46 results about "Epicotyl" patented technology

The invention discloses a seed multiplication and rapid cultivation method of a Paris polyphylla Smith that belongs to the paris of liliaceae, which is that: the seeds obtained during October and November are sent into the environment under temperature 5+/-1 DEG C, and taken out after one month; red arils of the seeds are rubbed off, then the seeds are washed and repeatedly sent into the environment under temperature 20-22 DEG C and 5+/-1 DEG C for twice; after the process, the seeds obtained are sowed. The invention forms imitation ecological environment by adopting artificial environment to imitate natural environment, so as to promote seed and embryo after-ripening, rescind epicotyl dormancy of Paris Polyphylla Smith and achieve the purpose of seed normal bourgeon; by adopting the method, the whole germination period only needs about 270 days, thus shortening the germination period of the Paris polyphylla Smith.

The invention discloses a polygonatum cyrtonema seed rapid emerging method. Polygonatum cyrtonema fruits are harvested and washed after the fruits are mature to obtain clean seeds for cold sand storage immediately, dormancy is removed from hypocotyl after storage treatment of 120 days, the seeds are treated with gibberellin with proper concentration and soaking time and are not sprouted, and emerging doesn't occur in the current year; gibberellin treated seeds are sown in a seedling plate loaded with a loose, fertile and moist substrate, primary roots of polygonatum cyrtonema are grown fully after four months, dormancy is removed from epicotyl after the primary roots are taken out and treated with cytokinin 6-BA with proper concentration and soaking time, the seeds are transplanted in a shading seedling field, emerging starts after about 15 days, the emerging is uniform, and the emergence rate can be as high as 70%. The polygonatum cyrtonema seed rapid emerging method has the advantages that the seed germination rate is high after treatment, the emergence rate is high, and the seedling culture time is reduced by one year.

The invention relates to a method for promoting paeonia suffruticosa seedling growth and application thereof. Specifically, the invention provides a paeonia suffruticosa seedling treatment fluid, which comprises: a paeonia suffruticosa seedling growth active agent (gibberellin and/or 6-benzyladenine) and a surfactant, and also can include an agronomically acceptable soluble calcium salt. The growth active agent can break epicotyl dormancy of paeonia suffruticosa seeds, improve the germination rate/germination potential of paeonia suffruticosa seeds, accelerate the paeonia suffruticosa seed growth process, and promote seedling emergency or flowering of paeonia suffruticosa seed seedlings/tissue culture seedlings. The surfactant can make active substances fully enter cells. And the soluble calcium salt is closely related to multiple physiological functions of cells.

The invention discloses a cultivation method for rapidly cultivating paeonia ostii seedlings. The method is characterized by comprising the following steps: (1) selecting, cleaning and disinfecting seeds; (2) depositing the seeds into wet sand; (3) manually breaking the epicotyl dormancy of paeonia ostii seeds in advance; (4) cultivating the seeds into seedlings; (5) planting in fields; and (6) performing field management. According to the cultivation method, the epicotyls dormancy of the paeonia ostii seeds are broken in advance by applying a manual method, the paeonia ostii seeds after the epicotyls dormancy breaking are seeded in a greenhouse and can emerge at 11-12 months after seeding, and the field planting is performed before or after the mid of March in the next spring. By adopting the method, the seedling emergence time of paeonia ostii seedlings is brought forward by 3-4 months, the planting is brought forward by 7-8 months in comparison to conventional field seeding, and soil occupation and management labor force in seedling cultivation are reduced effectively. Meanwhile, a substrate is used in rapid seedling, so that diseases and pest infection of the seedlings can be reduced greatly, plant diseases and insect pests are reduced, the cost is reduced, and the rapid development of the paeonia ostii industry is promoted effectively.

The invention discloses a method for improving citrus canker resistance based on CRISPRCas9 mediated CsWRKY22 site-directed editing, and belongs to the field of plant genetic engineering. A CRISPR/Cas9 editing vector guided by S1 and S2 sg RNA is constructed, the epicotyl of wanjicheng orange is transformed with an agrobacterium-mediated method, so that the function of CsWRKY22 protein is lost or reduced, successful editing of CsWRKY22 gene in the citrus genome is realized, CsWRKY22 mutant transgenic plants are obtained, plant resistance to citrus canker is enhanced, and citrus with high canker resistance is obtained.

The invention relates to a fast propagation method for aquatic plant lotus flowers. According to the method, in vitro tissue culture is firstly adopted for obtaining tissue culture seedlings, and the in vitro tissue culture comprises the following steps that: (1) lotus flower seeds are used as initial raw materials, after the lotus flower seeds are subjected to sterilization and disinfection treatment, plumules are taken out under the aseptic condition and are inoculated into a basic culture medium to be cultured for 2 to 3 weeks, and aseptic seedlings are obtained; (2) the hypocotyl upper parts and cotyledons of the aseptic seedlings containing growth point parts are cut off and are inoculated into a propagation culture medium, the light illumination culture is carried out for 3 to 4 weeks, and tissue culture calluses are obtained; (3) buds with the length being 2 to 3cm in the tissue culture calluses obtained in the second step are cut off and are inoculated into a strong seedling culture medium to be cultured; and (4) after the seedlings in the third step grow to 4 to 6cm, the seedlings are transplanted onto a rooting culture medium for carrying out rooting culture, and the tissue culture seedlings are obtained. The fast propagation method provided by the invention solves the problems of few lotus root resources and long propagation period in the prior art, and a new path is provided for meeting the market requirements.

The invention discloses a method for cultivating a grafted tomato seedling with two sprouts. The method mainly includes the steps that a stock species which is resistant to stress and high in water absorption capacity and fertilizer absorption capacity and a scion species which is high in yield and good in quality are selected, the stock and the scion are cultivated to have 2-4 true leaves separately, tube grafting is conducted, after the grafted seedling completely survives, a cutter is used for making a horizontal slash on the embryonal axis portion of the scion of the grafted seedling, the true leaves and an apical growing point are removed, one two cotyledons are maintained, the grafted seedling continues to be cultivated to sprout in the axil, and two side branches are generated. Compared with a traditional grafted tomato seedling, the two side branches which are uniform in growth vigor can be generated, after planting, the two side branches become two fruit bearing main vines, and the good-quality and high-yield cultivation of tomatoes is easily achieved.

The invention discloses a paeonia rockii seed accelerating germination method which comprises a paeonia rockii epicotyl dormancy breaking method and a paeonia rockii hypocotyl dormancy breaking method; the paeonia rockii hypocotyl dormancy breaking method comprises the followings: soaking sterilized paeonia rockii seeds with GA3 with the concentration of 100-700 mg/L and carrying out stratification treatment on a substrate; the paeonia rockii epicotyl dormancy breaking method comprises the followings: selecting seeds with the root length greater than or equal to 0.5 cm and less than or equal to 3 cm, carrying out accelerating germination treatment on the seeds respectively, soaking root-promoted paeonia rockii seeds with GA3 with the concentration of 100-400 mg/L, carrying out stratification treatment on the substrate, and placing the seeds at the constant temperature of 25 DEG C for accelerating germination and moisturizing. According to the invention, the double paeonia rockii seed dormancy breaking technologies are adopted, so that the germinating time is shortened, the seeds germinate after being sown for 97 days only, the highest emergence rate can reach 90% or above and the paeonia rockii seed accelerating germination method is suitable for large-scale promotion and cultivation.

The invention relates to a red pine tissue propagation method. The method comprises the following steps: taking a red pine asepsis seedling cotyledonary node (containing a cotyledon, a budding epicotyl and a 6 to 8mm hypocotyl) as an explant which is inoculated in a GD+6-BA3.0 to 5.0mg/L+NAA0.1 to 0.3mg/L culture medium so as to induce a cotyledon base to generate clustered seedlings; after 4 to 5 weeks, shifting the explant together with the differentiated clustered seedlings into a culture medium with added active carbon or NAA so as to promote the elongation of the seedlings; and when the clustered seedlings grow to 1.5 to 2.0cm, cutting off the seedlings separately, cutting the seedlings into 5 to 8mm small sections, and inoculating the small sections in a GD+6-BA1.5 to 2.5mg/L+NAA0.25mg/L culture medium for further inducing the propagation of clustered seedlings. The propagated clustered seedlings can be used for a new cycle of propagation again after culture and elongation. The method can realize the clustered seedlings propagation coefficient of between 3.5 and 4.6; and a seed can propagate 20 to 30 thousand tissue culture seedlings for rootage 2 years later.

The invention discloses a method for releasing dormancy of oil-used peony seeds. The method comprises the following steps: a quick post-maturation stage of seed embryos of the oil-used peony seeds: harvesting peony pods, spreading the peony pods in shade indoors, and overturning up and down once very day; after the pods crack naturally, screening and collecting shed seeds; performing stratification treatment after full seeds which submerge in water are dried in shade indoors for 1 to 2 days; adding fresh river sand which contains 50 to 60 mass percent of water in the screened seeds, and spreading a mixture indoors for drying; keeping internal temperature and humidity of a mixture of the seeds and the river sand, and treating for 30 days to obtain the mixture of the seeds with the seed embryos which are completely matured and the river sand; a hypocotyledonary axis dormancy releasing stage of the oil-used peony seeds: controlling the indoor temperature of the mixture of the seeds and the river sand to be between 15 and 20 DEG C, keeping the humidity of the river sand, and treating for 30 days to obtain a mixture of the seeds of which the root length is greater than 4 centimetres and the river sand; an epicotyl dormancy releasing stage of the oil-used peony seeds: controlling the indoor temperature of the mixture of the seeds of which the root length is greater than 4 centimetres and the river sand to be below 10 DEG C; spraying a plant growth conditioning agent; keeping the river sand at a certain humidity; treating for 30 days; completing a dormancy releasing process of the oil-used peony seeds.

The invention relates to a method for establishing an agrobacterium rhizogene mediated peanut root inducing system, and application thereof, belonging to the field of biotechnology. According to the invention, the peanut root system is induced by using agrobacterium rhizogene and a microinjection method to obtain a chimeric plant; simultaneously, the method of the system is optimized, i.e. the peanut is infected by performing microinjection on the epicotyl as an explant; the highest conversion efficiency is 61%; and simultaneously, the bioactivities of cry8Ea1 and cry8Ha1 genes optimized by codon on the larvae of holotrichia parallela are verified. The invention successfully establishes a peanut root inducing system, provides a quick verification method for inducing a peanut plant from an extraneous gene, and provides a technical support for further acquiring genetically modified peanut plants.

The invention discloses a paper tube seedling cultivation method for radix paeoniae rubra in a northern region. A greenhouse paper tube seedling cultivation technology is adopted in the paper tube seedling cultivation method. The paper tube seedling cultivation method comprises the steps: sowing seeds and finishing radicle development and rooting in autumn; breaking the dormancy of epicotyl in a greenhouse in a low-temperature state in winter; cultivating strong seedlings in the greenhouse in spring; starting to transplant the seedlings from the last ten days of May to the first ten days of June in the next year; and carrying out harvesting within 3-4 years after field planting. By using the paper tube seedling cultivation method, early field planting can be realized, and the radix paeoniae rubra can be harvested one year ahead of schedule; and paper tube greenhouse seedling cultivation is adopted, so that root systems are not damaged during transplanting, the seedling cultivation survival rate and the transplanting survival rate can be favorably increased, the root systems of radix paeoniae rubra seedlings are developed, and the seedlings are healthy and strong.

A technique for directly polygemmic regenerative plant of cotton by in vitro culture of its epicotyl explant and a decapitated epicotyl explant are disclosed.

The invention provides a method for quickly breaking the epicotyl dormancy of Rhizoma Paridis. The method comprises the following steps: harvesting seeds at a most suitable time to ensure that Paris polyphylla seeds for seedling growth are seeds with an excellent quality; timely removing easily decayed seed testae to prevent the seeds from being invaded by moulds; and air-drying the Paris polyphylla seeds, swelling the air-dried Paris polyphylla seeds to soften the testae, facilitate the activity of seed endoenzymes, promote the hydrolysis of stored substances, dissolve and extract germination-inhibiting substances in the seeds and facilitate the breaking of the dormancy of seeds. Soaking of the seeds in boiling water can achieve high temperature disinfection, few diseases and few pesticides, has certain damages to hard testae, increase the permeability of the seeds, further promotes the conversion of the stored substances in the seeds into nutritional substances which can participatein metabolism, and effectively promotes the germination of the seeds.

The invention belongs to the technical field of plant genetic engineering transformation and discloses a genetic transformation method for stab-vacuum infiltration-assisted agrobacterium tumefaciens-mediated castor seeds. The method comprises the steps of pre-culturing castor seeds, stabbing epicotyls of the pre-cultured castor seeds, performing vacuum infiltration for enabling an agrobacterium tumefaciens solution containing target genes to infect the castor seeds, performing co-culture, removing agrobacterium tumefaciens, and performing transplantation, wherein according to the vacuum infiltration, the stabbed seeds are treated in the agrobacterium tumefaciens solution for 5-20 minutes under the vacuum negative pressure of -30kPa to -70kPa, air is discharged for 2-5 minutes, and the stabbed seeds are treated for 2-5 minutes under the negative pressure of -30kPa to -70kPa again. According to the method, the pre-cultured seeds are directly taken as receptors, so that in vitro tissue culture is not required; and a transgenic plant can be obtained in 15 days from castor seed pre-culture to extracted DNA detection, so that the transformation cycle is short, the transformation rate is high, the operation is relatively simple, and the repeatability is high.

The invention discloses a method for promoting efficient germination of seeds of a valuable and rare endangered plant yunnanopilia longistaminea, wherein the method comprises the steps: collecting ripe yunnanopilia longistaminea seeds; removing seed coats, and breaking inhibition of the seed coats; carrying out cleaning and disinfection treatment on the seeds; carrying out root promoting culture, and soaking the seeds with a 50 mg/L GA3 solution for 24 h; soaking the seeds with a 100 mg/L GA3 solution for 24 h, placing the seeds at the temperature of 5 DEG C, and refrigerating for 7-14 days to change endogenous hormone change of the yunnanopilia longistaminea in seed germination and seedling building processes, so as to break epicotyl dormancy; placing the seeds in the environment of the temperature of 28 DEG C, and culturing; culturing for 3-4 weeks to obtain seedlings having euphylla grown; and providing the seedlings for wild transplanting after growing for 8-10 weeks. The system method directly adopting the yunnanopilia longistaminea seeds for fast and efficient cultivation of the seedlings effectively relieves the epicotyl dormancy of the yunnanopilia longistaminea, ensures that the germination rate of the yunnanopilia longistaminea seeds is more than or equal to 70%, effectively shortens the seedling culture time by 60 days, and is simple and convenient to operate, high in survival rate, and strong in practicability. An effective technical means is provided for species protection biology, restoration ecology, resource exploitation and utilization and the like.

The invention discloses a method for releasing Tongling peony seed epicotyl dormancy. The method includes the steps of: 1) selecting seeds, 2) performing seed treatment; 3) conducting root promoting, and 4) when most of the root-promoted Tongling peony seed radicles grow to 2-4cm, selecting seeds with a root length of 3cm and putting the seeds in a 1-5DEG C environment to conduct low temperature treatment for 60 days; sowing the low temperature treated seeds in the last ten-day period of December; and carrying out routine management on the low temperature treated sowed seeds in a 16-20DEG C greenhouse for 20d. The method provided by the invention is simple and practical, has the characteristics of low cost and convenient management, can effectively release Tongling peony epicotyl dormancy after 60d of low temperature treatment on the basis of root promoting, the germination rate can reach 87.9%, and the shoot length is 6.2cm. Therefore, the method meets and suits large-scale popularization cultivation.