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Micrografting method for castor oil plants

A technology of micro-grafting and castor, which is applied in the field of plant biology, can solve the problems of castor's difficulty in regenerating adventitious buds and rooting

Pending Publication Date: 2020-05-19
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the difficult problem of castor-oil regeneration adventitious buds and resistant bud strip rooting, combine tissue culture and grafting, provide a kind of castor-oil micro-grafting method, establish a kind of effective castor-oil micro-grafting technology system to provide technical support for the cultivation of new varieties of castor

Method used

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  • Micrografting method for castor oil plants
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  • Micrografting method for castor oil plants

Examples

Experimental program
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Effect test

preparation example Construction

[0032] 3.1 Preparation of MS solid medium

[0033] Weigh 4g of macroelement mother liquor powder, measure 1mL of trace mother liquor, 10mL of organic mother liquor, and 10mL of iron salt mother liquor, weigh 0.1g of inositol and 30g of sucrose in a beaker, add distilled water to dissolve, stir evenly with a magnetic heating stirrer, Then set the volume to 1000mL, adjust the pH to 5.8-6.0, add 7g of agar powder, stir evenly, heat to boiling, then divide into culture bottles, and then sterilize at 121°C and 0.1MPa for 20min, then take it out to cool and solidify at room temperature spare.

[0034] 3.2 Obtaining rootstock and scion

[0035] After soaking the castor seeds for 4 hours, remove the shells, soak the shelled seeds with 75% ethanol aqueous solution for 1min, then soak with 2% sodium hypochlorite solution for 20min, then wash with sterile water for 3-5 times, and then sterilize The dehulled seeds were inoculated on the MS solid medium, the aseptic seedlings after culti...

Embodiment 1

[0047] Embodiment 1: IBA is to the influence of castor-oil plant grafting growth effect

[0048] The grafted seedlings were inoculated in MS medium supplemented with different concentrations of IBA, and the effect of IBA on the growth of castor seedlings was observed.

[0049] Table 1 The effect of IBA on the growth of castor-bean grafted seedlings

[0050]

[0051] Note: The letters after the data represent the significance analysis results.

[0052] From Table 1 and figure 2 (A-L) It can be seen that, from the perspective of promoting plant height and bud growth, the MS medium adding 0.1mg / L IBA is the most beneficial to the elongation growth of the scion and plant of castor-oil grafted seedlings, and the bud length increase amount reaches (1.77cm) and Plant height increase (2.03cm) and grafting survival rate (74.50%) all reach maximum value; From the promotion effect aspect to root number and root length, to add the MS medium of 0.6mg / L IBA to castor-bean grafted seed...

Embodiment 2

[0053] Embodiment 2: The influence of 6-BA on the growth effect of castor-oil plant grafted seedlings

[0054] The grafted seedlings were inoculated in MS medium supplemented with different concentrations of 6-BA, and the effects of 6-BA on the growth of castor seedlings were observed.

[0055] Table 2 Effect of 6-BA on the growth of castor seedlings grafted

[0056]

[0057] Note: The letters after the data represent the significance analysis results.

[0058] It can be known from Table 2 that adding BA in MS medium can inhibit the growth of castor plant grafted seedlings, and will inhibit the plant height and bud length, as well as the number of roots and root length, and the higher the BA concentration, the inhibition The effect is more obvious.

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Abstract

The invention discloses a micrografting method for castor oil plants. The method comprises the steps: selecting hypocotyls of sterile seed seedlings of the castor oil plants as rootstocks, selecting epicotyls with embryonic buds as scions, and carrying out micrografting, so as to obtain grafted seedlings; and then, culturing the grafted seedlings in a grafted seedling culture medium (an MS solid culture medium added with 0.1mg / L of IBA, 0.2mg / L of KT or 0.8mg / L of sodium nitrophenolate). By the micrografting method disclosed by the invention, the scions and the rootstocks heal to form completeplants which can grow and develop normally. According to the method, a period for genetic improvement on the castor oil plants is shortened, more robust plants are efficiently obtained in a short term, and a new way is provided for subsequent researching on the castor oil plants.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a castor-bean micro-grafting method. Background technique [0002] Castor (Ricinus communis L.), also known as hemp seed, kenaf, grass hemp, bull castor, etc., is a plant of the genus Castor in the Euphorbiaceae family. Castor is mainly distributed in tropical or subtropical regions, and its plants are shrubs or trees, which are annual herbaceous plants. There is a long history of planting castor in my country, and the output of castor ranks among the top in the world. Castor bean has a high oil content, about 46%-56%, and is the raw material for making high-quality industrial oil. Castor oil has the characteristics of high temperature resistance, high viscosity, and is not easy to solidify. It is widely used in aviation, aerospace, ships and other fields, as well as lubricating oil and protective oil for precision instruments. In addition, castor seeds contain t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01C1/00A01C1/08A01G31/00A01G24/20A01G24/30
CPCA01C1/00A01C1/08A01G31/00A01G2/30A01G24/20A01G24/30
Inventor 刘颖刘铠哲薛迎斌李小朋刘炳镇
Owner GUANGDONG OCEAN UNIVERSITY
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