A kind of construction method of highland barley-endophytic fungus symbiont

Abstract

The present invention provides a method for constructing a highland barley-endophytic fungi symbiosis, comprising the following steps: (1) disinfection of highland barley seeds; (2) cultivation of sterile highland barley seedlings; (4) inoculation: on the sterile highland barley seedlings Artificial inoculation of the hypocotyl: vertically cut a small opening at the epicotyl of the sterile highland barley seedling, stuff the cultured mycelium into the small opening, and place it at a constant temperature (25±2)°C for dark cultivation for 7-10 days. Then at a constant temperature (25±2) °C, the light intensity is 2000~3000lx, and the light time is 16h·d ‑1 Cultivated under the conditions for 7-10 days to obtain highland barley seedlings; (5) transplantation: after the highland barley seedlings were washed root medium, they were transferred into the substrate and cultivated until the seeds were harvested; (6) endophytic fungi were detected: transplanted for a period of time The inoculation of endophytic fungi was then detected, the seedlings that were not successfully inoculated were eliminated, and the seeds were collected after the remaining seedlings were mature. The present invention constructs a highland barley-endophytic fungus symbiosis by inoculating grass endophytic fungi on the epicotyl of highland barley, and creates a new highland barley germplasm resource.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, and in particular relates to a method for constructing a highland barley-endophytic fungus symbiont. Background technique [0002] The dual characteristics of grass-endophyte symbionts have become one of the hot research areas in the past 20 years. It has been recognized by the international academic community that endophytic fungi can improve grass stress resistance and promote host growth. The infection of Neotyphodium endophytic fungi can also promote growth and improve competitiveness by enhancing drought resistance, salt tolerance, heavy metal tolerance and nutrient absorption; it can also increase host resistance to insects, nematodes, livestock, The resistance of wild animals, pathogenic fungi, and viruses affects the succession of grassland plant communities and changes the structure of the food chain. [0003] At present, about 10% of temperate grasses in the world have e...

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Problems solved by technology

[0004] At present, foreign countries have artificially inoculated pastures such as ryegrass and tall fescue, and successfully constructed artificial symbionts, but there is no report on the successful construction of highland barley-endophytic fungal symbionts

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