Method for promoting paeonia suffruticosa seedling growth and application thereof

Abstract

The invention relates to a method for promoting paeonia suffruticosa seedling growth and application thereof. Specifically, the invention provides a paeonia suffruticosa seedling treatment fluid, which comprises: a paeonia suffruticosa seedling growth active agent (gibberellin and / or 6-benzyladenine) and a surfactant, and also can include an agronomically acceptable soluble calcium salt. The growth active agent can break epicotyl dormancy of paeonia suffruticosa seeds, improve the germination rate / germination potential of paeonia suffruticosa seeds, accelerate the paeonia suffruticosa seed growth process, and promote seedling emergency or flowering of paeonia suffruticosa seed seedlings / tissue culture seedlings. The surfactant can make active substances fully enter cells. And the soluble calcium salt is closely related to multiple physiological functions of cells.

Description

Technical field

[0001] The invention belongs to the field of plant biology. Specifically, the invention relates to a method for promoting the growth of peony seedlings and its application. Background technique

[0002] Peony (Paeonia suffruticosa), also known as rich flower, Bailiangjin, wood peony, squirrel, deer leek, Luoyang flower, is a woody plant of the genus Paeonia, a deciduous shrub in the family Paeoniaceae. The reputation of "The King" is the first choice for my country's national flower. Peony not only has high ornamental value, but also has good medicinal value. As early as the Han Dynasty, the medicinal plant "Shen Nong's Materia Medica" has records that peony was domesticated and used as medicine. With the increase of the market value of peony in landscaping and medicinal material reserve, the economic market of peony has been born. Enlarging the reproduction coefficient, improving the efficiency of propagation, carrying out large-scale production of peony seedli...

Examples

Embodiment 1

[0099] Speed ​​up the production of peony seedlings

[0100] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0101] 2. Take some clean river sand, sterilize it at 121℃ for 1h, cool it to room temperature, and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak in river sand, bury the peony seeds of step (1) in the above wet sand, store them at 4°C in the dark for 2 months, and then root and begin to germinate.

[0102] 3. Sow the rooted peony seeds in step (2) shallowly in a matrix of peat, vegetable garden soil and perlite (3:6:1), and grow them in a greenhouse at 28±2°C and natural light for 1 month , The petiole and leaves are beginning to appear. Bury the peony seeds of step (1) in the above wet sand, store them at 4° C. for 2...

Embodiment 2

[0107] Speed ​​up the production process of peony tissue culture seedlings

[0108] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0109] 2. Peel off the husks of peony seeds obtained in step (1), and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak for 24h, discard the liquid, collect seeds with endosperm, filter paper to absorb surface water.

[0110] 3. Wash the endosperm seeds obtained in step (2) with tap water, soak up the surface water droplets, disinfect with 70% ethanol for 30-90 seconds on an ultra-clean workbench, and soak in 0.1% mercury for 5-8 minutes , Rinse with sterile water 5-8 times, absorb water droplets on the sterile material surface with sterile filter paper, peel off the seed embryo, inoculate it in WPM ...

Technical field

[0001] The invention belongs to the field of plant biology. Specifically, the invention relates to a method for promoting the growth of peony seedlings and its application. Background technique

[0002] Peony (Paeonia suffruticosa), also known as rich flower, Bailiangjin, wood peony, squirrel, deer leek, Luoyang flower, is a woody plant of the genus Paeonia, a deciduous shrub in the family Paeoniaceae. The reputation of "The King" is the first choice for my country's national flower. Peony not only has high ornamental value, but also has good medicinal value. As early as the Han Dynasty, the medicinal plant "Shen Nong's Materia Medica" has records that peony was domesticated and used as medicine. With the increase of the market value of peony in landscaping and medicinal material reserve, the economic market of peony has been born. Enlarging the reproduction coefficient, improving the efficiency of propagation, carrying out large-scale production of peony seedli...

Examples

Embodiment 1

[0099] Speed ​​up the production of peony seedlings

[0100] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0101] 2. Take some clean river sand, sterilize it at 121℃ for 1h, cool it to room temperature, and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak in river sand, bury the peony seeds of step (1) in the above wet sand, store them at 4°C in the dark for 2 months, and then root and begin to germinate.

[0102] 3. Sow the rooted peony seeds in step (2) shallowly in a matrix of peat, vegetable garden soil and perlite (3:6:1), and grow them in a greenhouse at 28±2°C and natural light for 1 month , The petiole and leaves are beginning to appear. Bury the peony seeds of step (1) in the above wet sand, store them at 4° C. for 2...

Embodiment 2

[0107] Speed ​​up the production process of peony tissue culture seedlings

[0108] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0109] 2. Peel off the husks of peony seeds obtained in step (1), and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak for 24h, discard the liquid, collect seeds with endosperm, filter paper to absorb surface water.

[0110] 3. Wash the endosperm seeds obtained in step (2) with tap water, soak up the surface water droplets, disinfect with 70% ethanol for 30-90 seconds on an ultra-clean workbench, and soak in 0.1% mercury for 5-8 minutes , Rinse with sterile water 5-8 times, absorb water droplets on the sterile material surface with sterile filter paper, peel off the seed embryo, inoculate it in WPM ...

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