature;

[0021] 2, the preparation of Trichoderma spore suspension: Trichoderma T30 (Trichodermakoningii) bacterial classification is inoculated in high-sugar medium, and described high-sugar medium raw material composition number is: 1.5 parts of ammonium tartrate, 20 parts of glucose, phosphoric acid 1.0 parts of potassium dihydrogen, 1.0 parts of magnesium sulfate, 0.01 parts of ferric chloride, 50 parts of potatoes, 10 parts of agar, 1.0 parts of bran, 1.0 parts of corn juice, 1.0 parts of peptone, 1000 ml of distilled water, pH 7.0. Cultivate at 28°C for 4 days to produce a large number of spore...

Embodiment 2

[0043] 1. Pretreatment of alfalfa seeds: cleanly select alfalfa seeds that are plump, insect-free, and mildew-free, soak in 75% absolute ethanol for 1.0min, take them out and place them in 0.1mol / LHgC1 2 Soak in the solution for 25 minutes, then rinse with sterilized water for 5 times, dry the surface moisture with gauze, then transfer to a petri dish for germination at room temperature;

[0044] 2, the preparation of Trichoderma spore suspension: Trichoderma T30 (Trichodermakoningii) bacterial classification is inoculated in high-sugar medium, and described high-sugar medium raw material composition number is: 1.6 parts of ammonium tartrate, 18 parts of glucose, phosphoric acid 0.8 parts of potassium dihydrogen, 0.5 parts of magnesium sulfate, 0.05 parts of ferric chloride, 40 parts of potatoes, 15 parts of agar, 0.8 parts of bran, 0.5 parts of corn juice, 0.8 parts of peptone, 1000 ml of distilled water, pH 7.0. Cultivate at 28°C for 5 days to produce a large number of spore...

Embodiment 3

[0051] 1. Pretreatment of alfalfa seeds: cleanly select alfalfa seeds that are plump, insect-free, and mildew-free, soak in 75% absolute ethanol for 0.8min, take them out and place them in 0.1mol / LHgCl 2 Soak in the solution for 30 minutes, then rinse with sterilized water for 6 times, dry the surface moisture with gauze, then transfer to a petri dish for germination at room temperature;

[0052] 2, the preparation of Trichoderma spore suspension: Trichoderma T30 (Trichodermakoningii) bacterial classification is inoculated in high-sugar medium, and described high-sugar medium raw material composition number is: 1.6 parts of ammonium tartrate, 23 parts of glucose, phosphoric acid 1.5 parts of potassium dihydrogen, 1.5 parts of magnesium sulfate, 0.03 parts of ferric chloride, 60 parts of potatoes, 40 parts of agar, 6.0 parts of bran, 0.6 parts of corn juice, 2.8 parts of peptone, 1000 ml of distilled water, pH 7.0. Cultivate at 28°C for 5 days to produce a large number of spore...

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Abstract

The invention discloses a method for remediating polycyclic aromatic hydrocarbon (PAH) polluted soil through cooperation of alfalfa and complex microbial inoculants. Alfalfa seeds are pretreated, and the plant sprouting period is shortened by 2-3 days, so that the tolerance of the plant to soil containing PAHs is enhanced, and the remediating capacity of the plant on the soil is improved. Besides, in an alfalfa-trichoderma-nodule bacterium combined remediating system, the synergistic effect of trichoderma and nodule bacteria is used for promoting degradation of the alfalfa to the PAHs in the polluted soil. Meanwhile, the physiological contour of the microbial population level of alfalfa rhizosphere soil is changed, and the diversity and the stability of the microbial ecological functions of the soil are recovered, so that the growth environment of crops is improved, and the quality and the yield of the crops are improved; the method has wide market application prospects.

Description

technical field [0001] The invention relates to a bioremediation technology of polluted soil, in particular to a method for remediating soil polluted by polycyclic aromatic hydrocarbons with alfalfa synergistic compound bacterial agent. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic aromatic hydrocarbons, PAHs) are a class of hydrocarbons composed of two or more benzene rings fused together in a linear, angular or clustered form. It has cytotoxicity, genotoxicity, immunotoxicity, teratogenicity and carcinogenicity. In recent years, it has attracted more and more attention from people. With the acceleration of modern urbanization and the large-scale development and use of petroleum, coal and other energy sources, the pollution of polycyclic aromatic hydrocarbons (PAHs) is becoming more and more serious. In addition, the three waste purification treatment systems in many factories are not perfect, resulting in the flow of industrial wastewater into...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 周世永
Owner 周世永
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