The invention discloses a novel method for preparing S-pantoprazole and salt, comprising the following steps: chiral separation reagents S-1,1,2-triphenyl-1,2-glycol and racemic pantoprazole react in an appropriate solvent, and the S-pantoprazole.S-1,1,2-triphenyl-1,2-glycol compound deposition is produced by filtering; the compound is dissociated by using alkali to remove S-1,1,2- triphenyl-1,2- glycol, then is neutralized by using acid, and neutral S-pantoprazole is obtained by filtering; and if S-pantoprazole salt is further required to be prepared, the S-pantoprazole prepared in the step (3) reacts with corresponding alkali in an appropriate solvent to obtain the S-pantoprazole salt. The method has high yield, high proportion of single isomer, simple technology, and convenient industrial production.