Biological restoration method for p-chloronitrobenzene compound polluted environment

A technology of chloronitrobenzene and p-chloronitrobenzene, which is applied in the restoration of polluted soil, chemical instruments and methods, biological water/sewage treatment, etc. Effect

Active Publication Date: 2006-07-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technical solution involves combining different techniques like both chemical methods (chemostimulation), biofilm formation, enzymes, etc., together to efficiently remove pescates such as quaternary nitrophenol compound(QNPC). These combinations help increase efficiency at decompositing harmful substances found naturally within environmental matrices containing these agents without producing other unwanted side products. Additionally, they may stimulate the production of certain hydroxylic acid compounds called strawberry extracts, providing benefits related to improving crop health and reducing resistance against diseases caused by them. Overall, this innovative technique helps protect natural ecosystems and create valuable resources for future applications.

Problems solved by technology

This patented technical solution described involves finding ways to reduce harmful effects caused from industrial processes involving fertilizers containing certain types of organics called phytosulfonic acid(PSO). Phosphorus plays another crucial part in this process because they help break down sulfurized metal oxidations like iron hydrogen peroxy radicals, reducing oxygen consumption while rebuilding cellular structures. PBO represents one type of natural product found naturally throughout nature where many species live together. Overall, the challenge presented in current methods lies within identifying effective treatments against unwanted biological pollutions associated with heavy industries.

Method used

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  • Biological restoration method for p-chloronitrobenzene compound polluted environment
  • Biological restoration method for p-chloronitrobenzene compound polluted environment

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, detect the repair ability of the inventive method to the soilless substrate polluted by p-chloronitrobenzene compounds

[0030] Taking p-chloronitrobenzene as an example, the method for detecting the repair ability of the soilless substrate polluted by p-chloronitrobenzene compounds, the specific process comprises the following steps:

[0031] 1) Inoculate Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in an inorganic salt medium with p-chloronitrobenzene as a substrate, and vibrate at 28°C and 130rpm for 16 hours to obtain seeds liquid;

[0032] 2) Inoculate the seed solution of Comamonas testosteroni CNB-1 CGMCC No.1028 obtained in step 1) into LB liquid medium at an inoculation ratio of 1%, and culture it with shaking at 30° C. for 18 hours;

[0033] 3) Collect the bacteria by centrifugation at 6000rpm, and resuspend it in a sterile solution with a mass percent concentration of 0.9% NaCl to make the OD 600 Reach 2.0, obtain the ba...

Embodiment 2

[0037] Embodiment 2, detection method of the present invention is to the remediation ability of the soil polluted by p-chloronitrobenzene compounds

[0038] Taking p-chloronitrobenzene as an example, the pot experiment is used to detect the repair ability of the inventive method to the soil polluted by p-chloronitrobenzene compounds, and the specific process comprises the following steps:

[0039] 1) Inoculate Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in an inorganic salt medium with p-chloronitrobenzene as a substrate, and vibrate at 30°C and 130rpm for 16 hours to obtain seeds liquid;

[0040] 2) Inoculate the seed solution of Comamonas testosteroni CNB-1 CGMCC No.1028 obtained in step 1) into LB liquid medium at an inoculation ratio of 1%, and culture it with shaking at 28° C. for 18 hours;

[0041] 3) Collect the bacteria by centrifugation at 7000rpm, and resuspend it in a sterile solution with a mass percent concentration of 0.9% NaCl to make th...

Embodiment 3

[0046] Embodiment 3, Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in the detection of alfalfa root colonization ability

[0047] In order to facilitate the observation of the colonization of p-chloronitrobenzene-degrading strain Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in the plant root system during the bioremediation process, green fluorescent protein (green fluorescence protein, GFP ) gene is integrated on the genomic DNA of Comamonas testosteroni (Comamonas testosteroni) CNB-1, and specific method is: (1) preparation of Comamonas testosterone (Comamonas testosteroni) CNB-1 competent cell: summary of the invention The seed solution obtained in step 1) was inserted into LB liquid medium according to the inoculation amount of 1%, and cultured with shaking at 130rpm at 28°C to make its OD 600reach 0.3-0.4, (the following operations are all carried out at 4°C) collect the bacterial cells by centrifugation at 4500rpm, resuspend t...

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Abstract

The invention discloses a biological restoring method of p-chloronitrobenzene polluting environment, which comprises the following steps: immersing the alfalfa seed with root in the OD600 1.8-2.2 (Comamonas testosteroni) CNB-1 CGMCC No.1028 bacteria suspension liquid to precede uterus; cultivating the alfalfa seed in the p-chloronitrobenzene polluting environment to restore the environment. The invention displays significant action of p-chloronitrobenzene polluted environment, which possesses key real appliance meaning.

Description

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Claims

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Application Information

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Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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