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Method for stopping clover seeds dormancy in hard seeds

A seed and alfalfa technology is applied in the field of agriculture and animal husbandry, which can solve the problems of high processing time, high temperature, high concentration, high processing pressure, chemical method to seeds, unsatisfactory processing effect, etc., and achieves low cost, easy acquisition and cultivation, The effect of increasing the germination rate

Inactive Publication Date: 2008-10-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the above method to break the hard dormancy of Papilionaceae forage seeds, for seeds of different species, varieties, different maturity and different years of storage, the treatment time (sun exposure), treatment temperature and concentration (hot water soaking, chemical treatment) And processing pressure (physical milling) etc. are all very different, are not easy to control, and chemical methods are also harmful to seeds; in production practice, the processing effect is often not satisfactory; , there is currently no ideal solution for

Method used

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  • Method for stopping clover seeds dormancy in hard seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment one, adopt penicillium to process

[0032] Step 1, prepare carboxymethyl cellulose medium in proportion: carboxymethyl cellulose (g): yeast extract (g): MgSO 4 ·7H 2 O(g):KH 2 PO 4 (g): agar (g): distilled water (ml) = 30:2:1:2:30:2000.

[0033] Dissolve each component in distilled water in turn, shake well while adding, sterilize at 121°C for 15 minutes, and wait for the culture medium to condense before use.

[0034] Step 2, inoculate the Penicillium (Penicillium) bacterial classification on the above-mentioned carboxymethyl cellulose medium (provided by the bacterial strain preservation room of the Microbiology Laboratory, School of Life Science, Northwest A&F University); the control group is connected with sterile water; at 25 ° C Cultivate at ~30°C for 2 to 5 days, depending on the degree of growth.

[0035] Step 3, the fresh alfalfa seeds (produced in Jiuquan, Gansu Province, the variety is Gannong No. 3) harvested in the same year were disinfecte...

Embodiment 2

[0039] Embodiment two, adopt Aspergillus oryzae to process

[0040] Step 1, prepare carboxymethyl cellulose medium in proportion: carboxymethyl cellulose (g): yeast extract (g): MgSO 4 ·7H 2 O(g):KH 2 PO 4 (g): agar (g): distilled water (ml) = 30:2:1:2:30:2000.

[0041] Dissolve each component in distilled water in turn, shake well while adding, sterilize at 121°C for 15 minutes, and wait for the culture medium to condense before use.

[0042] Step 2, inoculate Aspergillus oryzae (Aspergillus oryzae) bacterial classification (provided by the strain preservation room of Microbiology Laboratory, School of Life Science, Northwest A&F University); the control group is connected with sterile water; cultivated at 25° C. to 30° C. for 3 to 7 days, To the degree of full growth.

[0043] Step 3, the fresh alfalfa seeds (produced in Jiuquan, Gansu Province, the variety is Gannong No. 3) harvested in the same year were disinfected by soaking the seeds in 70-75% alcohol for 5-10 minu...

Embodiment 3

[0047] Embodiment three, adopt penicillium+trichoderma viride to process

[0048] Step 1, prepare carboxylated cellulose medium according to the ratio: carboxymethylcellulose (g): yeast extract (g): MgSO 4 ·7H 2 O(g):KH 2 PO 4 (g): agar (g): distilled water (ml) = 30:2:1:2:30:2000.

[0049] Dissolve each component in distilled water in turn, shake well while adding, sterilize at 121°C for 15 minutes, and wait for the culture medium to condense before use.

[0050] Step 2, inoculate Penicillium+Trichoderma viride (provided by the strain preservation room of Microbiology Laboratory, School of Life Science, Northwest Agriculture and Forestry University), in a ratio of 1:1; the control group is connected with sterile water; at 25 ° C ~ 30 ° C Cultivate for 2 to 7 days, depending on the degree of growth.

[0051] Step 3, the fresh alfalfa seeds (produced in Jiuquan, Gansu Province, the variety is Gannong No. 3) harvested in the same year were disinfected by soaking the seeds i...

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Abstract

The invention discloses a method for breaking dormancy in hard seeds of clover; inoculation of Penicillum, Aspergillus oryzae, Trichoderma viride, or the combination of the strains is adopted in the method for treating the seeds of clover. The method solves the main problem for breaking dormancy in hard seeds of clover and can improve the germination rate and emergence rate of the seeds; the used fungi of microorganism can be made into coating agent of clover seeds or seed dressing microbial inoculum and used in the production directly, thus reducing some links for breaking hard seeds before sowing, and saving materials and manpower. Meanwhile, the method has low production cost, high benefit and convenient use. The method has good market prospect in restoring the vegetation of the degenerated grassland, ecology construction and the production of grassland agriculture.

Description

technical field [0001] The invention belongs to the field of agriculture and animal husbandry, and relates to a method for producing alfalfa seeds, in particular to a method for breaking the hard dormancy of alfalfa seeds. Background technique [0002] Alfalfa (Medicago L.), Fabaceae, Papilionaceae, Medicago genus, widely distributed in Europe, Asia, America and Africa, there are more than 60 species in the world, 16 species in my country, widely distributed, wild and cultivated . Alfalfa is the earliest and most widely planted perennial herb in the world. It has been cultivated for more than 2,000 years after it was introduced into my country via the "Silk Road". regions, even on the Qinghai-Tibet Plateau, the roof of the world. Alfalfa is known as the "King of Herb Grass". It not only has a high yield, but also has a good grass quality, and it is liked by all kinds of livestock and poultry. With the development of commodity economy, the scale of alfalfa industrialization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00C12N1/14C12R1/69C12R1/80C12R1/885
Inventor 王佺珍韩建国崔健边巴卓玛苗彦军吴春会
Owner NORTHWEST A & F UNIV
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