Method for breaking hard seed dormancy in astragalus seeds
A technique for soaking Astragalus seeds and seeds, which is applied in the fields of seed and rhizome treatment, application, agriculture, etc. It can solve the problems of large differences in treatment time, temperature and concentration treatment pressure, unsatisfactory treatment effect, and difficult to control.
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Embodiment 1
[0017] 1. Get raw material preparation culture medium by following weight ratio: guar gum: peptone: MgSO4 7H2O: KH2PO4: agar: distilled water=30:2:1:2:30:2000; Add and shake well, sterilize at 121°C for 15 minutes, and wait for the medium to condense before use;
[0018] 2. mold is inoculated on the above-mentioned guar gum medium, and described mold is one of Trichoderma harzianum Trichoderma, Aspergillus oryzae, Aspergillus niger, or the mixture of two or two of the above-mentioned bacterial classifications, or the mixture of the three of the above-mentioned bacterial classifications ;Cultivate at 25°C for 2 days, and the degree of mold growth is sufficient;
[0019] 3. Process astragalus seeds:
[0020] ①Sterilize the seeds of Astragalus membranaceus by soaking them in 70% alcohol for 5 minutes, and wash them twice with sterile water;
[0021] ②When the medium is covered with microorganisms, sprinkle Astragalus seeds in it at a density of 26 grains / cm2, and keep it at 25°...
Embodiment 2
[0026] 1. Get raw material preparation culture medium by following weight ratio: guar gum: peptone: MgSO4 7H2O: KH2PO4: agar: distilled water=30:2:1:2:30:2000; Add and shake well, sterilize at 121°C for 15 minutes, and wait for the medium to condense before use;
[0027] 2. mold is inoculated on the above-mentioned guar gum medium, and described mold is one of Trichoderma harzianum Trichoderma, Aspergillus oryzae, Aspergillus niger, or the mixture of two or two of the above-mentioned bacterial classifications, or the mixture of the three of the above-mentioned bacterial classifications ;Cultivate at 30°C for 7 days, and the degree of mold growth is sufficient;
[0028] 3. Process astragalus seeds:
[0029] ①Sterilize the seeds of Astragalus membranaceus by soaking them in 75% alcohol for 10 minutes, and wash them 3 times with sterile water;
[0030] ②When the medium is covered with microorganisms, sprinkle Astragalus seeds in it at a density of 34 grains / cm2, and keep it at ...
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