Alfalfa seed rhizobia seed coating agent and applications thereof

A technology of rhizobium agent and rhizobia, which is applied in the direction of seed coating/seed dressing, application, organic fertilizer, etc., which can solve the problems of poor bacterial fertilizer effect and short survival time, and achieve dry weight per plant increase and low cost , The effect of simple production process

Inactive Publication Date: 2015-03-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, rhizobia still have the problems of short survival time and poor bacterial fertilizer effect in seed coating.

Method used

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  • Alfalfa seed rhizobia seed coating agent and applications thereof
  • Alfalfa seed rhizobia seed coating agent and applications thereof
  • Alfalfa seed rhizobia seed coating agent and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, utilize No. 1 alfalfa seed rhizobia seed coating agent in the middle alfalfa to prepare pill coat alfalfa seed

[0041] This embodiment discloses two kinds of specific Rhizobium seed coating agents of No. 1 alfalfa seeds in Zhonglu No. 1, which are seed coating agents A and B of No. The control of alfalfa seed rhizobia seed coating agent A: Zhonglu No.1 alfalfa seed rhizobia seed coating agent A control 1 and Zhonglu No.1 alfalfa seed rhizobia seed coating agent A control 2; two kinds of Zhonglu No.1 alfalfa The control of alfalfa seed rhizobia seed coating agent B: Zhonglu No. 1 alfalfa seed rhizobia seed coating agent B control 3 and Zhonglu No. 1 alfalfa seed rhizobia seed coating agent B control 4; Medicago No. 1 alfalfa seeds coated with agent were used as control. The composition, preparation method and influence on alfalfa of these No. 1 alfalfa seed rhizobia seed coating agents will be clarified below.

[0042] 1. Prepare pill-coated alfalfa seed...

Embodiment 2

[0099] Embodiment 2, the optimization of each component of No. 1 alfalfa seed rhizobium seed coating agent in Zhonglu

[0100] The present embodiment comprises the following 3 steps: 1. Screening for the best Sinorhizobium meliloti strains with the best symbiosis compatibility with Zhonglu No. 1 alfalfa; Carry out the molybdenum tolerance test; 3. Determine the concentration of ammonium molybdate according to the molybdenum resistance test of the tested strains in the second step, design different alfalfa seed rhizobium seed coating agents, and optimize the alfalfa seed rhizobium coating agent. The specific experimental methods and experimental results are as follows:

[0101] 1. Screening of the Sinorhizobium alfalfa strain with the best symbiotic compatibility with Zhonglu No. 1 alfalfa

[0102] Sinorhizobium meliloti ACCC17537, Sinorhizobium meliloti ACCC17558, Sinorhizobium meliloti ACCC17617, Sinorhizobium meliloti ACCC17631 and Sinorhizobium meliloti were obtained by hy...

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PUM

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Abstract

The present invention discloses an alfalfa seed rhizobia seed coating agent and a preparation method thereof. The alfalfa seed rhizobia seed coating agent of the present invention is prepared from raw materials containing a rhizobia agent, an adhesive and a substrate, wherein the rhizobia agent is formed by uniformly mixing a rhizobia liquid and a rhizobia adsorbent, the active ingredient of the rhizobia liquid is the Sinorhizobium meliloti, the mass content of ammonium molybdate in the rhizobia agent is 0.1-0.2%, and the ammonium molybdate content in 100 g of the rhizobia agent is (0.1-0.2) g and the Sinorhizobium meliloti content in 100 g of the rhizobia agent is more than or equal to 5*10<11> cfu according to the ratio of the ammonium molybdate in the rhizobia agent to the Sinorhizobium meliloti. According to the invention, the alfalfa rhizobia and the molybdenum with the optimal concentration are matched, and the skimmed milk is added to the adhesive as the protection agent, such that the prepared alfalfa seed pill coating agent has characteristics of rhizobia activity prolonging, plant nodulation and nitrogen-fixation promoting, substantial alfalfa yield increasing and alfalfa quality improving.

Description

technical field [0001] The invention relates to an alfalfa seed rhizobium seed coating agent and application thereof in the biological field. Background technique [0002] Alfalfa (Medicago sativa L.) is warm-loving, cold-resistant, widely adaptable, rich in nutrition, high in protein, and good in palatability. It is the most widely distributed cultivated grass in the world today. Alfalfa seeds are small and carry few nutrients. Seeds in a normal germination state have relatively weak resistance to adverse sowing conditions such as drought and cold. Therefore, before planting, the alfalfa seeds are generally treated with pill coating. [0003] Rhizobia are a class of Gram-negative aerobic bacteria that can live in symbiosis with legumes, form root nodules and fix nitrogen in the air to provide nutrients for plants. But at present, rhizobia still have the problems of short survival time and poor bacterial fertilizer effect in seed coating. Contents of the invention [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01C1/06
CPCA01C1/06C05B1/02C05G3/00C05C3/00C05D3/02C05D9/02C05F11/02
Inventor 邓波周冀琼
Owner CHINA AGRI UNIV
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